First description of two moderately halophilic and psychrotolerant Mycoplasma species isolated from cephalopods and proposal of Mycoplasma marinum sp. nov. and Mycoplasma todarodis sp. nov

Two moderately halophilic and psychrotolerant new Mycoplasma species were isolated from common cephalopods. Three strains were isolated in pure culture from two individual European flying squid (Todarodes sagittatus), and two individual octopuses (Octopus vulgaris). The strains showed optimal growth...

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Bibliographic Details
Authors: Ramírez, Ana Sofía, Vega-Orellana, Orestes, Viver, Tomeu, Poveda, José B., Rosales, Rubén S., Poveda, Carlos G., Spergser, Joachim, Szostak, Michael P., Caballero, María José, Ressel, Lorenzo, Bradbury, Janet M., Tavío, M. Mar, Karthikeyan, Smruthi, Amann, Rudolf, Konstantinidis, Konstantinos T., Rosselló-Mora, Ramón
Format: article
Status:Versión aceptada para publicación
Publication Date:2019
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:dnet:digitalcsic_::aa6aea185309d154181b768029632fbf
Online Access:http://hdl.handle.net/10261/204621
Access Level:Open access
Keyword:Mollicutes
Mycoplasma sp. nov.
Octopus vulgaris
Todarodes sagittatus
Moderately halophilic bacteria
Psychrotolerant
Description
Summary:Two moderately halophilic and psychrotolerant new Mycoplasma species were isolated from common cephalopods. Three strains were isolated in pure culture from two individual European flying squid (Todarodes sagittatus), and two individual octopuses (Octopus vulgaris). The strains showed optimal growth at 25 °C and a salinity of 3% (w/v) NaCl. Molecular analyses revealed that the isolates belonged to two new, but phylogenetically related species, divergent from all previously described Mollicutes, representing the first marine isolates of the class, and also the first Mycoplasma strains for which NaCl requirement has been demonstrated. A genome search against all available marine metagenomes and 16S rRNA gene databases indicated that these two species represent a novel non-free-living marine lineage of Mollicutes, specifically associated with marine animals. Morphology and physiology were compatible with other members of this group, and genomic and phenotypic analyses demonstrated that these organisms represent two novel species of the genus Mycoplasma, for which the names Mycoplasma marinum sp. nov. and Mycoplasma todarodis sp. nov. are proposed; the type strains are PET (DSM 105487T, CIP 111404T) and 5HT (DSM 105,488T, CIP 111405T), respectively.

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