Comparative genomic analysis and expression of the APETALA2-like genes from barley, wheat, and barley-wheat amphiploids

13 pages, figures

Detalhes bibliográficos
Autores: Gil Humanes, Javier, Pistón, Fernando, Martín, Antonio, Barro Losada, Francisco
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2009
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/13976
Acesso em linha:http://hdl.handle.net/10261/13976
Access Level:acceso abierto
Palavra-chave:APETALA2-like
Arabidopsis
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spelling Comparative genomic analysis and expression of the APETALA2-like genes from barley, wheat, and barley-wheat amphiploidsGil Humanes, JavierPistón, FernandoMartín, AntonioBarro Losada, FranciscoAPETALA2-likeArabidopsis13 pages, figures[Background] The APETALA2-like genes form a large multi-gene family of transcription factors which play an important role during the plant life cycle, being key regulators of many developmental processes. Many studies in Arabidopsis have revealed that the APETALA2 (AP2) gene is implicated in the establishment of floral meristem and floral organ identity as well as temporal and spatial regulation of flower homeotic gene expression.[Results] In this work, we have cloned and characterised the AP2-like gene from accessions of Hordeum chilense and Hordeum vulgare, wild and domesticated barley, respectively, and compared with other AP2 homoeologous genes, including the Q gene in wheat. The Hordeum AP2-like genes contain two plant-specific DNA binding motifs called AP2 domains, as does the Q gene of wheat. We confirm that the H. chilense AP2-like gene is located on chromosome 5Hch. Patterns of expression of the AP2-like genes were examined in floral organs and other tissues in barley, wheat and in tritordeum amphiploids (barley × wheat hybrids). In tritordeum amphiploids, the level of transcription of the barley AP2-like gene was lower than in its barley parental and the chromosome substitutions 1D/1Hch and 2D/2Hch were seen to modify AP2 gene expression levels.[Conclusion] The results are of interest in order to understand the role of the AP2-like gene in the spike morphology of barley and wheat, and to understand the regulation of this gene in the amphiploids obtained from barley-wheat crossing. This information may have application in cereal breeding programs to up- or down-regulate the expression of AP2-like genes in order to modify spike characteristics and to obtain free-threshing plants.The authors acknowledge funding by the Spanish C.I.C.Y.T. (project AGL2007-65685-C02-01/AGR). Javier Gil-Humanes also acknowledges the financial support from the I3P Program from the C.S.I.C., co-financed by the European Social Fund. Technical assistance by Ana García is also acknowl-Peer reviewedBioMed Central200920092009info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersion1004632 bytesapplication/pdfhttp://hdl.handle.net/10261/13976reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://www.biomedcentral.com/1471-2229/9/66info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/139762026-05-22T06:33:51Z
dc.title.none.fl_str_mv Comparative genomic analysis and expression of the APETALA2-like genes from barley, wheat, and barley-wheat amphiploids
title Comparative genomic analysis and expression of the APETALA2-like genes from barley, wheat, and barley-wheat amphiploids
spellingShingle Comparative genomic analysis and expression of the APETALA2-like genes from barley, wheat, and barley-wheat amphiploids
Gil Humanes, Javier
APETALA2-like
Arabidopsis
title_short Comparative genomic analysis and expression of the APETALA2-like genes from barley, wheat, and barley-wheat amphiploids
title_full Comparative genomic analysis and expression of the APETALA2-like genes from barley, wheat, and barley-wheat amphiploids
title_fullStr Comparative genomic analysis and expression of the APETALA2-like genes from barley, wheat, and barley-wheat amphiploids
title_full_unstemmed Comparative genomic analysis and expression of the APETALA2-like genes from barley, wheat, and barley-wheat amphiploids
title_sort Comparative genomic analysis and expression of the APETALA2-like genes from barley, wheat, and barley-wheat amphiploids
dc.creator.none.fl_str_mv Gil Humanes, Javier
Pistón, Fernando
Martín, Antonio
Barro Losada, Francisco
author Gil Humanes, Javier
author_facet Gil Humanes, Javier
Pistón, Fernando
Martín, Antonio
Barro Losada, Francisco
author_role author
author2 Pistón, Fernando
Martín, Antonio
Barro Losada, Francisco
author2_role author
author
author
dc.subject.none.fl_str_mv APETALA2-like
Arabidopsis
topic APETALA2-like
Arabidopsis
description 13 pages, figures
publishDate 2009
dc.date.none.fl_str_mv 2009
2009
2009
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/13976
url http://hdl.handle.net/10261/13976
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://www.biomedcentral.com/1471-2229/9/66
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1004632 bytes
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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