Essential role of Mg2+ in mouse preimplantation embryo development revealed by TRPM7 chanzyme-deficient gametes

[EN] TRPM7 (transient receptor potential cation channel subfamily M member 7) is a chanzyme with channel and kinase domains essential for embryo development. Using gamete-specific Trpm7-null lines, we report that TRPM7-mediated Mg2+ influx is indispensable for reaching the blastocyst stage. TRPM7 is...

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Autores: Grupta, Neha, Soriano Úbeda, Cristina de las Mercedes, Stein, Paula, Savy, Virginia, Papas, Brian N., Ardestani, Goli, Carvacho, Ingrid, Alfandari, Dominique, Williams, Carmen J., Fissore, Rafael A.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Universidad de León
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/24537
Acceso en línea:https://www.sciencedirect.com/science/article/pii/S2211124723012445?via%3Dihub
https://hdl.handle.net/10612/24537
Access Level:acceso abierto
Palabra clave:Veterinaria
MG2+
Mouses
TRPM7
3104.11 Reproducción
3109 Ciencias Veterinarias
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repository_id_str
spelling Essential role of Mg2+ in mouse preimplantation embryo development revealed by TRPM7 chanzyme-deficient gametesGrupta, NehaSoriano Úbeda, Cristina de las MercedesStein, PaulaSavy, VirginiaPapas, Brian N.Ardestani, GoliCarvacho, IngridAlfandari, DominiqueWilliams, Carmen J.Fissore, Rafael A.VeterinariaMG2+MousesTRPM73104.11 Reproducción3109 Ciencias Veterinarias[EN] TRPM7 (transient receptor potential cation channel subfamily M member 7) is a chanzyme with channel and kinase domains essential for embryo development. Using gamete-specific Trpm7-null lines, we report that TRPM7-mediated Mg2+ influx is indispensable for reaching the blastocyst stage. TRPM7 is expressed dynamically from gametes to blastocysts; displays stage-specific localization on the plasma membrane, cytoplasm, and nucleus; and undergoes cleavage that produces C-terminal kinase fragments. TRPM7 underpins Mg2+ homeostasis, and excess Mg2+ but not Zn2+ or Ca2+ overcomes the arrest of Trpm7-null embryos; expressing Trpm7 mRNA restores development, but mutant versions fail or are partially rescued. Transcriptomic analyses of Trpm7-null embryos reveal an abundance of oxidative stress-pathway genes, confirmed by mitochondrial dysfunction, and a reduction in transcription factor networks essential for proliferation; Mg2+ supplementation corrects these defects. Hence, TRPM7 underpins Mg2+ homeostasis in preimplantation embryos, prevents oxidative stress, and promotes gene expression patterns necessary for developmental progression and cell-lineage specificationSIWe thank Fissore lab members Dr. Hiroki Akizawa and Ms. Changli He for their valuable discussions and contributions in maintaining the mouse lines. We also thank Dr. Visconti’s lab for assistance with the analysis of sperm parameters using CASA. R.A.F. discloses support for this research from NIH (RO1 HD092499). C.J.W. discloses support for this research from the Intramural Research Program of the National Institutes of Health, National Institute of Environmental Health Sciences (1ZIAES102405). D.A. discloses support for this research from NIH R24 OD021485 and R01 DE016289. I.C.’s contributions were supported in part by the NIH (RO1 HD092499) and FONDECYT 1221308Cell PressMedicina y Cirugia AnimalFacultad de Veterinaria2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://www.sciencedirect.com/science/article/pii/S2211124723012445?via%3Dihubhttps://hdl.handle.net/10612/24537reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad de LeónIngléshttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/245372026-06-24T12:43:27Z
dc.title.none.fl_str_mv Essential role of Mg2+ in mouse preimplantation embryo development revealed by TRPM7 chanzyme-deficient gametes
title Essential role of Mg2+ in mouse preimplantation embryo development revealed by TRPM7 chanzyme-deficient gametes
spellingShingle Essential role of Mg2+ in mouse preimplantation embryo development revealed by TRPM7 chanzyme-deficient gametes
Grupta, Neha
Veterinaria
MG2+
Mouses
TRPM7
3104.11 Reproducción
3109 Ciencias Veterinarias
title_short Essential role of Mg2+ in mouse preimplantation embryo development revealed by TRPM7 chanzyme-deficient gametes
title_full Essential role of Mg2+ in mouse preimplantation embryo development revealed by TRPM7 chanzyme-deficient gametes
title_fullStr Essential role of Mg2+ in mouse preimplantation embryo development revealed by TRPM7 chanzyme-deficient gametes
title_full_unstemmed Essential role of Mg2+ in mouse preimplantation embryo development revealed by TRPM7 chanzyme-deficient gametes
title_sort Essential role of Mg2+ in mouse preimplantation embryo development revealed by TRPM7 chanzyme-deficient gametes
dc.creator.none.fl_str_mv Grupta, Neha
Soriano Úbeda, Cristina de las Mercedes
Stein, Paula
Savy, Virginia
Papas, Brian N.
Ardestani, Goli
Carvacho, Ingrid
Alfandari, Dominique
Williams, Carmen J.
Fissore, Rafael A.
author Grupta, Neha
author_facet Grupta, Neha
Soriano Úbeda, Cristina de las Mercedes
Stein, Paula
Savy, Virginia
Papas, Brian N.
Ardestani, Goli
Carvacho, Ingrid
Alfandari, Dominique
Williams, Carmen J.
Fissore, Rafael A.
author_role author
author2 Soriano Úbeda, Cristina de las Mercedes
Stein, Paula
Savy, Virginia
Papas, Brian N.
Ardestani, Goli
Carvacho, Ingrid
Alfandari, Dominique
Williams, Carmen J.
Fissore, Rafael A.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Medicina y Cirugia Animal
Facultad de Veterinaria
dc.subject.none.fl_str_mv Veterinaria
MG2+
Mouses
TRPM7
3104.11 Reproducción
3109 Ciencias Veterinarias
topic Veterinaria
MG2+
Mouses
TRPM7
3104.11 Reproducción
3109 Ciencias Veterinarias
description [EN] TRPM7 (transient receptor potential cation channel subfamily M member 7) is a chanzyme with channel and kinase domains essential for embryo development. Using gamete-specific Trpm7-null lines, we report that TRPM7-mediated Mg2+ influx is indispensable for reaching the blastocyst stage. TRPM7 is expressed dynamically from gametes to blastocysts; displays stage-specific localization on the plasma membrane, cytoplasm, and nucleus; and undergoes cleavage that produces C-terminal kinase fragments. TRPM7 underpins Mg2+ homeostasis, and excess Mg2+ but not Zn2+ or Ca2+ overcomes the arrest of Trpm7-null embryos; expressing Trpm7 mRNA restores development, but mutant versions fail or are partially rescued. Transcriptomic analyses of Trpm7-null embryos reveal an abundance of oxidative stress-pathway genes, confirmed by mitochondrial dysfunction, and a reduction in transcription factor networks essential for proliferation; Mg2+ supplementation corrects these defects. Hence, TRPM7 underpins Mg2+ homeostasis in preimplantation embryos, prevents oxidative stress, and promotes gene expression patterns necessary for developmental progression and cell-lineage specification
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S2211124723012445?via%3Dihub
https://hdl.handle.net/10612/24537
url https://www.sciencedirect.com/science/article/pii/S2211124723012445?via%3Dihub
https://hdl.handle.net/10612/24537
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Cell Press
publisher.none.fl_str_mv Cell Press
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad de León
instname_str Universidad de León
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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