Tracking bacterial virulence: global modulators as indicators

The genomes of Gram-negative bacteria encode paralogues and/or orthologues of global modulators. The nucleoid-associated H-NS and Hha proteins are an example: several enterobacteria such as Escherichia coli or Salmonella harbor H-NS, Hha and their corresponding paralogues, StpA and YdgT proteins, re...

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Autores: Prieto Durán, Alejandro, Urcola, Imanol, Blanco, Jorge, Dahbi, Ghizlane, Muniesa Pérez, Ma. Teresa, Quirós Fernández, Pablo, Falgenhauer, Linda, Chakraborty, Trinad, Hüttener Queiroz, Mário, Juárez Giménez, Antonio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/112612
Acceso en línea:https://hdl.handle.net/2445/112612
Access Level:acceso abierto
Palabra clave:Genètica microbiana
Microbiologia mèdica
Microbial genetics
Medical microbiology
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spelling Tracking bacterial virulence: global modulators as indicatorsPrieto Durán, AlejandroUrcola, ImanolBlanco, JorgeDahbi, GhizlaneMuniesa Pérez, Ma. TeresaQuirós Fernández, PabloFalgenhauer, LindaChakraborty, TrinadHüttener Queiroz, MárioJuárez Giménez, AntonioGenètica microbianaMicrobiologia mèdicaMicrobial geneticsMedical microbiologyThe genomes of Gram-negative bacteria encode paralogues and/or orthologues of global modulators. The nucleoid-associated H-NS and Hha proteins are an example: several enterobacteria such as Escherichia coli or Salmonella harbor H-NS, Hha and their corresponding paralogues, StpA and YdgT proteins, respectively. Remarkably, the genome of the pathogenic enteroaggregative E. coli strain 042 encodes, in addition to the hha and ydgT genes, two additional hha paralogues, hha2 and hha3. We show in this report that there exists a strong correlation between the presence of these paralogues and the virulence phenotype of several E. coli strains. hha2 and hha3 predominate in some groups of intestinal pathogenic E. coli strains (enteroaggregative and shiga toxin-producing isolates), as well as in the widely distributed extraintestinal ST131 isolates. Because of the relationship between the presence of hha2/hha3 and some virulence factors, we have been able to provide evidence for Hha2/Hha3 modulating the expression of the antigen 43 pathogenic determinants. We show that tracking global modulators or their paralogues/orthologues can be a new strategy to identify bacterial pathogenic clones and propose PCR amplification of hha2 and hha3 as a virulence indicator in environmental and clinical E. coli isolates.Nature Publishing Group2017201720162017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion11 p.application/pdfhttps://hdl.handle.net/2445/112612Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1038/srep25973Scientific Reports, 2016, vol. 6, p. 25973https://doi.org/10.1038/srep25973cc-by (c) Prieto, Alejandro et al., 2016http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1126122026-05-29T05:05:01Z
dc.title.none.fl_str_mv Tracking bacterial virulence: global modulators as indicators
title Tracking bacterial virulence: global modulators as indicators
spellingShingle Tracking bacterial virulence: global modulators as indicators
Prieto Durán, Alejandro
Genètica microbiana
Microbiologia mèdica
Microbial genetics
Medical microbiology
title_short Tracking bacterial virulence: global modulators as indicators
title_full Tracking bacterial virulence: global modulators as indicators
title_fullStr Tracking bacterial virulence: global modulators as indicators
title_full_unstemmed Tracking bacterial virulence: global modulators as indicators
title_sort Tracking bacterial virulence: global modulators as indicators
dc.creator.none.fl_str_mv Prieto Durán, Alejandro
Urcola, Imanol
Blanco, Jorge
Dahbi, Ghizlane
Muniesa Pérez, Ma. Teresa
Quirós Fernández, Pablo
Falgenhauer, Linda
Chakraborty, Trinad
Hüttener Queiroz, Mário
Juárez Giménez, Antonio
author Prieto Durán, Alejandro
author_facet Prieto Durán, Alejandro
Urcola, Imanol
Blanco, Jorge
Dahbi, Ghizlane
Muniesa Pérez, Ma. Teresa
Quirós Fernández, Pablo
Falgenhauer, Linda
Chakraborty, Trinad
Hüttener Queiroz, Mário
Juárez Giménez, Antonio
author_role author
author2 Urcola, Imanol
Blanco, Jorge
Dahbi, Ghizlane
Muniesa Pérez, Ma. Teresa
Quirós Fernández, Pablo
Falgenhauer, Linda
Chakraborty, Trinad
Hüttener Queiroz, Mário
Juárez Giménez, Antonio
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Genètica microbiana
Microbiologia mèdica
Microbial genetics
Medical microbiology
topic Genètica microbiana
Microbiologia mèdica
Microbial genetics
Medical microbiology
description The genomes of Gram-negative bacteria encode paralogues and/or orthologues of global modulators. The nucleoid-associated H-NS and Hha proteins are an example: several enterobacteria such as Escherichia coli or Salmonella harbor H-NS, Hha and their corresponding paralogues, StpA and YdgT proteins, respectively. Remarkably, the genome of the pathogenic enteroaggregative E. coli strain 042 encodes, in addition to the hha and ydgT genes, two additional hha paralogues, hha2 and hha3. We show in this report that there exists a strong correlation between the presence of these paralogues and the virulence phenotype of several E. coli strains. hha2 and hha3 predominate in some groups of intestinal pathogenic E. coli strains (enteroaggregative and shiga toxin-producing isolates), as well as in the widely distributed extraintestinal ST131 isolates. Because of the relationship between the presence of hha2/hha3 and some virulence factors, we have been able to provide evidence for Hha2/Hha3 modulating the expression of the antigen 43 pathogenic determinants. We show that tracking global modulators or their paralogues/orthologues can be a new strategy to identify bacterial pathogenic clones and propose PCR amplification of hha2 and hha3 as a virulence indicator in environmental and clinical E. coli isolates.
publishDate 2016
dc.date.none.fl_str_mv 2016
2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/112612
url https://hdl.handle.net/2445/112612
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1038/srep25973
Scientific Reports, 2016, vol. 6, p. 25973
https://doi.org/10.1038/srep25973
dc.rights.none.fl_str_mv cc-by (c) Prieto, Alejandro et al., 2016
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Prieto, Alejandro et al., 2016
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11 p.
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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