Interfacial activity of phasin PhaF from Pseudomonas putida KT2440 at hydrophobic-hydrophilic biointerfaces

37 p.-8 fig.-2 tab.

Detalhes bibliográficos
Autores: Mato, Aranzazu, Tarazona, Natalia A., Hidalgo, Alberto, Cruz, Antonio, Jiménez, Mercedes, Pérez-Gil, Jesús, Prieto, María Auxiliadora
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2019
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/175266
Acesso em linha:http://hdl.handle.net/10261/175266
Access Level:acceso abierto
Palavra-chave:Phasins
Polyhydroxyalkanoates
Biosurfactants
Pseudomonas putida
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spelling Interfacial activity of phasin PhaF from Pseudomonas putida KT2440 at hydrophobic-hydrophilic biointerfacesMato, AranzazuTarazona, Natalia A.Hidalgo, AlbertoCruz, AntonioJiménez, MercedesPérez-Gil, JesúsPrieto, María AuxiliadoraPhasinsPolyhydroxyalkanoatesBiosurfactantsPseudomonas putida37 p.-8 fig.-2 tab.Phasins, the major proteins coating polyhydroxyalkanoate (PHA) granules, have been proposed as suitable biosurfactants for multiple applications because of their amphiphilic nature. In this work, we analyzed the interfacial activity of the amphiphilic α-helical phasin PhaF from Pseudomonas putida KT2440 at different hydrophobic–hydrophilic interfacial environments. The binding of PhaF to surfaces containing PHA or phospholipids, postulated as structural components of PHA granules, was confirmed in vitro using supported lipid bilayers and confocal microscopy, with polyhydroxyoctanoate-co-hexanoate P(HO-co-HHx) and Escherichia coli lipid extract as model systems. The surfactant-like capabilities of PhaF were determined by measuring changes in surface pressure in Langmuir devices. PhaF spontaneously adsorbed at the air–water interface, reducing the surface tension from 72 mN/m (water surface tension at 25 °C) to 50 mN/m. The differences in the adsorption of the protein in the presence of different phospholipid films showed a marked preference for phosphatidylglycerol species, such as 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol. The PHA-binding domain of PhaF (BioF) conserved a similar surface activity to PhaF, suggesting that it is responsible for the surfactant properties of the whole protein. These new findings not only increase our knowledge about the role of phasins in the PHA machinery but also open new outlooks for the application of these proteins as biosurfactants.This work was supported by the European Union’s Horizon 2020 Research and innovation programme, grant agreement no. 633962 (P4SB), the Community of Madrid (P2013/MIT2807) and the Spanish Ministry of Science, Innovation and Universities (BIO2017-83448-R, BIO2015-67930-R).Peer reviewedAmerican Chemical SocietyEuropean CommissionComunidad de MadridMinisterio de Ciencia, Innovación y Universidades (España)Cruz, Antonio [0000-0002-0465-4111]Jiménez, Mercedes [0000-0003-2006-1903]Pérez-Gil, Jesús [0000-0003-3587-7147]Prieto, María Auxiliadora [0000-0002-8038-1223]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/175266reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/H2020/633962MICIU/ICTI2017-2020/BIO2017-83448-Rinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2015-67930-Rhttps://doi.org/10.1021/acs.langmuir.8b03036Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1752662026-05-22T06:33:51Z
dc.title.none.fl_str_mv Interfacial activity of phasin PhaF from Pseudomonas putida KT2440 at hydrophobic-hydrophilic biointerfaces
title Interfacial activity of phasin PhaF from Pseudomonas putida KT2440 at hydrophobic-hydrophilic biointerfaces
spellingShingle Interfacial activity of phasin PhaF from Pseudomonas putida KT2440 at hydrophobic-hydrophilic biointerfaces
Mato, Aranzazu
Phasins
Polyhydroxyalkanoates
Biosurfactants
Pseudomonas putida
title_short Interfacial activity of phasin PhaF from Pseudomonas putida KT2440 at hydrophobic-hydrophilic biointerfaces
title_full Interfacial activity of phasin PhaF from Pseudomonas putida KT2440 at hydrophobic-hydrophilic biointerfaces
title_fullStr Interfacial activity of phasin PhaF from Pseudomonas putida KT2440 at hydrophobic-hydrophilic biointerfaces
title_full_unstemmed Interfacial activity of phasin PhaF from Pseudomonas putida KT2440 at hydrophobic-hydrophilic biointerfaces
title_sort Interfacial activity of phasin PhaF from Pseudomonas putida KT2440 at hydrophobic-hydrophilic biointerfaces
dc.creator.none.fl_str_mv Mato, Aranzazu
Tarazona, Natalia A.
Hidalgo, Alberto
Cruz, Antonio
Jiménez, Mercedes
Pérez-Gil, Jesús
Prieto, María Auxiliadora
author Mato, Aranzazu
author_facet Mato, Aranzazu
Tarazona, Natalia A.
Hidalgo, Alberto
Cruz, Antonio
Jiménez, Mercedes
Pérez-Gil, Jesús
Prieto, María Auxiliadora
author_role author
author2 Tarazona, Natalia A.
Hidalgo, Alberto
Cruz, Antonio
Jiménez, Mercedes
Pérez-Gil, Jesús
Prieto, María Auxiliadora
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Comunidad de Madrid
Ministerio de Ciencia, Innovación y Universidades (España)
Cruz, Antonio [0000-0002-0465-4111]
Jiménez, Mercedes [0000-0003-2006-1903]
Pérez-Gil, Jesús [0000-0003-3587-7147]
Prieto, María Auxiliadora [0000-0002-8038-1223]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Phasins
Polyhydroxyalkanoates
Biosurfactants
Pseudomonas putida
topic Phasins
Polyhydroxyalkanoates
Biosurfactants
Pseudomonas putida
description 37 p.-8 fig.-2 tab.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/175266
url http://hdl.handle.net/10261/175266
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/H2020/633962
MICIU/ICTI2017-2020/BIO2017-83448-R
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2015-67930-R
https://doi.org/10.1021/acs.langmuir.8b03036
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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