Molecular and Phylogenetic Characterization of the Monkeypox Outbreak in the South of Spain

Background and Aim: Until the May 2022 Monkeypox (MPXV) outbreak, which spread rapidly to many non-endemic countries, the virus was considered a viral zoonosis limited to some African countries. The Andalusian circuit of genomic surveillance was rapidly applied to characterize the MPXV outbreak in t...

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Autores: Casimiro Soriguer, C. S., Pérez Florido, J., Lara, M., Camacho Martínez, P., Merino Díaz, L., Pupo Ledo, I., de Salazar, A., Fuentes, A., Vinuela, L., Chueca, N., Lepe Jiménez, José Antonio, Garcia, F.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/165849
Acceso en línea:https://hdl.handle.net/11441/165849
https://doi.org/10.1002/hsr2.1965
Access Level:acceso abierto
Palabra clave:Drug resistance
Genomic surveillance
Monkeypox
Outbreak
Whole genome sequencing
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spelling Molecular and Phylogenetic Characterization of the Monkeypox Outbreak in the South of SpainCasimiro Soriguer, C. S.Pérez Florido, J.Lara, M.Camacho Martínez, P.Merino Díaz, L.Pupo Ledo, I.de Salazar, A.Fuentes, A.Vinuela, L.Chueca, N.Lepe Jiménez, José AntonioGarcia, F.Drug resistanceGenomic surveillanceMonkeypoxOutbreakWhole genome sequencingBackground and Aim: Until the May 2022 Monkeypox (MPXV) outbreak, which spread rapidly to many non-endemic countries, the virus was considered a viral zoonosis limited to some African countries. The Andalusian circuit of genomic surveillance was rapidly applied to characterize the MPXV outbreak in the South of Spain. Methods: Whole genome sequencing was used to obtain the genomic profiles of samples collected across the south of Spain, representative of all the provinces of Andalusia. Phylogenetic analysis was used to study the relationship of the isolates and the available sequences of the 2022 outbreak. Results: Whole genome sequencing of a total of 160 MPXV viruses from the different provinces that reported cases were obtained. Interestingly, we report the sequences of MPXV viruses obtained from two patients who died. While one of the isolates bore no noteworthy mutations that explain a potential heightened virulence, in another patient the second consecutive genome sequence, performed after the administration of tecovirimat, uncovered a mutation within the A0A7H0DN30 gene, known to be a prime target for tecovirimat in its Vaccinia counterpart. In general, a low number of mutations were observed in the sequences reported, which were very similar to the reference of the 2022 outbreak (OX044336), as expected from a DNA virus. The samples likely correspond to several introductions of the circulating MPXV viruses from the last outbreak. The virus sequenced from one of the two patients that died presented a mutation in a gene that bears potential connections to drug resistance. This mutation was absent in the initial sequencing before treatment.Ministerio de Ciencia e Innovación PID2020‐117979RB‐I00, FJC2021‐046546‐IInstituto de Salud Carlos III IMP/00019John Wiley & SonsMicrobiologíaMinisterio de Ciencia e Innovación (MICIN). EspañaInstituto de Salud Carlos III2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/165849https://doi.org/10.1002/hsr2.1965reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésHealth Science Reports, 7 (3), e1965.PID2020‐117979RB‐I00FJC2021‐046546‐IIMP/00019https://doi.org/10.1002/hsr2.1965info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1658492026-06-17T12:51:07Z
dc.title.none.fl_str_mv Molecular and Phylogenetic Characterization of the Monkeypox Outbreak in the South of Spain
title Molecular and Phylogenetic Characterization of the Monkeypox Outbreak in the South of Spain
spellingShingle Molecular and Phylogenetic Characterization of the Monkeypox Outbreak in the South of Spain
Casimiro Soriguer, C. S.
Drug resistance
Genomic surveillance
Monkeypox
Outbreak
Whole genome sequencing
title_short Molecular and Phylogenetic Characterization of the Monkeypox Outbreak in the South of Spain
title_full Molecular and Phylogenetic Characterization of the Monkeypox Outbreak in the South of Spain
title_fullStr Molecular and Phylogenetic Characterization of the Monkeypox Outbreak in the South of Spain
title_full_unstemmed Molecular and Phylogenetic Characterization of the Monkeypox Outbreak in the South of Spain
title_sort Molecular and Phylogenetic Characterization of the Monkeypox Outbreak in the South of Spain
dc.creator.none.fl_str_mv Casimiro Soriguer, C. S.
Pérez Florido, J.
Lara, M.
Camacho Martínez, P.
Merino Díaz, L.
Pupo Ledo, I.
de Salazar, A.
Fuentes, A.
Vinuela, L.
Chueca, N.
Lepe Jiménez, José Antonio
Garcia, F.
author Casimiro Soriguer, C. S.
author_facet Casimiro Soriguer, C. S.
Pérez Florido, J.
Lara, M.
Camacho Martínez, P.
Merino Díaz, L.
Pupo Ledo, I.
de Salazar, A.
Fuentes, A.
Vinuela, L.
Chueca, N.
Lepe Jiménez, José Antonio
Garcia, F.
author_role author
author2 Pérez Florido, J.
Lara, M.
Camacho Martínez, P.
Merino Díaz, L.
Pupo Ledo, I.
de Salazar, A.
Fuentes, A.
Vinuela, L.
Chueca, N.
Lepe Jiménez, José Antonio
Garcia, F.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Microbiología
Ministerio de Ciencia e Innovación (MICIN). España
Instituto de Salud Carlos III
dc.subject.none.fl_str_mv Drug resistance
Genomic surveillance
Monkeypox
Outbreak
Whole genome sequencing
topic Drug resistance
Genomic surveillance
Monkeypox
Outbreak
Whole genome sequencing
description Background and Aim: Until the May 2022 Monkeypox (MPXV) outbreak, which spread rapidly to many non-endemic countries, the virus was considered a viral zoonosis limited to some African countries. The Andalusian circuit of genomic surveillance was rapidly applied to characterize the MPXV outbreak in the South of Spain. Methods: Whole genome sequencing was used to obtain the genomic profiles of samples collected across the south of Spain, representative of all the provinces of Andalusia. Phylogenetic analysis was used to study the relationship of the isolates and the available sequences of the 2022 outbreak. Results: Whole genome sequencing of a total of 160 MPXV viruses from the different provinces that reported cases were obtained. Interestingly, we report the sequences of MPXV viruses obtained from two patients who died. While one of the isolates bore no noteworthy mutations that explain a potential heightened virulence, in another patient the second consecutive genome sequence, performed after the administration of tecovirimat, uncovered a mutation within the A0A7H0DN30 gene, known to be a prime target for tecovirimat in its Vaccinia counterpart. In general, a low number of mutations were observed in the sequences reported, which were very similar to the reference of the 2022 outbreak (OX044336), as expected from a DNA virus. The samples likely correspond to several introductions of the circulating MPXV viruses from the last outbreak. The virus sequenced from one of the two patients that died presented a mutation in a gene that bears potential connections to drug resistance. This mutation was absent in the initial sequencing before treatment.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/165849
https://doi.org/10.1002/hsr2.1965
url https://hdl.handle.net/11441/165849
https://doi.org/10.1002/hsr2.1965
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Health Science Reports, 7 (3), e1965.
PID2020‐117979RB‐I00
FJC2021‐046546‐I
IMP/00019
https://doi.org/10.1002/hsr2.1965
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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