Chromatin immunoprecipitation and high throughput sequencing of SVCV-infected zebrafish reveals novel epigenetic histone methylation patterns involved in antiviral immune response

8 pages, 4 figures, 1 table

Detalles Bibliográficos
Autores: Medina-Gali, Regla María, Bello-Pérez, Melissa, Martínez-López, Alicia, Falcó, Alberto, Ortega-Villaizán, M. M., Encinar, José Antonio, Novoa, Beatriz, Coll Morales, Julio, Pérez, Luis
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2018
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/193354
Acceso en línea:http://hdl.handle.net/10261/193354
Access Level:acceso abierto
Palabra clave:Zebrafish
SVCV
Histone methylation
ChIP-seq
Innate immunity
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spelling Chromatin immunoprecipitation and high throughput sequencing of SVCV-infected zebrafish reveals novel epigenetic histone methylation patterns involved in antiviral immune responseMedina-Gali, Regla MaríaBello-Pérez, MelissaMartínez-López, AliciaFalcó, AlbertoOrtega-Villaizán, M. M.Encinar, José AntonioNovoa, BeatrizColl Morales, JulioPérez, LuisZebrafishSVCVHistone methylationChIP-seqInnate immunity8 pages, 4 figures, 1 tableChromatin immunoprecipitation (ChIP) and high throughput sequencing (ChIP-seq) have been used to assess histone methylation (epigenetic modification) dynamics within the internal organs of zebrafish after spring viremia of carp virus (SVCV) infection. Our results show H3K4me3 up-methylation in gene promoters associated with innate immune response during the first 5 days after SVCV infection. Gene Ontology (GO) enrichment analysis confirmed up-methylation in 218 genes in the “immune system process” category. In particular, the promoters of interferon (ifn), interferon stimulated genes (isg), Toll-like receptors (tlr) and c-reactive protein (crp) multi gene sets were marked with the permissive H3K4 methylation. Higher histone 3 methylation was associated with higher transcription levels of the corresponding genes. Therefore, the evidence presented here suggests that transcriptional regulation at the promoter level of key immune genes of the interferon signaling pathway and c-reactive proteins genes can be modulated by epigenetic modification of histones. This study emphasizes the importance of epigenetic control in the response of zebrafish to SVCV infectionThis study was supported by Spanish Ministerio de Educación y Competitividad Grant AGL2014-51773-C3 and grant BIO2017-82851-C3-1-R. Dr. Ortega-Villaizán is the holder of ERC Starting Grant 2014 GA639249. Melissa Belló-Pérez is the holder of GVAL fellowship ACIF/2016/207Peer reviewedElsevierMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192018info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/193354reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-51773-C3-2-Rhttps://doi.org/10.1016/j.fsi.2018.08.056Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1933542026-05-22T06:33:51Z
dc.title.none.fl_str_mv Chromatin immunoprecipitation and high throughput sequencing of SVCV-infected zebrafish reveals novel epigenetic histone methylation patterns involved in antiviral immune response
title Chromatin immunoprecipitation and high throughput sequencing of SVCV-infected zebrafish reveals novel epigenetic histone methylation patterns involved in antiviral immune response
spellingShingle Chromatin immunoprecipitation and high throughput sequencing of SVCV-infected zebrafish reveals novel epigenetic histone methylation patterns involved in antiviral immune response
Medina-Gali, Regla María
Zebrafish
SVCV
Histone methylation
ChIP-seq
Innate immunity
title_short Chromatin immunoprecipitation and high throughput sequencing of SVCV-infected zebrafish reveals novel epigenetic histone methylation patterns involved in antiviral immune response
title_full Chromatin immunoprecipitation and high throughput sequencing of SVCV-infected zebrafish reveals novel epigenetic histone methylation patterns involved in antiviral immune response
title_fullStr Chromatin immunoprecipitation and high throughput sequencing of SVCV-infected zebrafish reveals novel epigenetic histone methylation patterns involved in antiviral immune response
title_full_unstemmed Chromatin immunoprecipitation and high throughput sequencing of SVCV-infected zebrafish reveals novel epigenetic histone methylation patterns involved in antiviral immune response
title_sort Chromatin immunoprecipitation and high throughput sequencing of SVCV-infected zebrafish reveals novel epigenetic histone methylation patterns involved in antiviral immune response
dc.creator.none.fl_str_mv Medina-Gali, Regla María
Bello-Pérez, Melissa
Martínez-López, Alicia
Falcó, Alberto
Ortega-Villaizán, M. M.
Encinar, José Antonio
Novoa, Beatriz
Coll Morales, Julio
Pérez, Luis
author Medina-Gali, Regla María
author_facet Medina-Gali, Regla María
Bello-Pérez, Melissa
Martínez-López, Alicia
Falcó, Alberto
Ortega-Villaizán, M. M.
Encinar, José Antonio
Novoa, Beatriz
Coll Morales, Julio
Pérez, Luis
author_role author
author2 Bello-Pérez, Melissa
Martínez-López, Alicia
Falcó, Alberto
Ortega-Villaizán, M. M.
Encinar, José Antonio
Novoa, Beatriz
Coll Morales, Julio
Pérez, Luis
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Zebrafish
SVCV
Histone methylation
ChIP-seq
Innate immunity
topic Zebrafish
SVCV
Histone methylation
ChIP-seq
Innate immunity
description 8 pages, 4 figures, 1 table
publishDate 2018
dc.date.none.fl_str_mv 2018
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/193354
url http://hdl.handle.net/10261/193354
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-51773-C3-2-R
https://doi.org/10.1016/j.fsi.2018.08.056
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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