Changes of bone turnover markers in long bone nonunions treated with a regenerative approach

In this clinical trial, we investigated if biochemical bone turnover markers (BTM) changed according to the progression of bone healing induced by autologous expanded MSC combined with a biphasic calcium phosphate in patients with delayed union or nonunion of long bone fractures. Bone formation mark...

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Detalhes bibliográficos
Autores: Granchi, Donatella, Gómez Barrena, Enrique, Rojewski, Markus, Rosset, Philippe, Layrolle, Pierre, Spazzoli, Benedetta, Donati, Davide Maria, Ciapetti, Gabriela
Formato: artículo
Fecha de publicación:2017
País:España
Recursos:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/680538
Acesso em linha:http://hdl.handle.net/10486/680538
https://dx.doi.org/10.1155/2017/3674045
Access Level:acceso abierto
Palavra-chave:Bone turnover markers (BTM)
Biphasic calcium phosphate
Regenerative treatment
Changes
Bone nonunions
Medicina
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spelling Changes of bone turnover markers in long bone nonunions treated with a regenerative approachGranchi, DonatellaGómez Barrena, EnriqueRojewski, MarkusRosset, PhilippeLayrolle, PierreSpazzoli, BenedettaDonati, Davide MariaCiapetti, GabrielaBone turnover markers (BTM)Biphasic calcium phosphateRegenerative treatmentChangesBone nonunionsMedicinaIn this clinical trial, we investigated if biochemical bone turnover markers (BTM) changed according to the progression of bone healing induced by autologous expanded MSC combined with a biphasic calcium phosphate in patients with delayed union or nonunion of long bone fractures. Bone formation markers, bone resorption markers, and osteoclast regulatory proteins were measured by enzymatic immunoassay before surgery and after 6, 12, and 24 weeks. A satisfactory bone healing was obtained in 23 out of 24 patients. Nine subjects reached a good consolidation already at 12 weeks, and they were considered as the "early consolidation" group. We found that bone-specific alkaline phosphatase (BAP), C-terminal propeptide of type I procollagen (PICP), and beta crosslaps collagen (CTX) changed after the regenerative treatment, BAP and CTX correlated to the imaging results collected at 12 and 24 weeks, and BAP variation along the healing course differed in patients who had an "early consolidation." A remarkable decrease in BAP and PICP was observed at all time points in a single patient who experienced a treatment failure, but the predictive value of BTM changes cannot be determined. Our findings suggest that BTM are promising tools for monitoring cell therapy efficacy in bone nonunions, but studies with larger patient numbers are required to confirm these preliminary resultsThis work was supported by the EC, Seventh Framework Program (FP7), through the REBORNE Project, Grant Agreement no. 241879 and partially by the Italian Ministry of Health, Ricerca Corrente, project “Strategie di rigenerazione ossea: dall’interazione materiale/cellule dell’osso ai markers riparativi” (2013–2016)HindawiDepartamento de CirugíaFacultad de MedicinaInstituto de Investigación Sanitaria del Hospital Universitario de La Paz (IdiPAZ)20172017-06-20research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/680538https://dx.doi.org/10.1155/2017/3674045reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/6805382026-06-23T12:46:27Z
dc.title.none.fl_str_mv Changes of bone turnover markers in long bone nonunions treated with a regenerative approach
title Changes of bone turnover markers in long bone nonunions treated with a regenerative approach
spellingShingle Changes of bone turnover markers in long bone nonunions treated with a regenerative approach
Granchi, Donatella
Bone turnover markers (BTM)
Biphasic calcium phosphate
Regenerative treatment
Changes
Bone nonunions
Medicina
title_short Changes of bone turnover markers in long bone nonunions treated with a regenerative approach
title_full Changes of bone turnover markers in long bone nonunions treated with a regenerative approach
title_fullStr Changes of bone turnover markers in long bone nonunions treated with a regenerative approach
title_full_unstemmed Changes of bone turnover markers in long bone nonunions treated with a regenerative approach
title_sort Changes of bone turnover markers in long bone nonunions treated with a regenerative approach
dc.creator.none.fl_str_mv Granchi, Donatella
Gómez Barrena, Enrique
Rojewski, Markus
Rosset, Philippe
Layrolle, Pierre
Spazzoli, Benedetta
Donati, Davide Maria
Ciapetti, Gabriela
author Granchi, Donatella
author_facet Granchi, Donatella
Gómez Barrena, Enrique
Rojewski, Markus
Rosset, Philippe
Layrolle, Pierre
Spazzoli, Benedetta
Donati, Davide Maria
Ciapetti, Gabriela
author_role author
author2 Gómez Barrena, Enrique
Rojewski, Markus
Rosset, Philippe
Layrolle, Pierre
Spazzoli, Benedetta
Donati, Davide Maria
Ciapetti, Gabriela
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Cirugía
Facultad de Medicina
Instituto de Investigación Sanitaria del Hospital Universitario de La Paz (IdiPAZ)
dc.subject.none.fl_str_mv Bone turnover markers (BTM)
Biphasic calcium phosphate
Regenerative treatment
Changes
Bone nonunions
Medicina
topic Bone turnover markers (BTM)
Biphasic calcium phosphate
Regenerative treatment
Changes
Bone nonunions
Medicina
description In this clinical trial, we investigated if biochemical bone turnover markers (BTM) changed according to the progression of bone healing induced by autologous expanded MSC combined with a biphasic calcium phosphate in patients with delayed union or nonunion of long bone fractures. Bone formation markers, bone resorption markers, and osteoclast regulatory proteins were measured by enzymatic immunoassay before surgery and after 6, 12, and 24 weeks. A satisfactory bone healing was obtained in 23 out of 24 patients. Nine subjects reached a good consolidation already at 12 weeks, and they were considered as the "early consolidation" group. We found that bone-specific alkaline phosphatase (BAP), C-terminal propeptide of type I procollagen (PICP), and beta crosslaps collagen (CTX) changed after the regenerative treatment, BAP and CTX correlated to the imaging results collected at 12 and 24 weeks, and BAP variation along the healing course differed in patients who had an "early consolidation." A remarkable decrease in BAP and PICP was observed at all time points in a single patient who experienced a treatment failure, but the predictive value of BTM changes cannot be determined. Our findings suggest that BTM are promising tools for monitoring cell therapy efficacy in bone nonunions, but studies with larger patient numbers are required to confirm these preliminary results
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-06-20
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/680538
https://dx.doi.org/10.1155/2017/3674045
url http://hdl.handle.net/10486/680538
https://dx.doi.org/10.1155/2017/3674045
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hindawi
publisher.none.fl_str_mv Hindawi
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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