Pemphigus vulgaris autoantibodies induce apoptosis in HaCaT keratinocytes

Pemphigus vulgaris (PV) is an autoimmune disease characterized by binding of IgG autoantibodies to epidermal keratinocyte desmosomes. IgG autoantibodies obtained from a patient with mucocutaneous PV reacted with plakoglobin (Plkg) in addition to desmoglein-3 (Dsg3) and Dsg1. Immunofluorescence analy...

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Detalles Bibliográficos
Autores: Pelacho-Samper, B. (Beatriz)|||/items/aca35c0e-183d-435d-9d44-1888e0854ff3, Natal, C. (Cristina)|||/items/fc6a1b3c-b254-45f0-ae06-929d13e69be5, España-Alonso, A. (Agustín)|||/items/a6e3549c-ab30-4d2e-90a5-fd108573bc9f, Sanchez-Carpintero-Abad, I. (Ignacio)|||/items/f12ac303-7122-4b9b-9ccd-9e6ad5f48cc3, Iraburu-Elizalde, M. (María)|||/items/ce24b977-614c-4c59-9bc5-285acc790b73, Lopez-Zabalza, M.J. (María Jesús)|||/items/6b8ff4f5-9082-4027-8983-4482e8abdef3
Tipo de recurso: artículo
Fecha de publicación:2004
País:España
Institución:Universidad de Navarra
Repositorio:Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:inglés
OAI Identifier:oai:dadun.unav.edu:10171/27358
Acceso en línea:https://hdl.handle.net/10171/27358
Access Level:acceso abierto
Palabra clave:Autoantibodies/immunology/pharmacology
Cytoplasm/metabolism
Keratinocytes/cytology/immunology/metabolism/ultrastructure
Descripción
Sumario:Pemphigus vulgaris (PV) is an autoimmune disease characterized by binding of IgG autoantibodies to epidermal keratinocyte desmosomes. IgG autoantibodies obtained from a patient with mucocutaneous PV reacted with plakoglobin (Plkg) in addition to desmoglein-3 (Dsg3) and Dsg1. Immunofluorescence analysis confirmed that IgG autoantibodies, unlike antibodies from a healthy volunteer, caused disruption of cell-cell contacts in HaCaT keratinocytes. Moreover, apoptosis was enhanced in cells treated with autoantibodies compared to those treated with normal antibodies. The apoptotic process induced by IgG autoantibodies was characterized by caspase-3 activation, Bcl-2 depletion and Bax expression. The present report demonstrates that PV IgG autoantibodies promote apoptosis in HaCaT keratinocytes.

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