Outer-inner membrane vesicles naturally secreted by gram-negative pathogenic bacteria.

Outer-inner membrane vesicles (O-IMVs) were recently described as a new type of membrane vesicle secreted by the Antarctic bacterium Shewanella vesiculosa M7T. Their formation is characterized by the protrusion of both outer and plasma membranes, which pulls cytoplasmic components into the vesicles....

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Detalles Bibliográficos
Autores: Pérez Cruz, Carla, Delgado, Lídia, López Iglesias, Carmen, Mercadé Gil, M. Elena
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/104307
Acceso en línea:https://hdl.handle.net/2445/104307
Access Level:acceso abierto
Palabra clave:Membranes cel·lulars
Bacteris gramnegatius
Bacteris patògens
Cell membranes
Gram-negative bacteria
Pathogenic bacteria
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spelling Outer-inner membrane vesicles naturally secreted by gram-negative pathogenic bacteria.Pérez Cruz, CarlaDelgado, LídiaLópez Iglesias, CarmenMercadé Gil, M. ElenaMembranes cel·lularsBacteris gramnegatiusBacteris patògensCell membranesGram-negative bacteriaPathogenic bacteriaOuter-inner membrane vesicles (O-IMVs) were recently described as a new type of membrane vesicle secreted by the Antarctic bacterium Shewanella vesiculosa M7T. Their formation is characterized by the protrusion of both outer and plasma membranes, which pulls cytoplasmic components into the vesicles. To demonstrate that this is not a singular phenomenon in a bacterium occurring in an extreme environment, the identification of O-IMVs in pathogenic bacteria was undertaken. With this aim, a structural study by Transmission Electron Microscopy (TEM) and Cryo-transmission electron microscopy (Cryo-TEM) was carried out, confirming that O-IMVs are also secreted by Gram-negative pathogenic bacteria such as Neisseria gonorrhoeae, Pseudomonas aeruginosa PAO1 and Acinetobacter baumannii AB41, in which they represent between 0.23% and 1.2% of total vesicles produced. DNA and ATP, which are components solely found in the cell cytoplasm, were identified within membrane vesicles of these strains. The presence of DNA inside the O-IMVs produced by N. gonorrhoeae was confirmed by gold DNA immunolabeling with a specific monoclonal IgM against double-stranded DNA. A proteomic analysis of N. gonorrhoeae-derived membrane vesicles identified proteins from the cytoplasm and plasma membrane. This confirmation of O-IMV extends the hitherto uniform definition of membrane vesicles in Gram-negative bacteria and explains the presence of components in membrane vesicles such as DNA, cytoplasmic and inner membrane proteins, as well as ATP, detected for the first time. The production of these O-IMVs by pathogenic Gram-negative bacteria opens up new areas of study related to their involvement in lateral gene transfer, the transfer of cytoplasmic proteins, as well as the functionality and role of ATP detected in these new vesiclesPublic Library of Science (PLoS)2016201620152016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion18 p.application/pdfhttps://hdl.handle.net/2445/104307Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1371/journal.pone.0116896PLoS One, 2015, vol. 10, num. 1, p. e0116896https://doi.org/10.1371/journal.pone.0116896cc-by (c) Pérez-Cruz, Carla et al., 2015http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1043072026-05-29T05:05:01Z
dc.title.none.fl_str_mv Outer-inner membrane vesicles naturally secreted by gram-negative pathogenic bacteria.
title Outer-inner membrane vesicles naturally secreted by gram-negative pathogenic bacteria.
spellingShingle Outer-inner membrane vesicles naturally secreted by gram-negative pathogenic bacteria.
Pérez Cruz, Carla
Membranes cel·lulars
Bacteris gramnegatius
Bacteris patògens
Cell membranes
Gram-negative bacteria
Pathogenic bacteria
title_short Outer-inner membrane vesicles naturally secreted by gram-negative pathogenic bacteria.
title_full Outer-inner membrane vesicles naturally secreted by gram-negative pathogenic bacteria.
title_fullStr Outer-inner membrane vesicles naturally secreted by gram-negative pathogenic bacteria.
title_full_unstemmed Outer-inner membrane vesicles naturally secreted by gram-negative pathogenic bacteria.
title_sort Outer-inner membrane vesicles naturally secreted by gram-negative pathogenic bacteria.
dc.creator.none.fl_str_mv Pérez Cruz, Carla
Delgado, Lídia
López Iglesias, Carmen
Mercadé Gil, M. Elena
author Pérez Cruz, Carla
author_facet Pérez Cruz, Carla
Delgado, Lídia
López Iglesias, Carmen
Mercadé Gil, M. Elena
author_role author
author2 Delgado, Lídia
López Iglesias, Carmen
Mercadé Gil, M. Elena
author2_role author
author
author
dc.subject.none.fl_str_mv Membranes cel·lulars
Bacteris gramnegatius
Bacteris patògens
Cell membranes
Gram-negative bacteria
Pathogenic bacteria
topic Membranes cel·lulars
Bacteris gramnegatius
Bacteris patògens
Cell membranes
Gram-negative bacteria
Pathogenic bacteria
description Outer-inner membrane vesicles (O-IMVs) were recently described as a new type of membrane vesicle secreted by the Antarctic bacterium Shewanella vesiculosa M7T. Their formation is characterized by the protrusion of both outer and plasma membranes, which pulls cytoplasmic components into the vesicles. To demonstrate that this is not a singular phenomenon in a bacterium occurring in an extreme environment, the identification of O-IMVs in pathogenic bacteria was undertaken. With this aim, a structural study by Transmission Electron Microscopy (TEM) and Cryo-transmission electron microscopy (Cryo-TEM) was carried out, confirming that O-IMVs are also secreted by Gram-negative pathogenic bacteria such as Neisseria gonorrhoeae, Pseudomonas aeruginosa PAO1 and Acinetobacter baumannii AB41, in which they represent between 0.23% and 1.2% of total vesicles produced. DNA and ATP, which are components solely found in the cell cytoplasm, were identified within membrane vesicles of these strains. The presence of DNA inside the O-IMVs produced by N. gonorrhoeae was confirmed by gold DNA immunolabeling with a specific monoclonal IgM against double-stranded DNA. A proteomic analysis of N. gonorrhoeae-derived membrane vesicles identified proteins from the cytoplasm and plasma membrane. This confirmation of O-IMV extends the hitherto uniform definition of membrane vesicles in Gram-negative bacteria and explains the presence of components in membrane vesicles such as DNA, cytoplasmic and inner membrane proteins, as well as ATP, detected for the first time. The production of these O-IMVs by pathogenic Gram-negative bacteria opens up new areas of study related to their involvement in lateral gene transfer, the transfer of cytoplasmic proteins, as well as the functionality and role of ATP detected in these new vesicles
publishDate 2015
dc.date.none.fl_str_mv 2015
2016
2016
2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/104307
url https://hdl.handle.net/2445/104307
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0116896
PLoS One, 2015, vol. 10, num. 1, p. e0116896
https://doi.org/10.1371/journal.pone.0116896
dc.rights.none.fl_str_mv cc-by (c) Pérez-Cruz, Carla et al., 2015
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Pérez-Cruz, Carla et al., 2015
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 18 p.
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science (PLoS)
publisher.none.fl_str_mv Public Library of Science (PLoS)
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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