Enhanced denitrification in groundwater and sediments from a nitrate-contaminated aquifer after addition of pyrite
Using chemical, isotopic and microbiologic techniques we tested in laboratory experiments the extent to which the addition of pyrite to groundwater and sediments from a nitrate-contaminated aquifer could stimulate denitrification by indigenous bacteria. In addition to this biostimulated approach, a...
| Autores: | , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2011 |
| País: | España |
| Institución: | Universidad de Barcelona |
| Repositorio: | Dipòsit Digital de la UB |
| OAI Identifier: | oai:diposit.ub.edu:2445/155350 |
| Acceso en línea: | https://hdl.handle.net/2445/155350 |
| Access Level: | acceso abierto |
| Palabra clave: | Bioremediació Nitrats Desnitrificació Contaminació de l'aigua Hidrologia d'aigües subterrànies Bioremediation Nitrates Denitrification Water pollution Groundwater hydrology |
| id |
ES_d03fe2f482dc5c0168f64ab7a02e85fe |
|---|---|
| oai_identifier_str |
oai:diposit.ub.edu:2445/155350 |
| network_acronym_str |
ES |
| network_name_str |
España |
| repository_id_str |
|
| spelling |
Enhanced denitrification in groundwater and sediments from a nitrate-contaminated aquifer after addition of pyriteTorrentó, ClaraUrmeneta, JordiOtero Pérez, NeusSoler i Gil, AlbertViñas Canals, MarcCama i Robert, JordiBioremediacióNitratsDesnitrificacióContaminació de l'aiguaHidrologia d'aigües subterràniesBioremediationNitratesDenitrificationWater pollutionGroundwater hydrologyUsing chemical, isotopic and microbiologic techniques we tested in laboratory experiments the extent to which the addition of pyrite to groundwater and sediments from a nitrate-contaminated aquifer could stimulate denitrification by indigenous bacteria. In addition to this biostimulated approach, a combined biostimulated and bioaugmented treatment was also evaluated by inoculating the well-known autotrophic denitrifying bacterium Thiobacillus denitrificans. Results showed that the addition of pyrite enhanced nitrate removal and that denitrifying bacteria existing in the aquifer material were able to reduce nitrate using pyrite as the electron donor, obviating the need for the inoculation of T. denitrificans. The results of the 16S rRNA and nosZ gene-based DGGE and the quantitative PCR (qPCR) showed that the addition of pyrite led to an increase in the proportion of denitrifying bacteria and that bacterial populations closely related to the Xanthomonadaceae might probably be the autotrophic denitrifiers that used pyrite as the electron donor. Not only autotrophic but also heterotrophic denitrifying bacteria were stimulated through pyrite addition and both populations probably contributed to nitrate removal. Isotopic analyses (δ15N and δ18ONO3) were used to monitor enhanced denitrification and the N and O isotopic enrichment factors (−26.3±1.8¿ and −20.4± 1.3¿, respectively) allowed to calculate the degree of natural nitrate attenuation in the aquifer. Furthermore, flow-through experiments amended with pyrite confirmed the long-term efficiency of the process under the study conditions. Further research under field conditions is needed to determine whether stimulation of denitrification by pyrite addition constitutes a feasible bioremediation strategy for nitrate-contaminated aquifers.Elsevier B.V.2011info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/155350Articles publicats en revistes (Mineralogia, Petrologia i Geologia Aplicada)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésVersió postprint del document publicat a: https://doi.org/10.1016/j.chemgeo.2011.06.002Chemical Geology, 2011, vol. 287, num. 1-2, p. 90-101https://doi.org/10.1016/j.chemgeo.2011.06.002(c) Elsevier B.V., 2011info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1553502026-05-27T06:46:51Z |
| dc.title.none.fl_str_mv |
Enhanced denitrification in groundwater and sediments from a nitrate-contaminated aquifer after addition of pyrite |
| title |
Enhanced denitrification in groundwater and sediments from a nitrate-contaminated aquifer after addition of pyrite |
| spellingShingle |
Enhanced denitrification in groundwater and sediments from a nitrate-contaminated aquifer after addition of pyrite Torrentó, Clara Bioremediació Nitrats Desnitrificació Contaminació de l'aigua Hidrologia d'aigües subterrànies Bioremediation Nitrates Denitrification Water pollution Groundwater hydrology |
| title_short |
Enhanced denitrification in groundwater and sediments from a nitrate-contaminated aquifer after addition of pyrite |
| title_full |
Enhanced denitrification in groundwater and sediments from a nitrate-contaminated aquifer after addition of pyrite |
| title_fullStr |
Enhanced denitrification in groundwater and sediments from a nitrate-contaminated aquifer after addition of pyrite |
| title_full_unstemmed |
Enhanced denitrification in groundwater and sediments from a nitrate-contaminated aquifer after addition of pyrite |
| title_sort |
Enhanced denitrification in groundwater and sediments from a nitrate-contaminated aquifer after addition of pyrite |
| dc.creator.none.fl_str_mv |
Torrentó, Clara Urmeneta, Jordi Otero Pérez, Neus Soler i Gil, Albert Viñas Canals, Marc Cama i Robert, Jordi |
| author |
Torrentó, Clara |
| author_facet |
Torrentó, Clara Urmeneta, Jordi Otero Pérez, Neus Soler i Gil, Albert Viñas Canals, Marc Cama i Robert, Jordi |
| author_role |
author |
| author2 |
Urmeneta, Jordi Otero Pérez, Neus Soler i Gil, Albert Viñas Canals, Marc Cama i Robert, Jordi |
| author2_role |
author author author author author |
| dc.subject.none.fl_str_mv |
Bioremediació Nitrats Desnitrificació Contaminació de l'aigua Hidrologia d'aigües subterrànies Bioremediation Nitrates Denitrification Water pollution Groundwater hydrology |
| topic |
Bioremediació Nitrats Desnitrificació Contaminació de l'aigua Hidrologia d'aigües subterrànies Bioremediation Nitrates Denitrification Water pollution Groundwater hydrology |
| description |
Using chemical, isotopic and microbiologic techniques we tested in laboratory experiments the extent to which the addition of pyrite to groundwater and sediments from a nitrate-contaminated aquifer could stimulate denitrification by indigenous bacteria. In addition to this biostimulated approach, a combined biostimulated and bioaugmented treatment was also evaluated by inoculating the well-known autotrophic denitrifying bacterium Thiobacillus denitrificans. Results showed that the addition of pyrite enhanced nitrate removal and that denitrifying bacteria existing in the aquifer material were able to reduce nitrate using pyrite as the electron donor, obviating the need for the inoculation of T. denitrificans. The results of the 16S rRNA and nosZ gene-based DGGE and the quantitative PCR (qPCR) showed that the addition of pyrite led to an increase in the proportion of denitrifying bacteria and that bacterial populations closely related to the Xanthomonadaceae might probably be the autotrophic denitrifiers that used pyrite as the electron donor. Not only autotrophic but also heterotrophic denitrifying bacteria were stimulated through pyrite addition and both populations probably contributed to nitrate removal. Isotopic analyses (δ15N and δ18ONO3) were used to monitor enhanced denitrification and the N and O isotopic enrichment factors (−26.3±1.8¿ and −20.4± 1.3¿, respectively) allowed to calculate the degree of natural nitrate attenuation in the aquifer. Furthermore, flow-through experiments amended with pyrite confirmed the long-term efficiency of the process under the study conditions. Further research under field conditions is needed to determine whether stimulation of denitrification by pyrite addition constitutes a feasible bioremediation strategy for nitrate-contaminated aquifers. |
| publishDate |
2011 |
| dc.date.none.fl_str_mv |
2011 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion |
| format |
article |
| status_str |
acceptedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/2445/155350 |
| url |
https://hdl.handle.net/2445/155350 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Versió postprint del document publicat a: https://doi.org/10.1016/j.chemgeo.2011.06.002 Chemical Geology, 2011, vol. 287, num. 1-2, p. 90-101 https://doi.org/10.1016/j.chemgeo.2011.06.002 |
| dc.rights.none.fl_str_mv |
(c) Elsevier B.V., 2011 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
(c) Elsevier B.V., 2011 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier B.V. |
| publisher.none.fl_str_mv |
Elsevier B.V. |
| dc.source.none.fl_str_mv |
Articles publicats en revistes (Mineralogia, Petrologia i Geologia Aplicada) reponame:Dipòsit Digital de la UB instname:Universidad de Barcelona |
| instname_str |
Universidad de Barcelona |
| reponame_str |
Dipòsit Digital de la UB |
| collection |
Dipòsit Digital de la UB |
| repository.name.fl_str_mv |
|
| repository.mail.fl_str_mv |
|
| _version_ |
1869420157308239872 |
| score |
15,300724 |