Adaptation to different salinities exposes functional specialization in the intestine of the sea bream (Sparus aurata L.)

© 2013. Published by The Company of Biologists Ltd.

Detalles Bibliográficos
Autores: Gregório, Silvia Filipa, Carvalho, Edison Samir M., Encarnação, Sandra, Wilson, Jonathan M., Power, Deborah M., Canario, Adelino V. M., Fuentes, Juan
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/345724
Acceso en línea:http://hdl.handle.net/10261/345724
Access Level:acceso abierto
Palabra clave:Marine fish
Salinity
Intestine
Ion transport
HCO3 – secretion
Water absorption
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spelling Adaptation to different salinities exposes functional specialization in the intestine of the sea bream (Sparus aurata L.)Gregório, Silvia FilipaCarvalho, Edison Samir M.Encarnação, SandraWilson, Jonathan M.Power, Deborah M.Canario, Adelino V. M.Fuentes, JuanMarine fishSalinityIntestineIon transportHCO3 – secretionWater absorption© 2013. Published by The Company of Biologists Ltd.The processing of intestinal fluid, in addition to a high drinking rate, is essential for osmoregulation in marine fish. This study analyzed the long-term response of the sea bream (Sparus aurata L.) to relevant changes of external salinity (12, 35 and 55 p.p.t.), focusing on the anterior intestine and in the less-often studied rectum. Intestinal water absorption, epithelial HCO3− secretion and gene expression of the main molecular mechanisms (SLC26a6, SLC26a3, SLC4a4, atp6v1b, CFTR, NKCC1 and NKCC2) involved in Cl− and HCO3− movements were examined. The anion transporters SLC26a6 and SLC26a3 are expressed severalfold higher in the anterior intestine, while the expression of Atp6v1b (V-type H+-ATPase β-subunit) is severalfold higher in the rectum. Prolonged exposure to altered external salinity was without effect on water absorption but was associated with concomitant changes in intestinal fluid content, epithelial HCO3− secretion and salinity-dependent expression of SLC26a6, SLC26a3 and SLC4a4 in the anterior intestine. However, the most striking response to external salinity was obtained in the rectum, where a 4- to 5-fold increase in water absorption was paralleled by a 2- to 3-fold increase in HCO3− secretion in response to a salinity of 55 p.p.t. In addition, the rectum of high salinity-acclimated fish shows a sustained (and enhanced) secretory current (Isc), identified in vitro in Ussing chambers and confirmed by the higher expression of CFTR and NKCC1 and by immunohistochemical protein localization. Taken together, the present results suggest a functional anterior–posterior specialization with regard to intestinal fluid processing and subsequently to salinity adaptation of the sea bream. The rectum becomes more active at higher salinities and functions as the final controller of intestinal function in osmoregulation.This work was supported by the Portuguese Foundation for Science and Technology (Ministry of Science and Higher Education, Portugal and European Social Funds) grant [PTDC/MAR/104008/2008] to J.F.Peer reviewedCompany of BiologistsFundação para a Ciência e a Tecnologia (Portugal)European CommissionFuentes, Juan [0000-0003-0430-8734]202420242013info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/345724reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1242/jeb.073742Noinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3457242026-05-22T06:33:51Z
dc.title.none.fl_str_mv Adaptation to different salinities exposes functional specialization in the intestine of the sea bream (Sparus aurata L.)
title Adaptation to different salinities exposes functional specialization in the intestine of the sea bream (Sparus aurata L.)
spellingShingle Adaptation to different salinities exposes functional specialization in the intestine of the sea bream (Sparus aurata L.)
Gregório, Silvia Filipa
Marine fish
Salinity
Intestine
Ion transport
HCO3 – secretion
Water absorption
title_short Adaptation to different salinities exposes functional specialization in the intestine of the sea bream (Sparus aurata L.)
title_full Adaptation to different salinities exposes functional specialization in the intestine of the sea bream (Sparus aurata L.)
title_fullStr Adaptation to different salinities exposes functional specialization in the intestine of the sea bream (Sparus aurata L.)
title_full_unstemmed Adaptation to different salinities exposes functional specialization in the intestine of the sea bream (Sparus aurata L.)
title_sort Adaptation to different salinities exposes functional specialization in the intestine of the sea bream (Sparus aurata L.)
dc.creator.none.fl_str_mv Gregório, Silvia Filipa
Carvalho, Edison Samir M.
Encarnação, Sandra
Wilson, Jonathan M.
Power, Deborah M.
Canario, Adelino V. M.
Fuentes, Juan
author Gregório, Silvia Filipa
author_facet Gregório, Silvia Filipa
Carvalho, Edison Samir M.
Encarnação, Sandra
Wilson, Jonathan M.
Power, Deborah M.
Canario, Adelino V. M.
Fuentes, Juan
author_role author
author2 Carvalho, Edison Samir M.
Encarnação, Sandra
Wilson, Jonathan M.
Power, Deborah M.
Canario, Adelino V. M.
Fuentes, Juan
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Fundação para a Ciência e a Tecnologia (Portugal)
European Commission
Fuentes, Juan [0000-0003-0430-8734]
dc.subject.none.fl_str_mv Marine fish
Salinity
Intestine
Ion transport
HCO3 – secretion
Water absorption
topic Marine fish
Salinity
Intestine
Ion transport
HCO3 – secretion
Water absorption
description © 2013. Published by The Company of Biologists Ltd.
publishDate 2013
dc.date.none.fl_str_mv 2013
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/345724
url http://hdl.handle.net/10261/345724
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1242/jeb.073742
No
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Company of Biologists
publisher.none.fl_str_mv Company of Biologists
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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