A Novel non-invasive optical framework for simultaneous analysis of contractility and calcium in single-cell cardiomyocytes.

The use of a video method based on the Digital Image Correlation (DIC) algorithm from experimental mechanics to estimate the displacements, strain field, and sarcolemma length in a beating single-cell cardiomyocyte is proposed in this work. The obtained deformation is then correlated with the calciu...

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Autores: Marimon X, Esquinas F, Ferrer M, Cerrolaza M, Portela A, Benítez R
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Recursos:Fundació Sant Joan de Déu
Repositorio:r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
OAI Identifier:oai:fsjd.fundanetsuite.com:p27209
Acesso em linha:https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=27209
Access Level:acceso abierto
Palavra-chave:Calcium imaging
Cardiomyocyte
Cellular biomechanics
Continuum mechanics
Digital image correlation
Image processing
Strain analysis
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spelling A Novel non-invasive optical framework for simultaneous analysis of contractility and calcium in single-cell cardiomyocytes.Marimon XEsquinas FFerrer MCerrolaza MPortela ABenítez RCalcium imagingCardiomyocyteCellular biomechanicsContinuum mechanicsDigital image correlationImage processingStrain analysisThe use of a video method based on the Digital Image Correlation (DIC) algorithm from experimental mechanics to estimate the displacements, strain field, and sarcolemma length in a beating single-cell cardiomyocyte is proposed in this work. The obtained deformation is then correlated with the calcium signal, from calcium imaging where fluorescent dyes sensitive to calcium Ca(2+) are used. Our proposed video-based method for simultaneous contraction and intracellular calcium analysis results in a low-cost, non-invasive, and label-free method. This technique has shown great advantages in long-term observations because this type of intervention-free measurement neutralizes the possible alteration in the beating cardiomyocyte introduced by other techniques for measuring cell contractility (e.g., Traction Force Microscopy, Atomic Force Microscopy, Microfabrication or Optical tweezers). Three tests were performed with synthetically augmented data from cardiomyocyte images to validate the robustness of the algorithm. First, a simulated rigid translation of a referenced image is applied, then a rotation, and finally a controlled longitudinal deformation of the referenced image, thus simulating a native realistic deformation. Finally, the proposed framework is evaluated with real experimental data. To validate contraction induced by intracellular calcium concentration, this signal is correlated with a new deformation measure proposed in this article, which is independent of cell orientation in the imaging setup. Finally, based on the displacements obtained by the DIC algorithm, the change in sarcolemma length in a contracting cardiomyocyte is calculated and its temporal correlation with the calcium signal is obtained.ELSEVIER2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=27209Journal of the Mechanical Behavior of Biomedical MaterialsISSN: 17516161ISSNe: 18780180reponame:r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déuinstname:Fundació Sant Joan de DéuInglésinfo:eu-repo/semantics/openAccessoai:fsjd.fundanetsuite.com:p272092026-05-27T12:37:41Z
dc.title.none.fl_str_mv A Novel non-invasive optical framework for simultaneous analysis of contractility and calcium in single-cell cardiomyocytes.
title A Novel non-invasive optical framework for simultaneous analysis of contractility and calcium in single-cell cardiomyocytes.
spellingShingle A Novel non-invasive optical framework for simultaneous analysis of contractility and calcium in single-cell cardiomyocytes.
Marimon X
Calcium imaging
Cardiomyocyte
Cellular biomechanics
Continuum mechanics
Digital image correlation
Image processing
Strain analysis
title_short A Novel non-invasive optical framework for simultaneous analysis of contractility and calcium in single-cell cardiomyocytes.
title_full A Novel non-invasive optical framework for simultaneous analysis of contractility and calcium in single-cell cardiomyocytes.
title_fullStr A Novel non-invasive optical framework for simultaneous analysis of contractility and calcium in single-cell cardiomyocytes.
title_full_unstemmed A Novel non-invasive optical framework for simultaneous analysis of contractility and calcium in single-cell cardiomyocytes.
title_sort A Novel non-invasive optical framework for simultaneous analysis of contractility and calcium in single-cell cardiomyocytes.
dc.creator.none.fl_str_mv Marimon X
Esquinas F
Ferrer M
Cerrolaza M
Portela A
Benítez R
author Marimon X
author_facet Marimon X
Esquinas F
Ferrer M
Cerrolaza M
Portela A
Benítez R
author_role author
author2 Esquinas F
Ferrer M
Cerrolaza M
Portela A
Benítez R
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Calcium imaging
Cardiomyocyte
Cellular biomechanics
Continuum mechanics
Digital image correlation
Image processing
Strain analysis
topic Calcium imaging
Cardiomyocyte
Cellular biomechanics
Continuum mechanics
Digital image correlation
Image processing
Strain analysis
description The use of a video method based on the Digital Image Correlation (DIC) algorithm from experimental mechanics to estimate the displacements, strain field, and sarcolemma length in a beating single-cell cardiomyocyte is proposed in this work. The obtained deformation is then correlated with the calcium signal, from calcium imaging where fluorescent dyes sensitive to calcium Ca(2+) are used. Our proposed video-based method for simultaneous contraction and intracellular calcium analysis results in a low-cost, non-invasive, and label-free method. This technique has shown great advantages in long-term observations because this type of intervention-free measurement neutralizes the possible alteration in the beating cardiomyocyte introduced by other techniques for measuring cell contractility (e.g., Traction Force Microscopy, Atomic Force Microscopy, Microfabrication or Optical tweezers). Three tests were performed with synthetically augmented data from cardiomyocyte images to validate the robustness of the algorithm. First, a simulated rigid translation of a referenced image is applied, then a rotation, and finally a controlled longitudinal deformation of the referenced image, thus simulating a native realistic deformation. Finally, the proposed framework is evaluated with real experimental data. To validate contraction induced by intracellular calcium concentration, this signal is correlated with a new deformation measure proposed in this article, which is independent of cell orientation in the imaging setup. Finally, based on the displacements obtained by the DIC algorithm, the change in sarcolemma length in a contracting cardiomyocyte is calculated and its temporal correlation with the calcium signal is obtained.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=27209
url https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=27209
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv ELSEVIER
publisher.none.fl_str_mv ELSEVIER
dc.source.none.fl_str_mv Journal of the Mechanical Behavior of Biomedical Materials
ISSN: 17516161
ISSNe: 18780180
reponame:r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
instname:Fundació Sant Joan de Déu
instname_str Fundació Sant Joan de Déu
reponame_str r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
collection r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
repository.name.fl_str_mv
repository.mail.fl_str_mv
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