Structural insights into nonsense-mediated mRNA decay (NMD) by electron microscopy

7 pag.; 3 figs.

Detalles Bibliográficos
Autor: Llorca, Óscar
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2013
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/121564
Acceso en línea:http://hdl.handle.net/10261/121564
Access Level:acceso abierto
Palabra clave:NMD
mRNA decay
SMG1
PIKK
UPF1
UPF2
UPF3
EJC
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spelling Structural insights into nonsense-mediated mRNA decay (NMD) by electron microscopyLlorca, ÓscarNMDmRNA decaySMG1PIKKUPF1UPF2UPF3EJC7 pag.; 3 figs.Nonsense-mediated mRNA decay (NMD) is a pathway that detects and degrades mRNAs containing premature translation termination codons (PTCs). In humans, recognition of these aberrant mRNAs requires an exon-junction-complex (EJC) placed downstream of a PTC and the dynamic interaction of several UPF/SMG proteins, the ribosome and the EJC. These interactions promote UPF1 phosphorylation by SMG1 kinase, triggering mRNA degradation. Recent progress in our understanding of NMD has been achieved by combining cryo-electron microscopy, high-resolution structures, interaction data and functional assays. These studies uncovered a mechanism regulating SMG1 kinase as well as the architecture and functional implications of a complex containing UPF1, UPF2, UPF3 and EJC. Collectively these findings reveal the role of protein scaffolds and conformational changes in NMD regulation.This work was funded by the Spanish Government (SAF2011-22988 to O.L.), the ‘Red Tematica de Investigacion Cooperativa en Cancer(RTICC)’ (RD06/0020/1001 to O.L.) and the Human Frontiers Science Program (RGP39/2008 to O.L.). O.L. acknowledges also support by the‘Ramon Areces’ Foundation.Peer reviewedElsevierMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201520152013info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/121564reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.sbi.2012.10.004Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1215642026-05-22T06:33:51Z
dc.title.none.fl_str_mv Structural insights into nonsense-mediated mRNA decay (NMD) by electron microscopy
title Structural insights into nonsense-mediated mRNA decay (NMD) by electron microscopy
spellingShingle Structural insights into nonsense-mediated mRNA decay (NMD) by electron microscopy
Llorca, Óscar
NMD
mRNA decay
SMG1
PIKK
UPF1
UPF2
UPF3
EJC
title_short Structural insights into nonsense-mediated mRNA decay (NMD) by electron microscopy
title_full Structural insights into nonsense-mediated mRNA decay (NMD) by electron microscopy
title_fullStr Structural insights into nonsense-mediated mRNA decay (NMD) by electron microscopy
title_full_unstemmed Structural insights into nonsense-mediated mRNA decay (NMD) by electron microscopy
title_sort Structural insights into nonsense-mediated mRNA decay (NMD) by electron microscopy
dc.creator.none.fl_str_mv Llorca, Óscar
author Llorca, Óscar
author_facet Llorca, Óscar
author_role author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv NMD
mRNA decay
SMG1
PIKK
UPF1
UPF2
UPF3
EJC
topic NMD
mRNA decay
SMG1
PIKK
UPF1
UPF2
UPF3
EJC
description 7 pag.; 3 figs.
publishDate 2013
dc.date.none.fl_str_mv 2013
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/121564
url http://hdl.handle.net/10261/121564
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.sbi.2012.10.004
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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