Extracellular Recording of Neuronal Activity Combined with Microiontophoretic Application of Neuroactive Substances in Awake Mice

Differences in the activity of neurotransmitters and neuromodulators, and consequently different neural responses, can be found between anesthetized and awake animals. Therefore, methods allowing the manipulation of synaptic systems in awake animals are required in order to determine the contributio...

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Autores: Aguilar Ayala, Yaneri, Pérez González, David, Duque Doncos, Daniel, Palmer, Alan R., Malmierca, Manuel S.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Universidad de Salamanca (USAL)
Repositorio:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/160458
Acceso en línea:http://hdl.handle.net/10366/160458
Access Level:acceso abierto
Palabra clave:Auditory
Stimulus-specific adaptation
Inferior colliculus
Frequency response area
Gabazine
Inhibition
Multibarrels
Tungsten electrode
Synaptic inputs
Inferior Colliculi
2490 Neurociencias
2411.13 Fisiología de la Audición
colículos inferiores
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spelling Extracellular Recording of Neuronal Activity Combined with Microiontophoretic Application of Neuroactive Substances in Awake MiceAguilar Ayala, YaneriPérez González, DavidDuque Doncos, DanielPalmer, Alan R.Malmierca, Manuel S.AuditoryStimulus-specific adaptationInferior colliculusFrequency response areaGabazineInhibitionMultibarrelsTungsten electrodeSynaptic inputsInferior Colliculi2490 Neurociencias2411.13 Fisiología de la Audicióncolículos inferioresDifferences in the activity of neurotransmitters and neuromodulators, and consequently different neural responses, can be found between anesthetized and awake animals. Therefore, methods allowing the manipulation of synaptic systems in awake animals are required in order to determine the contribution of synaptic inputs to neuronal processing unaffected by anesthetics. Here, we present methodology for the construction of electrodes to simultaneously record extracellular neural activity and release multiple neuroactive substances at the vicinity of the recording sites in awake mice. By combining these procedures, we performed microiontophoretic injections of gabazine to selectively block GABAA receptors in neurons of the inferior colliculus of head-restrained mice. Gabazine successfully modified neural response properties such as the frequency response area and stimulus-specific adaptation. Thus, we demonstrate that our methods are suitable for recording single-unit activity and for dissecting the role of specific neurotransmitter receptors in auditory processing. The main limitation of the described procedure is the relatively short recording time (~3 hr), which is determined by the level of habituation of the animal to the recording sessions. On the other hand, multiple recording sessions can be performed in the same animal. The advantage of this technique over other experimental procedures used to manipulate the level of neurotransmission or neuromodulation (such as systemic injections or the use of optogenetic models), is that the drug effect is confined to the local synaptic inputs to the target neuron. In addition, the custom-manufacture of electrodes allows adjustment of specific parameters according to the neural structure and type of neuron of interest (such as the tip resistance for improving the signal-to-noise ratio of the recordings).MINECO grants BFU201343608-P and PSI2013-49348-EXP JCYL grant SA343U14 MRC CONACyTMinisterio de Economía y Competitividad - MINECOJunta de Castilla y León - JCYLMRCCONACyTMyJove Corporation202420242016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10366/160458reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)InglésMinisterio de Economía y Competitividad - MINECO (BFU201343608-P)Ministerio de Economía y Competitividad - MINECO (PSI2013-49348-EXP)Junta de Castilla y León - JCYL (SA343U14)Attribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:gredos.usal.es:10366/1604582026-06-07T06:28:51Z
dc.title.none.fl_str_mv Extracellular Recording of Neuronal Activity Combined with Microiontophoretic Application of Neuroactive Substances in Awake Mice
title Extracellular Recording of Neuronal Activity Combined with Microiontophoretic Application of Neuroactive Substances in Awake Mice
spellingShingle Extracellular Recording of Neuronal Activity Combined with Microiontophoretic Application of Neuroactive Substances in Awake Mice
Aguilar Ayala, Yaneri
Auditory
Stimulus-specific adaptation
Inferior colliculus
Frequency response area
Gabazine
Inhibition
Multibarrels
Tungsten electrode
Synaptic inputs
Inferior Colliculi
2490 Neurociencias
2411.13 Fisiología de la Audición
colículos inferiores
title_short Extracellular Recording of Neuronal Activity Combined with Microiontophoretic Application of Neuroactive Substances in Awake Mice
title_full Extracellular Recording of Neuronal Activity Combined with Microiontophoretic Application of Neuroactive Substances in Awake Mice
title_fullStr Extracellular Recording of Neuronal Activity Combined with Microiontophoretic Application of Neuroactive Substances in Awake Mice
title_full_unstemmed Extracellular Recording of Neuronal Activity Combined with Microiontophoretic Application of Neuroactive Substances in Awake Mice
title_sort Extracellular Recording of Neuronal Activity Combined with Microiontophoretic Application of Neuroactive Substances in Awake Mice
dc.creator.none.fl_str_mv Aguilar Ayala, Yaneri
Pérez González, David
Duque Doncos, Daniel
Palmer, Alan R.
Malmierca, Manuel S.
author Aguilar Ayala, Yaneri
author_facet Aguilar Ayala, Yaneri
Pérez González, David
Duque Doncos, Daniel
Palmer, Alan R.
Malmierca, Manuel S.
author_role author
author2 Pérez González, David
Duque Doncos, Daniel
Palmer, Alan R.
Malmierca, Manuel S.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Auditory
Stimulus-specific adaptation
Inferior colliculus
Frequency response area
Gabazine
Inhibition
Multibarrels
Tungsten electrode
Synaptic inputs
Inferior Colliculi
2490 Neurociencias
2411.13 Fisiología de la Audición
colículos inferiores
topic Auditory
Stimulus-specific adaptation
Inferior colliculus
Frequency response area
Gabazine
Inhibition
Multibarrels
Tungsten electrode
Synaptic inputs
Inferior Colliculi
2490 Neurociencias
2411.13 Fisiología de la Audición
colículos inferiores
description Differences in the activity of neurotransmitters and neuromodulators, and consequently different neural responses, can be found between anesthetized and awake animals. Therefore, methods allowing the manipulation of synaptic systems in awake animals are required in order to determine the contribution of synaptic inputs to neuronal processing unaffected by anesthetics. Here, we present methodology for the construction of electrodes to simultaneously record extracellular neural activity and release multiple neuroactive substances at the vicinity of the recording sites in awake mice. By combining these procedures, we performed microiontophoretic injections of gabazine to selectively block GABAA receptors in neurons of the inferior colliculus of head-restrained mice. Gabazine successfully modified neural response properties such as the frequency response area and stimulus-specific adaptation. Thus, we demonstrate that our methods are suitable for recording single-unit activity and for dissecting the role of specific neurotransmitter receptors in auditory processing. The main limitation of the described procedure is the relatively short recording time (~3 hr), which is determined by the level of habituation of the animal to the recording sessions. On the other hand, multiple recording sessions can be performed in the same animal. The advantage of this technique over other experimental procedures used to manipulate the level of neurotransmission or neuromodulation (such as systemic injections or the use of optogenetic models), is that the drug effect is confined to the local synaptic inputs to the target neuron. In addition, the custom-manufacture of electrodes allows adjustment of specific parameters according to the neural structure and type of neuron of interest (such as the tip resistance for improving the signal-to-noise ratio of the recordings).
publishDate 2016
dc.date.none.fl_str_mv 2016
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10366/160458
url http://hdl.handle.net/10366/160458
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad - MINECO (BFU201343608-P)
Ministerio de Economía y Competitividad - MINECO (PSI2013-49348-EXP)
Junta de Castilla y León - JCYL (SA343U14)
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MyJove Corporation
publisher.none.fl_str_mv MyJove Corporation
dc.source.none.fl_str_mv reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca
instname:Universidad de Salamanca (USAL)
instname_str Universidad de Salamanca (USAL)
reponame_str GREDOS. Repositorio Institucional de la Universidad de Salamanca
collection GREDOS. Repositorio Institucional de la Universidad de Salamanca
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869420279293280256
score 15.81155

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