Histological assessment of a novel de-epithelialization method for connective tissue grafts harvested from the palate. An experimental study in cadavers

Objectives: This study aims to compare the histological outcomes of three distinct de-epithelialization methods used in (connective tissue grafts) CTG harvested from the palate. Materials and methods An experimental study using nine cadaver head specimens was carried out to compare 3 different de-ep...

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Autores: Bara Gaseni, Natalia, Jorba García, Adrià, Alberdi Navarro, Javier, Barbosa de Figueiredo, Rui Pedro, Bara Casaus, Javier
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2024
País:España
Recursos:Universidad de Barcelona
Repositório:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/214530
Acesso em linha:https://hdl.handle.net/2445/214530
Access Level:Acceso aberto
Palavra-chave:Histologia
Mucosa oral
Histology
Oral mucosa
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spelling Histological assessment of a novel de-epithelialization method for connective tissue grafts harvested from the palate. An experimental study in cadaversBara Gaseni, NataliaJorba García, AdriàAlberdi Navarro, JavierBarbosa de Figueiredo, Rui PedroBara Casaus, JavierHistologiaMucosa oralHistologyOral mucosaObjectives: This study aims to compare the histological outcomes of three distinct de-epithelialization methods used in (connective tissue grafts) CTG harvested from the palate. Materials and methods An experimental study using nine cadaver head specimens was carried out to compare 3 different de-epithelialization techniques for CTG. Eighteen samples were randomly allocated to three study groups: bone scraper, diamond bur and extraoral removal with a scalpel. The main outcome variable was the graft surface percentage without epithelium remains. Additionally, the time employed, and the graft thickness were also measured. Results Sixteen CTGs were analyzed. The extraoral scalpel group presented a total surface area with no epithelium of 58.84% (22.68) and a mean de-epithelialization time of 3.7 min; the intraoral diamond bur group had 88.24% (41.3) of the surface with no epithelium and took 1.455 min, and the intraoral bone scraper showed 97.98% (5.99) of surface without epithelium and a mean time of 0.815 min (P < 0.05). Histological analysis showed significant differences between the bone scraper and the extraoral group (P = 0.009). Conclusion The de-epithelialization technique with a bone scraper seems to be the most effective and fastest de-epithelialization technique for CTG. These findings need to be confirmed in future clinical studies with larger samples. Clinical relevance The use of bone scrapers, could be a simple, effective and fast technique to de-epithelialize connective tissue grafts harvested from the palatal area for both novice and experienced surgeons.Springer Science and Business Media LLC2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/214530Articles publicats en revistes (Odontoestomatologia)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1007/s00784-024-05734-yClinical Oral Investigations, 2024, vol. 28, num. 6https://doi.org/10.1007/s00784-024-05734-ycc-by (c) Bara Gaseni, Natalia et al., 2023http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/2145302026-05-27T06:46:51Z
dc.title.none.fl_str_mv Histological assessment of a novel de-epithelialization method for connective tissue grafts harvested from the palate. An experimental study in cadavers
title Histological assessment of a novel de-epithelialization method for connective tissue grafts harvested from the palate. An experimental study in cadavers
spellingShingle Histological assessment of a novel de-epithelialization method for connective tissue grafts harvested from the palate. An experimental study in cadavers
Bara Gaseni, Natalia
Histologia
Mucosa oral
Histology
Oral mucosa
title_short Histological assessment of a novel de-epithelialization method for connective tissue grafts harvested from the palate. An experimental study in cadavers
title_full Histological assessment of a novel de-epithelialization method for connective tissue grafts harvested from the palate. An experimental study in cadavers
title_fullStr Histological assessment of a novel de-epithelialization method for connective tissue grafts harvested from the palate. An experimental study in cadavers
title_full_unstemmed Histological assessment of a novel de-epithelialization method for connective tissue grafts harvested from the palate. An experimental study in cadavers
title_sort Histological assessment of a novel de-epithelialization method for connective tissue grafts harvested from the palate. An experimental study in cadavers
dc.creator.none.fl_str_mv Bara Gaseni, Natalia
Jorba García, Adrià
Alberdi Navarro, Javier
Barbosa de Figueiredo, Rui Pedro
Bara Casaus, Javier
author Bara Gaseni, Natalia
author_facet Bara Gaseni, Natalia
Jorba García, Adrià
Alberdi Navarro, Javier
Barbosa de Figueiredo, Rui Pedro
Bara Casaus, Javier
author_role author
author2 Jorba García, Adrià
Alberdi Navarro, Javier
Barbosa de Figueiredo, Rui Pedro
Bara Casaus, Javier
author2_role author
author
author
author
dc.subject.none.fl_str_mv Histologia
Mucosa oral
Histology
Oral mucosa
topic Histologia
Mucosa oral
Histology
Oral mucosa
description Objectives: This study aims to compare the histological outcomes of three distinct de-epithelialization methods used in (connective tissue grafts) CTG harvested from the palate. Materials and methods An experimental study using nine cadaver head specimens was carried out to compare 3 different de-epithelialization techniques for CTG. Eighteen samples were randomly allocated to three study groups: bone scraper, diamond bur and extraoral removal with a scalpel. The main outcome variable was the graft surface percentage without epithelium remains. Additionally, the time employed, and the graft thickness were also measured. Results Sixteen CTGs were analyzed. The extraoral scalpel group presented a total surface area with no epithelium of 58.84% (22.68) and a mean de-epithelialization time of 3.7 min; the intraoral diamond bur group had 88.24% (41.3) of the surface with no epithelium and took 1.455 min, and the intraoral bone scraper showed 97.98% (5.99) of surface without epithelium and a mean time of 0.815 min (P < 0.05). Histological analysis showed significant differences between the bone scraper and the extraoral group (P = 0.009). Conclusion The de-epithelialization technique with a bone scraper seems to be the most effective and fastest de-epithelialization technique for CTG. These findings need to be confirmed in future clinical studies with larger samples. Clinical relevance The use of bone scrapers, could be a simple, effective and fast technique to de-epithelialize connective tissue grafts harvested from the palatal area for both novice and experienced surgeons.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/214530
url https://hdl.handle.net/2445/214530
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1007/s00784-024-05734-y
Clinical Oral Investigations, 2024, vol. 28, num. 6
https://doi.org/10.1007/s00784-024-05734-y
dc.rights.none.fl_str_mv cc-by (c) Bara Gaseni, Natalia et al., 2023
http://creativecommons.org/licenses/by/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Bara Gaseni, Natalia et al., 2023
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Science and Business Media LLC
publisher.none.fl_str_mv Springer Science and Business Media LLC
dc.source.none.fl_str_mv Articles publicats en revistes (Odontoestomatologia)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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