Novel Mycoviruses Discovered in the Mycovirome of a Necrotrophic Fungus
Centro de Biotecnología y Genómica de Plantas (CBGP)
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/268477 |
| Acceso en línea: | http://hdl.handle.net/10261/268477 https://api.elsevier.com/content/abstract/scopus_id/85105662017 |
| Access Level: | acceso abierto |
| Palabra clave: | Botrytis cinerea Binarnavirus Fungi Grapevine Mycovirome Plant pathogens ssDNA mycovirus Trisegmented ssRNA– virus Virome Virus metagenomics |
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Novel Mycoviruses Discovered in the Mycovirome of a Necrotrophic Fungus |
| title |
Novel Mycoviruses Discovered in the Mycovirome of a Necrotrophic Fungus |
| spellingShingle |
Novel Mycoviruses Discovered in the Mycovirome of a Necrotrophic Fungus Ruiz-Padilla, Ana Botrytis cinerea Binarnavirus Fungi Grapevine Mycovirome Plant pathogens ssDNA mycovirus Trisegmented ssRNA– virus Virome Virus metagenomics |
| title_short |
Novel Mycoviruses Discovered in the Mycovirome of a Necrotrophic Fungus |
| title_full |
Novel Mycoviruses Discovered in the Mycovirome of a Necrotrophic Fungus |
| title_fullStr |
Novel Mycoviruses Discovered in the Mycovirome of a Necrotrophic Fungus |
| title_full_unstemmed |
Novel Mycoviruses Discovered in the Mycovirome of a Necrotrophic Fungus |
| title_sort |
Novel Mycoviruses Discovered in the Mycovirome of a Necrotrophic Fungus |
| dc.creator.none.fl_str_mv |
Ruiz-Padilla, Ana Rodríguez-Romero, Julio Gómez-Cid, Irene Pacifico, Davide Ayllón, María A. |
| author |
Ruiz-Padilla, Ana |
| author_facet |
Ruiz-Padilla, Ana Rodríguez-Romero, Julio Gómez-Cid, Irene Pacifico, Davide Ayllón, María A. |
| author_role |
author |
| author2 |
Rodríguez-Romero, Julio Gómez-Cid, Irene Pacifico, Davide Ayllón, María A. |
| author2_role |
author author author author |
| dc.contributor.none.fl_str_mv |
Agencia Estatal de Investigación (España) Comunidad de Madrid European Commission Ruiz-Padilla, Ana [0000-0001-9165-2845] Rodríguez-Romero, Julio [0000-0001-8304-4014] Pacifico, Davide [0000-0003-3620-9829] Ayllón, María A [0000-0001-7893-6106] Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
Botrytis cinerea Binarnavirus Fungi Grapevine Mycovirome Plant pathogens ssDNA mycovirus Trisegmented ssRNA– virus Virome Virus metagenomics |
| topic |
Botrytis cinerea Binarnavirus Fungi Grapevine Mycovirome Plant pathogens ssDNA mycovirus Trisegmented ssRNA– virus Virome Virus metagenomics |
| description |
Centro de Biotecnología y Genómica de Plantas (CBGP) |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021 2022 2022 |
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info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Publisher's version info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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http://hdl.handle.net/10261/268477 https://api.elsevier.com/content/abstract/scopus_id/85105662017 |
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http://hdl.handle.net/10261/268477 https://api.elsevier.com/content/abstract/scopus_id/85105662017 |
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Inglés |
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Inglés |
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info:eu-repo/semantics/openAccess |
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openAccess |
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American Society for Microbiology |
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American Society for Microbiology |
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reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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Consejo Superior de Investigaciones Científicas (CSIC) |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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1869420167670267904 |
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Novel Mycoviruses Discovered in the Mycovirome of a Necrotrophic FungusRuiz-Padilla, AnaRodríguez-Romero, JulioGómez-Cid, IrenePacifico, DavideAyllón, María A.Botrytis cinereaBinarnavirusFungiGrapevineMycoviromePlant pathogensssDNA mycovirusTrisegmented ssRNA– virusViromeVirus metagenomicsCentro de Biotecnología y Genómica de Plantas (CBGP)Botrytis cinerea is one of the most important plant-pathogenic fungus. Products based on microorganisms can be used in biocontrol strategies alternative to chemical control, and mycoviruses have been explored as putative biological agents in such approaches. Here, we have explored the mycovirome of B. cinerea isolates from grapevine of Italy and Spain to increase the knowledge about mycoviral diversity and evolution, and to search for new widely distributed mycoviruses that could be active ingredients in biological products to control this hazardous fungus. A total of 248 B. cinerea field isolates were used for our metatranscriptomic study. Ninety-two mycoviruses were identified: 62 new mycoviral species constituting putative novel viral genera and families. Of these mycoviruses, 57 had a positive-sense single-stranded RNA (ssRNA) genome, 19 contained a double-stranded RNA (dsRNA) genome, 15 had a negative-sense ssRNA genome, and 1 contained a single-stranded DNA (ssDNA) genome. In general, ssRNA mycoviruses were widely distributed in all sampled regions, the ssDNA mycovirus was more frequently found in Spain, and dsRNA mycoviruses were scattered in some pools of both countries. Some of the identified mycoviruses belong to clades that have never been found associated with Botrytis species: Botrytis-infecting narnaviruses; alpha-like, umbra-like, and tymo-like ssRNA+ mycoviruses; trisegmented ssRNA- mycovirus; bisegmented and tetrasegmented dsRNA mycoviruses; and finally, an ssDNA mycovirus. Among the results obtained in this massive mycovirus screening, the discovery of novel bisegmented viruses, phylogenetically related to narnaviruses, is remarkable.IMPORTANCE The results obtained here have expanded our knowledge of mycoviral diversity, horizontal transfers, and putative cross-kingdom events. To date, this study presents the most extensive and wide diversity collection of mycoviruses infecting the necrotrophic fungus B. cinerea The collection included all types of mycoviruses, with dsRNA, ssRNA+, ssRNA-, and ssDNA genomes, most of which were discovered here, and some of which were previously reported as infecting B. cinerea or other plant-pathogenic fungi. Some of these mycoviruses are reported for the first time here associated with B. cinerea, as a trisegmented ssRNA- mycovirus and as an ssDNA mycovirus, but even more remarkablly, we also describe here four novel bisegmented viruses (binarnaviruses) not previously described in nature. The present findings significantly contribute to general knowledge in virology and more particularly in the field of mycovirology.J.R.-R. was partially supported by a postdoctoral fellowship from the Severo Ochoa Program for Centers of Excellence in R&D (2017–2021) from the Agencia Estatal de Investigación of Spain (grant SEV-2016-0672 to the Centro de Biotecnología y Genómica de Plantas). I.G.-C. was supported by contract PEJ-2018-TL/BIO-11747 from Comunidad de Madrid, Spain. This study was completely supported financially by VIROPLANT, a project that received funding from the European Union’s Horizon 2020 Research and Innovation Program (grant agreement number 773567)Peer reviewedAmerican Society for MicrobiologyAgencia Estatal de Investigación (España)Comunidad de MadridEuropean CommissionRuiz-Padilla, Ana [0000-0001-9165-2845]Rodríguez-Romero, Julio [0000-0001-8304-4014]Pacifico, Davide [0000-0003-3620-9829]Ayllón, María A [0000-0001-7893-6106]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/268477https://api.elsevier.com/content/abstract/scopus_id/85105662017reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/SEV-2016-0672 to the Centro de Biotecnología y Genómica de Plantas)/PEJ-2018-TL/BIO-11747info:eu-repo/grantAgreement/EC/H2020/773567mBiohttps://doi.org/10.1128/mBio.03705-20Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2684772026-05-22T06:33:51Z |
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15,81155 |