Breast milk immune composition varies during the transition stage of lactation: characterization of immunotypes in the MAMI cohort

<strong>Background:</strong> Breast milk is a complex and dynamic fluid needed for infant development and protection due to its content of bioactive factors such as immunoglobulins (Igs). Most studies focus primarily on IgA, but other types of Ig and even other immune components (cytokin...

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Autores: Rio Aige, Karla, Fernández Bargalló, Aina, Vegas Lozano, Esteban, Miñarro Alonso, Antonio, Castell, Margarida, Selma-Royo, Marta, Martínez Costa, Cecilia, Rodríguez Lagunas, María José, Collado, Maria Carmen, Pérez-Cano, Francisco J.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/219035
Acceso en línea:https://hdl.handle.net/2445/219035
Access Level:acceso abierto
Palabra clave:Immunoglobulines
Llet materna
Microbiota intestinal
Immunoglobulins
Breast milk
Gastrointestinal microbiome
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spelling Breast milk immune composition varies during the transition stage of lactation: characterization of immunotypes in the MAMI cohortRio Aige, KarlaFernández Bargalló, AinaVegas Lozano, EstebanMiñarro Alonso, AntonioCastell, MargaridaSelma-Royo, MartaMartínez Costa, CeciliaRodríguez Lagunas, María JoséCollado, Maria CarmenPérez-Cano, Francisco J.ImmunoglobulinesLlet maternaMicrobiota intestinalImmunoglobulinsBreast milkGastrointestinal microbiome<strong>Background:</strong> Breast milk is a complex and dynamic fluid needed for infant development and protection due to its content of bioactive factors such as immunoglobulins (Igs). Most studies focus primarily on IgA, but other types of Ig and even other immune components (cytokines and adipokines) may also play significant roles in neonatal health. As a first step, we aimed to characterize the Ig profile, many cytokines, and two adipokines (leptin and adiponectin) at two sampling time points within the transitional stage, which is the least studied phase in terms of these components. The secondary objective was to identify different breast milk immunotypes in the MAMI cohort substudy, and finally, we further aimed at analyzing maternal and infant characteristics to identify influencing factors of breast milk immune composition.<strong>Methods:</strong> Breast milk samples from 75 mothers were studied between days 7 and 15 postpartum. The Igs, cytokines, and adipokine levels were determined by a multiplex approach, except for the IgA, IgM, and leptin that were evaluated by ELISA.<strong>Results:</strong> IgA, IgM, IgE, IgG2, IL-1β, IL-5, IL-6, IL-10, and IL-17 were significantly higher on day 7 with respect to day 15. The multiple factor analysis (MFA) allowed us to identify two maternal clusters (immunotypes) depending on the breast milk immune profile evolution from day 7 to day 15, mainly due to the IgE and IgG subtypes, but not for IgA and IgM, which always presented higher levels early in time.<strong>Conclusion:</strong> All these results demonstrated the importance of the dynamics of the breast milk composition in terms of immune factors because even in the same lactation stage, a difference of 1 week has induced changes in the breast milk immune profile. Moreover, this immune profile does not evolve in the same way for all women. The dynamic compositional changes may be maternal-specific, as we observed differences in parity and exclusive breastfeeding between the two BM immunotype groups, which could potentially impact infant health.<br />Frontiers Media2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/219035Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3389/fnut.2023.1252815Frontiers In Nutrition, 2023, vol. 10, num.1252815https://doi.org/10.3389/fnut.2023.1252815cc-by (c) Rio-Aige, K. et al., 2023http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/2190352026-05-27T06:46:51Z
dc.title.none.fl_str_mv Breast milk immune composition varies during the transition stage of lactation: characterization of immunotypes in the MAMI cohort
title Breast milk immune composition varies during the transition stage of lactation: characterization of immunotypes in the MAMI cohort
spellingShingle Breast milk immune composition varies during the transition stage of lactation: characterization of immunotypes in the MAMI cohort
Rio Aige, Karla
Immunoglobulines
Llet materna
Microbiota intestinal
Immunoglobulins
Breast milk
Gastrointestinal microbiome
title_short Breast milk immune composition varies during the transition stage of lactation: characterization of immunotypes in the MAMI cohort
title_full Breast milk immune composition varies during the transition stage of lactation: characterization of immunotypes in the MAMI cohort
title_fullStr Breast milk immune composition varies during the transition stage of lactation: characterization of immunotypes in the MAMI cohort
title_full_unstemmed Breast milk immune composition varies during the transition stage of lactation: characterization of immunotypes in the MAMI cohort
title_sort Breast milk immune composition varies during the transition stage of lactation: characterization of immunotypes in the MAMI cohort
dc.creator.none.fl_str_mv Rio Aige, Karla
Fernández Bargalló, Aina
Vegas Lozano, Esteban
Miñarro Alonso, Antonio
Castell, Margarida
Selma-Royo, Marta
Martínez Costa, Cecilia
Rodríguez Lagunas, María José
Collado, Maria Carmen
Pérez-Cano, Francisco J.
author Rio Aige, Karla
author_facet Rio Aige, Karla
Fernández Bargalló, Aina
Vegas Lozano, Esteban
Miñarro Alonso, Antonio
Castell, Margarida
Selma-Royo, Marta
Martínez Costa, Cecilia
Rodríguez Lagunas, María José
Collado, Maria Carmen
Pérez-Cano, Francisco J.
author_role author
author2 Fernández Bargalló, Aina
Vegas Lozano, Esteban
Miñarro Alonso, Antonio
Castell, Margarida
Selma-Royo, Marta
Martínez Costa, Cecilia
Rodríguez Lagunas, María José
Collado, Maria Carmen
Pérez-Cano, Francisco J.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Immunoglobulines
Llet materna
Microbiota intestinal
Immunoglobulins
Breast milk
Gastrointestinal microbiome
topic Immunoglobulines
Llet materna
Microbiota intestinal
Immunoglobulins
Breast milk
Gastrointestinal microbiome
description <strong>Background:</strong> Breast milk is a complex and dynamic fluid needed for infant development and protection due to its content of bioactive factors such as immunoglobulins (Igs). Most studies focus primarily on IgA, but other types of Ig and even other immune components (cytokines and adipokines) may also play significant roles in neonatal health. As a first step, we aimed to characterize the Ig profile, many cytokines, and two adipokines (leptin and adiponectin) at two sampling time points within the transitional stage, which is the least studied phase in terms of these components. The secondary objective was to identify different breast milk immunotypes in the MAMI cohort substudy, and finally, we further aimed at analyzing maternal and infant characteristics to identify influencing factors of breast milk immune composition.<strong>Methods:</strong> Breast milk samples from 75 mothers were studied between days 7 and 15 postpartum. The Igs, cytokines, and adipokine levels were determined by a multiplex approach, except for the IgA, IgM, and leptin that were evaluated by ELISA.<strong>Results:</strong> IgA, IgM, IgE, IgG2, IL-1β, IL-5, IL-6, IL-10, and IL-17 were significantly higher on day 7 with respect to day 15. The multiple factor analysis (MFA) allowed us to identify two maternal clusters (immunotypes) depending on the breast milk immune profile evolution from day 7 to day 15, mainly due to the IgE and IgG subtypes, but not for IgA and IgM, which always presented higher levels early in time.<strong>Conclusion:</strong> All these results demonstrated the importance of the dynamics of the breast milk composition in terms of immune factors because even in the same lactation stage, a difference of 1 week has induced changes in the breast milk immune profile. Moreover, this immune profile does not evolve in the same way for all women. The dynamic compositional changes may be maternal-specific, as we observed differences in parity and exclusive breastfeeding between the two BM immunotype groups, which could potentially impact infant health.<br />
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/219035
url https://hdl.handle.net/2445/219035
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3389/fnut.2023.1252815
Frontiers In Nutrition, 2023, vol. 10, num.1252815
https://doi.org/10.3389/fnut.2023.1252815
dc.rights.none.fl_str_mv cc-by (c) Rio-Aige, K. et al., 2023
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Rio-Aige, K. et al., 2023
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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