Chitosan-based sustained expression of sterol regulatory element-binding protein 1a stimulates hepatic glucose oxidation and growth in <em>Sparus aurata</em>

The administration of a single dose of chitosan nanoparticles driving the expression of sterol regulatory element-binding protein 1a (SREBP1a) was recently associated with the enhanced conversion of carbohydrates into lipids. To address the effects of the long-lasting expression of SREBP1a on the gr...

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Autores: Rashidpour, Ania, Wu, Yuanbing, Almajano Pablos, Ma. Pilar (María Pilar), Fàbregas, Anna, Metón Teijeiro, Isidoro
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/218827
Acceso en línea:https://hdl.handle.net/2445/218827
Access Level:acceso abierto
Palabra clave:Orada
Quitosan
Fetge
Creixement
Sparus aurata
Chitosan
Liver
Growth
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spelling Chitosan-based sustained expression of sterol regulatory element-binding protein 1a stimulates hepatic glucose oxidation and growth in <em>Sparus aurata</em>Rashidpour, AniaWu, YuanbingAlmajano Pablos, Ma. Pilar (María Pilar)Fàbregas, AnnaMetón Teijeiro, IsidoroOradaQuitosanFetgeCreixementSparus aurataChitosanLiverGrowthThe administration of a single dose of chitosan nanoparticles driving the expression of sterol regulatory element-binding protein 1a (SREBP1a) was recently associated with the enhanced conversion of carbohydrates into lipids. To address the effects of the long-lasting expression of SREBP1a on the growth and liver intermediary metabolism of carnivorous fish, chitosan-tripolyphosphate (TPP) nanoparticles complexed with a plasmid expressing the N terminal active domain of hamster SREBP1a (pSG5-SREBP1a) were injected intraperitoneally every 4 weeks (three doses in total) to gilthead sea bream (Sparus aurata) fed high-protein-low-carbohydrate and low-protein-high-carbohydrate diets. Following 70 days of treatment, chitosan-TPP-pSG5-SREBP1a nanoparticles led to the sustained upregulation of SREBP1a in the liver of S. aurata. Independently of the diet, SREBP1a overexpression significantly increased their weight gain, specific growth rate, and protein efficiency ratio but decreased their feed conversion ratio. In agreement with an improved conversion of dietary carbohydrates into lipids, SREBP1a expression increased serum triglycerides and cholesterol as well as hepatic glucose oxidation via glycolysis and the pentose phosphate pathway, while not affecting gluconeogenesis and transamination. Our findings support that the periodical administration of chitosan-TPP-DNA nanoparticles to overexpress SREBP1a in the liver enhanced the growth performance of S. aurata through a mechanism that enabled protein sparing by enhancing dietary carbohydrate metabolisation.MDPI2025202520232025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion16 p.application/pdfhttps://hdl.handle.net/2445/218827Articles publicats en revistes (Bioquímica i Fisiologia)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.3390/md21110562Marine Drugs, 2023, vol. 21, num.11https://doi.org/10.3390/md21110562cc-by (c) Rashidpour, A. et al., 2023http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/2188272026-05-29T05:05:01Z
dc.title.none.fl_str_mv Chitosan-based sustained expression of sterol regulatory element-binding protein 1a stimulates hepatic glucose oxidation and growth in <em>Sparus aurata</em>
title Chitosan-based sustained expression of sterol regulatory element-binding protein 1a stimulates hepatic glucose oxidation and growth in <em>Sparus aurata</em>
spellingShingle Chitosan-based sustained expression of sterol regulatory element-binding protein 1a stimulates hepatic glucose oxidation and growth in <em>Sparus aurata</em>
Rashidpour, Ania
Orada
Quitosan
Fetge
Creixement
Sparus aurata
Chitosan
Liver
Growth
title_short Chitosan-based sustained expression of sterol regulatory element-binding protein 1a stimulates hepatic glucose oxidation and growth in <em>Sparus aurata</em>
title_full Chitosan-based sustained expression of sterol regulatory element-binding protein 1a stimulates hepatic glucose oxidation and growth in <em>Sparus aurata</em>
title_fullStr Chitosan-based sustained expression of sterol regulatory element-binding protein 1a stimulates hepatic glucose oxidation and growth in <em>Sparus aurata</em>
title_full_unstemmed Chitosan-based sustained expression of sterol regulatory element-binding protein 1a stimulates hepatic glucose oxidation and growth in <em>Sparus aurata</em>
title_sort Chitosan-based sustained expression of sterol regulatory element-binding protein 1a stimulates hepatic glucose oxidation and growth in <em>Sparus aurata</em>
dc.creator.none.fl_str_mv Rashidpour, Ania
Wu, Yuanbing
Almajano Pablos, Ma. Pilar (María Pilar)
Fàbregas, Anna
Metón Teijeiro, Isidoro
author Rashidpour, Ania
author_facet Rashidpour, Ania
Wu, Yuanbing
Almajano Pablos, Ma. Pilar (María Pilar)
Fàbregas, Anna
Metón Teijeiro, Isidoro
author_role author
author2 Wu, Yuanbing
Almajano Pablos, Ma. Pilar (María Pilar)
Fàbregas, Anna
Metón Teijeiro, Isidoro
author2_role author
author
author
author
dc.subject.none.fl_str_mv Orada
Quitosan
Fetge
Creixement
Sparus aurata
Chitosan
Liver
Growth
topic Orada
Quitosan
Fetge
Creixement
Sparus aurata
Chitosan
Liver
Growth
description The administration of a single dose of chitosan nanoparticles driving the expression of sterol regulatory element-binding protein 1a (SREBP1a) was recently associated with the enhanced conversion of carbohydrates into lipids. To address the effects of the long-lasting expression of SREBP1a on the growth and liver intermediary metabolism of carnivorous fish, chitosan-tripolyphosphate (TPP) nanoparticles complexed with a plasmid expressing the N terminal active domain of hamster SREBP1a (pSG5-SREBP1a) were injected intraperitoneally every 4 weeks (three doses in total) to gilthead sea bream (Sparus aurata) fed high-protein-low-carbohydrate and low-protein-high-carbohydrate diets. Following 70 days of treatment, chitosan-TPP-pSG5-SREBP1a nanoparticles led to the sustained upregulation of SREBP1a in the liver of S. aurata. Independently of the diet, SREBP1a overexpression significantly increased their weight gain, specific growth rate, and protein efficiency ratio but decreased their feed conversion ratio. In agreement with an improved conversion of dietary carbohydrates into lipids, SREBP1a expression increased serum triglycerides and cholesterol as well as hepatic glucose oxidation via glycolysis and the pentose phosphate pathway, while not affecting gluconeogenesis and transamination. Our findings support that the periodical administration of chitosan-TPP-DNA nanoparticles to overexpress SREBP1a in the liver enhanced the growth performance of S. aurata through a mechanism that enabled protein sparing by enhancing dietary carbohydrate metabolisation.
publishDate 2023
dc.date.none.fl_str_mv 2023
2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/218827
url https://hdl.handle.net/2445/218827
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/md21110562
Marine Drugs, 2023, vol. 21, num.11
https://doi.org/10.3390/md21110562
dc.rights.none.fl_str_mv cc-by (c) Rashidpour, A. et al., 2023
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Rashidpour, A. et al., 2023
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 16 p.
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Fisiologia)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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