Supplementary material to the manuscript Ribosomal protein eL39 is important for maturation of the nascent polypeptide exit tunnel and proper protein folding during translation

Supplementary Table S1. Yeast strains used in this study. Supplementary Table S2. Plasmids used in this study. Supplementary Table S3. Oligonucleotides used in this study. Supplementary Figure S1. Pre-rRNA processing in S. cerevisiae. Supplementary Figure S2. The nascent polypeptide exit tunnel is c...

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Detalles Bibliográficos
Autores: Micic, Jelena, Rodríguez-Galán, Olga, Babiano, Reyes, Fitzgerald, Fiona, Fernández-Fernández, José, Zhang, Yunyang, Gao, Ning, Woolford, John L., Cruz, Jesús de la
Tipo de recurso: conjunto de datos
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/331679
Acceso en línea:http://hdl.handle.net/10261/331679
Access Level:acceso abierto
Descripción
Sumario:Supplementary Table S1. Yeast strains used in this study. Supplementary Table S2. Plasmids used in this study. Supplementary Table S3. Oligonucleotides used in this study. Supplementary Figure S1. Pre-rRNA processing in S. cerevisiae. Supplementary Figure S2. The nascent polypeptide exit tunnel is comprised of rRNA and three r-proteins uL4, uL22, and eL39. Supplementary Figure S3. The rpl39 D mutant exhibits a slow growth phenotype, enhanced at low temperatures. Supplementary Figure S4. eL39 is required for biogenesis of 60S r-subunits. Supplementary Figure S5. Wild-type and GFP-tagged eL39 complement the rpl39 D mutant. Supplementary Figure S6. The absence of eL39 leads to nuclear retention of pre-60S r-subunits, which is exaggerated in the cold. Supplementary Figure S7. Simplified pre-60S r-subunit assembly pathway. Supplementary Figure S8. Only partial densities for eL39 are visualized in the state E particles. Supplementary Figure S9. eL39 protein assembles in the nucleolus. Supplementary Figure S10. Accumulation of 27S and 7S pre-rRNAs in the absence of eL39 is not dependent on the presence of the TRAMP component Trf4 and the nuclear exosome component Rrp6. Supplementary Figure S11. Nog2-associated particles do not accumulate early assembling/early dissociating AFs in cells expressing rpf2 D255-344. References.

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