miRNA profiling in vitreous humor, vitreal exosomes and serum from uveal melanoma patients: Pathological and diagnostic implications
Uveal melanoma (UM) represents approximately 5–6% of all melanoma diagnoses and up to 50% of patients succumb to their disease. Although several methods are available, accurate diagnosis is not always easily feasible because of potential accidents (e.g., intraocular hemorrhage). Based on the assumpt...
| Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | article |
| Publication Date: | 2015 |
| Country: | España |
| Institution: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repository: | Dadun. Depósito Académico Digital de la Universidad de Navarra |
| Language: | English |
| OAI Identifier: | oai:dadun.unav.edu:10171/122264 |
| Online Access: | https://hdl.handle.net/10171/122264 |
| Access Level: | Open access |
| Keyword: | exosomes microRNAs serum uveal melanoma vitreous humor |
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miRNA profiling in vitreous humor, vitreal exosomes and serum from uveal melanoma patients: Pathological and diagnostic implicationsRagusa, M. (Marco)|||/items/246b6d9a-212c-4cb2-9b0e-262f72d4afd9Barbagallo, C. (Cristina)|||/items/8d6cf53e-d7be-4b9e-9d4d-cfbe281e6405Statello, L. (Luisa)|||/items/bd93ff38-fb37-46bd-9274-65da11af7f0fCaltabiano, R. (Rosario)|||/items/54e5d64d-c047-498e-b7ba-41f3049ca67bRusso, A.|||/items/6e8fa13f-97a5-4c34-a15f-40d539bb184aPuzzo, L. (Lidia)|||/items/9bdc0667-4675-43f1-8fa6-e80170e5831dAvitabile, T. (Teresio)|||/items/88acefcc-15a6-45bf-9124-1149f95aa669Longo, A. (Antonio)|||/items/f12ed5e0-0f7c-4c69-a594-17013688888eToro, M.D. (Mario D.)|||/items/ded9aadd-b4d6-4913-8f2b-b26dcb111ef4Barbagallo, D. (Davide)|||/items/1e0ce3cf-058e-4e0f-9085-5ff7f325ebd6Valadi, H. (Hadi)|||/items/f880a69d-c00a-4515-8e54-d6e537730c79Di Pietro, C. (Cinzia)|||/items/d6067d86-5317-40a8-bd69-63788773bbddPurrello, M. (Michele)|||/items/32d3ee1f-a04f-4d03-88a0-24f52ba7a1d2Reibaldi, M. (Michele)|||/items/1dd3d07f-4dd8-4afb-b0d8-99a56c540bb3exosomesmicroRNAsserumuveal melanomavitreous humorUveal melanoma (UM) represents approximately 5–6% of all melanoma diagnoses and up to 50% of patients succumb to their disease. Although several methods are available, accurate diagnosis is not always easily feasible because of potential accidents (e.g., intraocular hemorrhage). Based on the assumption that the profile of circulating miRNAs is often altered in human cancers, we verified whether UM patients showed different vitreous humor (VH) or serum miRNA profiles with respect to healthy controls. By using TaqMan Low Density Arrays, we analyzed 754 miRNAs from VH, vitreal exosomes, and serum of 6 UM patients and 6 healthy donors: our data demonstrated that the UM VH profile was unique and only partially overlapping with that from serum of the same patients. Whereas, 90% of miRNAs were shared between VH and vitreal exosomes, and their alterations in UM were statistically overlapped with those of VH and vitreal exosomes, suggesting that VH alterations could result from exosomal dysregulation. We report 32 miRNAs differentially expressed in UM patients in at least 2 different types of samples analyzed. We validated these data on an independent cohort of 12 UM patients. Most alterations were common to VH and vitreal exosomes (e.g., upregulation of miR-21,-34 a,-146a). Interestingly, miR-146a was upregulated in the serum of UM patients, as well as in serum exosomes. Upregulation of miR-21 and miR-146a was also detected in formalin-fixed, paraffin-embedded UM, suggesting that VH or serum alterations in UM could be the consequence of disregulation arising from tumoral cells. Our findings suggest the possibility to detect in VH and serum of UM patients “diagnostic” miRNAs released by the affected eye: based on this, miR-146a could be considered a potential circulating marker of UM.Taylor & FrancisDadun. Depósito Académico Digital Universidad de Navarra20152015-01-0120152015-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/122264reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/1222642026-06-21T12:47:57Z |
| dc.title.none.fl_str_mv |
miRNA profiling in vitreous humor, vitreal exosomes and serum from uveal melanoma patients: Pathological and diagnostic implications |
| title |
miRNA profiling in vitreous humor, vitreal exosomes and serum from uveal melanoma patients: Pathological and diagnostic implications |
| spellingShingle |
miRNA profiling in vitreous humor, vitreal exosomes and serum from uveal melanoma patients: Pathological and diagnostic implications Ragusa, M. (Marco)|||/items/246b6d9a-212c-4cb2-9b0e-262f72d4afd9 exosomes microRNAs serum uveal melanoma vitreous humor |
| title_short |
miRNA profiling in vitreous humor, vitreal exosomes and serum from uveal melanoma patients: Pathological and diagnostic implications |
| title_full |
miRNA profiling in vitreous humor, vitreal exosomes and serum from uveal melanoma patients: Pathological and diagnostic implications |
| title_fullStr |
miRNA profiling in vitreous humor, vitreal exosomes and serum from uveal melanoma patients: Pathological and diagnostic implications |
| title_full_unstemmed |
miRNA profiling in vitreous humor, vitreal exosomes and serum from uveal melanoma patients: Pathological and diagnostic implications |
| title_sort |
miRNA profiling in vitreous humor, vitreal exosomes and serum from uveal melanoma patients: Pathological and diagnostic implications |
| dc.creator.none.fl_str_mv |
Ragusa, M. (Marco)|||/items/246b6d9a-212c-4cb2-9b0e-262f72d4afd9 Barbagallo, C. (Cristina)|||/items/8d6cf53e-d7be-4b9e-9d4d-cfbe281e6405 Statello, L. (Luisa)|||/items/bd93ff38-fb37-46bd-9274-65da11af7f0f Caltabiano, R. (Rosario)|||/items/54e5d64d-c047-498e-b7ba-41f3049ca67b Russo, A.|||/items/6e8fa13f-97a5-4c34-a15f-40d539bb184a Puzzo, L. (Lidia)|||/items/9bdc0667-4675-43f1-8fa6-e80170e5831d Avitabile, T. (Teresio)|||/items/88acefcc-15a6-45bf-9124-1149f95aa669 Longo, A. (Antonio)|||/items/f12ed5e0-0f7c-4c69-a594-17013688888e Toro, M.D. (Mario D.)|||/items/ded9aadd-b4d6-4913-8f2b-b26dcb111ef4 Barbagallo, D. (Davide)|||/items/1e0ce3cf-058e-4e0f-9085-5ff7f325ebd6 Valadi, H. (Hadi)|||/items/f880a69d-c00a-4515-8e54-d6e537730c79 Di Pietro, C. (Cinzia)|||/items/d6067d86-5317-40a8-bd69-63788773bbdd Purrello, M. (Michele)|||/items/32d3ee1f-a04f-4d03-88a0-24f52ba7a1d2 Reibaldi, M. (Michele)|||/items/1dd3d07f-4dd8-4afb-b0d8-99a56c540bb3 |
| author |
Ragusa, M. (Marco)|||/items/246b6d9a-212c-4cb2-9b0e-262f72d4afd9 |
| author_facet |
Ragusa, M. (Marco)|||/items/246b6d9a-212c-4cb2-9b0e-262f72d4afd9 Barbagallo, C. (Cristina)|||/items/8d6cf53e-d7be-4b9e-9d4d-cfbe281e6405 Statello, L. (Luisa)|||/items/bd93ff38-fb37-46bd-9274-65da11af7f0f Caltabiano, R. (Rosario)|||/items/54e5d64d-c047-498e-b7ba-41f3049ca67b Russo, A.|||/items/6e8fa13f-97a5-4c34-a15f-40d539bb184a Puzzo, L. (Lidia)|||/items/9bdc0667-4675-43f1-8fa6-e80170e5831d Avitabile, T. (Teresio)|||/items/88acefcc-15a6-45bf-9124-1149f95aa669 Longo, A. (Antonio)|||/items/f12ed5e0-0f7c-4c69-a594-17013688888e Toro, M.D. (Mario D.)|||/items/ded9aadd-b4d6-4913-8f2b-b26dcb111ef4 Barbagallo, D. (Davide)|||/items/1e0ce3cf-058e-4e0f-9085-5ff7f325ebd6 Valadi, H. (Hadi)|||/items/f880a69d-c00a-4515-8e54-d6e537730c79 Di Pietro, C. (Cinzia)|||/items/d6067d86-5317-40a8-bd69-63788773bbdd Purrello, M. (Michele)|||/items/32d3ee1f-a04f-4d03-88a0-24f52ba7a1d2 Reibaldi, M. (Michele)|||/items/1dd3d07f-4dd8-4afb-b0d8-99a56c540bb3 |
| author_role |
author |
| author2 |
Barbagallo, C. (Cristina)|||/items/8d6cf53e-d7be-4b9e-9d4d-cfbe281e6405 Statello, L. (Luisa)|||/items/bd93ff38-fb37-46bd-9274-65da11af7f0f Caltabiano, R. (Rosario)|||/items/54e5d64d-c047-498e-b7ba-41f3049ca67b Russo, A.|||/items/6e8fa13f-97a5-4c34-a15f-40d539bb184a Puzzo, L. (Lidia)|||/items/9bdc0667-4675-43f1-8fa6-e80170e5831d Avitabile, T. (Teresio)|||/items/88acefcc-15a6-45bf-9124-1149f95aa669 Longo, A. (Antonio)|||/items/f12ed5e0-0f7c-4c69-a594-17013688888e Toro, M.D. (Mario D.)|||/items/ded9aadd-b4d6-4913-8f2b-b26dcb111ef4 Barbagallo, D. (Davide)|||/items/1e0ce3cf-058e-4e0f-9085-5ff7f325ebd6 Valadi, H. (Hadi)|||/items/f880a69d-c00a-4515-8e54-d6e537730c79 Di Pietro, C. (Cinzia)|||/items/d6067d86-5317-40a8-bd69-63788773bbdd Purrello, M. (Michele)|||/items/32d3ee1f-a04f-4d03-88a0-24f52ba7a1d2 Reibaldi, M. (Michele)|||/items/1dd3d07f-4dd8-4afb-b0d8-99a56c540bb3 |
| author2_role |
author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Dadun. Depósito Académico Digital Universidad de Navarra |
| dc.subject.none.fl_str_mv |
exosomes microRNAs serum uveal melanoma vitreous humor |
| topic |
exosomes microRNAs serum uveal melanoma vitreous humor |
| description |
Uveal melanoma (UM) represents approximately 5–6% of all melanoma diagnoses and up to 50% of patients succumb to their disease. Although several methods are available, accurate diagnosis is not always easily feasible because of potential accidents (e.g., intraocular hemorrhage). Based on the assumption that the profile of circulating miRNAs is often altered in human cancers, we verified whether UM patients showed different vitreous humor (VH) or serum miRNA profiles with respect to healthy controls. By using TaqMan Low Density Arrays, we analyzed 754 miRNAs from VH, vitreal exosomes, and serum of 6 UM patients and 6 healthy donors: our data demonstrated that the UM VH profile was unique and only partially overlapping with that from serum of the same patients. Whereas, 90% of miRNAs were shared between VH and vitreal exosomes, and their alterations in UM were statistically overlapped with those of VH and vitreal exosomes, suggesting that VH alterations could result from exosomal dysregulation. We report 32 miRNAs differentially expressed in UM patients in at least 2 different types of samples analyzed. We validated these data on an independent cohort of 12 UM patients. Most alterations were common to VH and vitreal exosomes (e.g., upregulation of miR-21,-34 a,-146a). Interestingly, miR-146a was upregulated in the serum of UM patients, as well as in serum exosomes. Upregulation of miR-21 and miR-146a was also detected in formalin-fixed, paraffin-embedded UM, suggesting that VH or serum alterations in UM could be the consequence of disregulation arising from tumoral cells. Our findings suggest the possibility to detect in VH and serum of UM patients “diagnostic” miRNAs released by the affected eye: based on this, miR-146a could be considered a potential circulating marker of UM. |
| publishDate |
2015 |
| dc.date.none.fl_str_mv |
2015 2015-01-01 2015 2015-01-01 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/10171/122264 |
| url |
https://hdl.handle.net/10171/122264 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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application/pdf |
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Taylor & Francis |
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Taylor & Francis |
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reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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Consejo Superior de Investigaciones Científicas (CSIC) |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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15,812429 |