Effect of the addition of exogenous progesterone and the progesterone receptor inhibitor (RU 486) on boar cryopreservation semen extenders

7 Pág.

Detalles Bibliográficos
Autores: Martín, Adrián, Gala, Nerea, Nieto-Cristóbal, Helena, Álvarez-Rodríguez, Manuel, de Mercado, Eduardo
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/367954
Acceso en línea:http://hdl.handle.net/10261/367954
https://api.elsevier.com/content/abstract/scopus_id/85202703075
Access Level:acceso abierto
Palabra clave:Boar
Cryocapacitation
Cryopreservation
Mifepristone
Progesterone
Sperm
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spelling Effect of the addition of exogenous progesterone and the progesterone receptor inhibitor (RU 486) on boar cryopreservation semen extendersMartín, AdriánGala, NereaNieto-Cristóbal, HelenaÁlvarez-Rodríguez, Manuelde Mercado, EduardoBoarCryocapacitationCryopreservationMifepristoneProgesteroneSperm7 Pág.Cryopreservation of porcine spermatozoa is detrimental due to their high sensitivity to cold shock, leading to changes akin to capacitation, known as cryocapacitation. These changes, including the acrosomal reaction, hypermotility induction, and protein phosphorylation, might be influenced by the presence of progesterone in seminal plasma and egg yolk, used in most freezing extenders. We tested the effect of various progesterone concentrations added to the freezing extenders (1, 10, and 100 μg/mL). At 100 μg/mL, progesterone decreased the proportion of straightness and tended to reduce viability and the proportion of progressive motility (p < 0.1). At 10 μg/mL, it increased reacted acrosomes in dead sperm (p < 0.05), protein phosphorylation rate (p < 0.05), and tended (p < 0.1) to enhance linear movement compared to the control. To counteract the capacitating effect of progesterone, we examined the effect of antiprogesterone mifepristone (RU 486) at concentrations of 5, 10, 20, 50, 100, and 200 μM, and co-incubated 10 μM of RU 486 with 10 μg/mL of progesterone. RU 486 maintained capacitation levels and motility parameters similar to the control, although high concentrations (100 μM) tended (p = 0.152) to increase protein phosphorylation. Co-incubation reduced the acrosome reaction in dead sperm, and RU 486 appeared to prevent hypermotility stabilizing motility and viability parameters compared to samples with progesterone alone. Protein phosphorylation increased and RU 486 could not restore capacitation to control levels due to its competitive antagonism for progesterone receptors, having less affinity than progesterone, which displaces RU 486 at high concentrations, allowing normal sperm capacitation.This research was funded by the projects PID2019-108320RJ–I00, RYC2020–028615-I, PID2022-136561OB-I00, and CNS2023–144564. funded by MCIN/AEI/10.13039/501100011033 (Spain) and FEDER, FSE and NextGenerationEU/PRTR funds (EU). N.G. developed part of the experimental approaches during an internship as a Final Degree Project (Universidad de Alcalá, Madrid, Spain).Peer reviewedElsevierAgencia Estatal de Investigación (España)Ministerio de Ciencia e Innovación (España)European CommissionUniversidad de AlcaláNieto-Cristóbal, Helena [0000-0003-1192-9467]Álvarez-Rodríguez, Manuel [0000-0003-0120-354X]de Mercado, Eduardo [0000-0003-0321-2431]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/367954https://api.elsevier.com/content/abstract/scopus_id/85202703075reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-108320RJ-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-136561OB-I00info:eu-repo/grantAgreement/MCIN//CNS2023–144564Departamento de Reproducción Animal​ https://doi.org/10.1016/j.rvsc.2024.105400Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3679542026-05-22T06:33:51Z
dc.title.none.fl_str_mv Effect of the addition of exogenous progesterone and the progesterone receptor inhibitor (RU 486) on boar cryopreservation semen extenders
title Effect of the addition of exogenous progesterone and the progesterone receptor inhibitor (RU 486) on boar cryopreservation semen extenders
spellingShingle Effect of the addition of exogenous progesterone and the progesterone receptor inhibitor (RU 486) on boar cryopreservation semen extenders
Martín, Adrián
Boar
Cryocapacitation
Cryopreservation
Mifepristone
Progesterone
Sperm
title_short Effect of the addition of exogenous progesterone and the progesterone receptor inhibitor (RU 486) on boar cryopreservation semen extenders
title_full Effect of the addition of exogenous progesterone and the progesterone receptor inhibitor (RU 486) on boar cryopreservation semen extenders
title_fullStr Effect of the addition of exogenous progesterone and the progesterone receptor inhibitor (RU 486) on boar cryopreservation semen extenders
title_full_unstemmed Effect of the addition of exogenous progesterone and the progesterone receptor inhibitor (RU 486) on boar cryopreservation semen extenders
title_sort Effect of the addition of exogenous progesterone and the progesterone receptor inhibitor (RU 486) on boar cryopreservation semen extenders
dc.creator.none.fl_str_mv Martín, Adrián
Gala, Nerea
Nieto-Cristóbal, Helena
Álvarez-Rodríguez, Manuel
de Mercado, Eduardo
author Martín, Adrián
author_facet Martín, Adrián
Gala, Nerea
Nieto-Cristóbal, Helena
Álvarez-Rodríguez, Manuel
de Mercado, Eduardo
author_role author
author2 Gala, Nerea
Nieto-Cristóbal, Helena
Álvarez-Rodríguez, Manuel
de Mercado, Eduardo
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación (España)
Ministerio de Ciencia e Innovación (España)
European Commission
Universidad de Alcalá
Nieto-Cristóbal, Helena [0000-0003-1192-9467]
Álvarez-Rodríguez, Manuel [0000-0003-0120-354X]
de Mercado, Eduardo [0000-0003-0321-2431]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Boar
Cryocapacitation
Cryopreservation
Mifepristone
Progesterone
Sperm
topic Boar
Cryocapacitation
Cryopreservation
Mifepristone
Progesterone
Sperm
description 7 Pág.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/367954
https://api.elsevier.com/content/abstract/scopus_id/85202703075
url http://hdl.handle.net/10261/367954
https://api.elsevier.com/content/abstract/scopus_id/85202703075
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
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info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-108320RJ-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-136561OB-I00
info:eu-repo/grantAgreement/MCIN//CNS2023–144564
Departamento de Reproducción Animal​ 
https://doi.org/10.1016/j.rvsc.2024.105400
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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