Genome reduction and potential metabolic complementation of the dual endosymbionts in the whitefly <i>Bemisia tabaci</i>

Background: The whitefly Bemisia tabaci is an important agricultural pest with global distribution. This phloem-sap feeder harbors a primary symbiont, "Candidatus Portiera aleyrodidarum", which compensates for the deficient nutritional composition of its food sources, and a variety of seco...

Descripción completa

Detalles Bibliográficos
Autores: Rao Q, Rollat-Farnier PA, Zhu DT, Santos-Garcia D, Silva FJ, Moya A, Latorre A, Klein CC, Vavre F, Sagot MF, Liu SS, Mouton L, Wang XW
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Institución:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
Repositorio:r-FISABIO. Repositorio Institucional de Producción Científica
OAI Identifier:oai:fisabio.fundanetsuite.com:p4664
Acceso en línea:https://fisabio.portalinvestigacion.com/publicaciones/4664
Access Level:acceso abierto
Palabra clave:Endosymbiont
Genome
Hamiltonella
Portiera
Whitefly
Metabolic complementation
id ES_cfa5a9d9508a96eac3bbfc73166a1094
oai_identifier_str oai:fisabio.fundanetsuite.com:p4664
network_acronym_str ES
network_name_str España
repository_id_str
spelling Genome reduction and potential metabolic complementation of the dual endosymbionts in the whitefly <i>Bemisia tabaci</i>Rao QRollat-Farnier PAZhu DTSantos-Garcia DSilva FJMoya ALatorre AKlein CCVavre FSagot MFLiu SSMouton LWang XWEndosymbiontGenomeHamiltonellaPortieraWhiteflyMetabolic complementationBackground: The whitefly Bemisia tabaci is an important agricultural pest with global distribution. This phloem-sap feeder harbors a primary symbiont, "Candidatus Portiera aleyrodidarum", which compensates for the deficient nutritional composition of its food sources, and a variety of secondary symbionts. Interestingly, all of these secondary symbionts are found in co-localization with the primary symbiont within the same bacteriocytes, which should favor the evolution of strong interactions between symbionts. Results: In this paper, we analyzed the genome sequences of the primary symbiont Portiera and of the secondary symbiont Hamiltonella in the B. tabaci Mediterranean (MED) species in order to gain insight into the metabolic role of each symbiont in the biology of their host. The genome sequences of the uncultured symbionts Portiera and Hamiltonella were obtained from one single bacteriocyte of MED B. tabaci. As already reported, the genome of Portiera is highly reduced (357 kb), but has kept a number of genes encoding most essential amino-acids and carotenoids. On the other hand, Portiera lacks almost all the genes involved in the synthesis of vitamins and cofactors. Moreover, some pathways are incomplete, notably those involved in the synthesis of some essential amino-acids. Interestingly, the genome of Hamiltonella revealed that this secondary symbiont can not only provide vitamins and cofactors, but also complete the missing steps of some of the pathways of Portiera. In addition, some critical amino-acid biosynthetic genes are missing in the two symbiotic genomes, but analysis of whitefly transcriptome suggests that the missing steps may be performed by the whitefly itself or its microbiota. Conclusions: These data suggest that Portiera and Hamiltonella are not only complementary but could also be mutually dependent to provide a full complement of nutrients to their host. Altogether, these results illustrate how functional redundancies can lead to gene losses in the genomes of the different symbiotic partners, reinforcing their inter-dependency.BMC2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://fisabio.portalinvestigacion.com/publicaciones/4664BMC GENOMICSISSN: 14712164reponame:r-FISABIO. Repositorio Institucional de Producción Científicainstname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)Inglésinfo:eu-repo/semantics/openAccessoai:fisabio.fundanetsuite.com:p46642026-06-11T12:45:17Z
dc.title.none.fl_str_mv Genome reduction and potential metabolic complementation of the dual endosymbionts in the whitefly <i>Bemisia tabaci</i>
title Genome reduction and potential metabolic complementation of the dual endosymbionts in the whitefly <i>Bemisia tabaci</i>
spellingShingle Genome reduction and potential metabolic complementation of the dual endosymbionts in the whitefly <i>Bemisia tabaci</i>
Rao Q
Endosymbiont
Genome
Hamiltonella
Portiera
Whitefly
Metabolic complementation
title_short Genome reduction and potential metabolic complementation of the dual endosymbionts in the whitefly <i>Bemisia tabaci</i>
title_full Genome reduction and potential metabolic complementation of the dual endosymbionts in the whitefly <i>Bemisia tabaci</i>
title_fullStr Genome reduction and potential metabolic complementation of the dual endosymbionts in the whitefly <i>Bemisia tabaci</i>
title_full_unstemmed Genome reduction and potential metabolic complementation of the dual endosymbionts in the whitefly <i>Bemisia tabaci</i>
title_sort Genome reduction and potential metabolic complementation of the dual endosymbionts in the whitefly <i>Bemisia tabaci</i>
dc.creator.none.fl_str_mv Rao Q
Rollat-Farnier PA
Zhu DT
Santos-Garcia D
Silva FJ
Moya A
Latorre A
Klein CC
Vavre F
Sagot MF
Liu SS
Mouton L
Wang XW
author Rao Q
author_facet Rao Q
Rollat-Farnier PA
Zhu DT
Santos-Garcia D
Silva FJ
Moya A
Latorre A
Klein CC
Vavre F
Sagot MF
Liu SS
Mouton L
Wang XW
author_role author
author2 Rollat-Farnier PA
Zhu DT
Santos-Garcia D
Silva FJ
Moya A
Latorre A
Klein CC
Vavre F
Sagot MF
Liu SS
Mouton L
Wang XW
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Endosymbiont
Genome
Hamiltonella
Portiera
Whitefly
Metabolic complementation
topic Endosymbiont
Genome
Hamiltonella
Portiera
Whitefly
Metabolic complementation
description Background: The whitefly Bemisia tabaci is an important agricultural pest with global distribution. This phloem-sap feeder harbors a primary symbiont, "Candidatus Portiera aleyrodidarum", which compensates for the deficient nutritional composition of its food sources, and a variety of secondary symbionts. Interestingly, all of these secondary symbionts are found in co-localization with the primary symbiont within the same bacteriocytes, which should favor the evolution of strong interactions between symbionts. Results: In this paper, we analyzed the genome sequences of the primary symbiont Portiera and of the secondary symbiont Hamiltonella in the B. tabaci Mediterranean (MED) species in order to gain insight into the metabolic role of each symbiont in the biology of their host. The genome sequences of the uncultured symbionts Portiera and Hamiltonella were obtained from one single bacteriocyte of MED B. tabaci. As already reported, the genome of Portiera is highly reduced (357 kb), but has kept a number of genes encoding most essential amino-acids and carotenoids. On the other hand, Portiera lacks almost all the genes involved in the synthesis of vitamins and cofactors. Moreover, some pathways are incomplete, notably those involved in the synthesis of some essential amino-acids. Interestingly, the genome of Hamiltonella revealed that this secondary symbiont can not only provide vitamins and cofactors, but also complete the missing steps of some of the pathways of Portiera. In addition, some critical amino-acid biosynthetic genes are missing in the two symbiotic genomes, but analysis of whitefly transcriptome suggests that the missing steps may be performed by the whitefly itself or its microbiota. Conclusions: These data suggest that Portiera and Hamiltonella are not only complementary but could also be mutually dependent to provide a full complement of nutrients to their host. Altogether, these results illustrate how functional redundancies can lead to gene losses in the genomes of the different symbiotic partners, reinforcing their inter-dependency.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://fisabio.portalinvestigacion.com/publicaciones/4664
url https://fisabio.portalinvestigacion.com/publicaciones/4664
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv BMC GENOMICS
ISSN: 14712164
reponame:r-FISABIO. Repositorio Institucional de Producción Científica
instname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
instname_str Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
reponame_str r-FISABIO. Repositorio Institucional de Producción Científica
collection r-FISABIO. Repositorio Institucional de Producción Científica
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869420101786140672
score 15,811543

Similares en LA Referencia