Bioinspired three-dimensional multifunctional systems to enhance the therapeutic potential of mesenchymal stromal cells

419 p.

Detalles Bibliográficos
Autor: González Pujana, Ainhoa
Tipo de recurso: tesis doctoral
Fecha de publicación:2019
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/42317
Acceso en línea:http://hdl.handle.net/10810/42317
Access Level:acceso abierto
Palabra clave:cultivo celular
evaluación de fármacos
preparación de fármacos
cell culture
evaluation of drugs
preparation of drugs
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spelling Bioinspired three-dimensional multifunctional systems to enhance the therapeutic potential of mesenchymal stromal cellsZelula estromal mesenkimalen eragin immunomodulatzailea sustatzeko bioinspiraturiko hiru dimentsiotako sistema funtzio anizdunakGonzález Pujana, Ainhoacultivo celularevaluación de fármacospreparación de fármacoscell cultureevaluation of drugspreparation of drugs419 p.Mesenchymal stromal cells (MSCs) present the ability to secrete important immunomodulatory factors, and therefore, hold great potential for the treatment of multiple inflammatory and immune disorders. However, for the success of MSC-based therapies, the optimization of administration protocols is fundamental. In such regard, three-dimensional (3D) cell encapsulation emerges as a valuable strategy for MSC administration, since it overcomes the important hurdles of nude cell administration and represents a versatile platform to mimic the natural niche of cells. In the present doctoral thesis, we focused on the design of a multifunctional system consisting of a hydrogel incorporating alginate-poly-L-lysine-alginate (APA) microencapsulated MSCs, an adequate biofunctionalization strategy and the continuous presence of interferon-(IFN-), hypothesizing that it would represent an integrated solution to (1) protect MSCs from the host immune system, (2) to promote their viability and (3) to boost their immunomodulatory properties. Therefore, here, we studied in depth and optimized each one of the three main elements. In particular, we established a tight control on APA-microencapsulated MSC behavior enhancing the biosafety of the therapy, we designed a biosensor that provides essential information about the specific MSC biofunctionalization requirements and we developed a strategy to boost the immunomodulatory properties of MSCs based on the inclusion of IFN- and the 3D cell culture itself. These data represent an important step forward in the development of cell encapsulation systems, having significant implications in MSC therapies.Santos Vizcaíno, EdortaHernández Martín, Rosa María2020202020192019info:eu-repo/semantics/doctoralThesisapplication/pdfhttp://hdl.handle.net/10810/42317reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoInglésEuskerainfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/3.0/es/Atribución-NoComercial-CompartirIgual 3.0 España(cc)2019 AINHOA GONZALEZ PUJANA (cc by-nc-sa 4.0)oai:addi.ehu.eus:10810/423172026-06-18T09:23:17Z
dc.title.none.fl_str_mv Bioinspired three-dimensional multifunctional systems to enhance the therapeutic potential of mesenchymal stromal cells
Zelula estromal mesenkimalen eragin immunomodulatzailea sustatzeko bioinspiraturiko hiru dimentsiotako sistema funtzio anizdunak
title Bioinspired three-dimensional multifunctional systems to enhance the therapeutic potential of mesenchymal stromal cells
spellingShingle Bioinspired three-dimensional multifunctional systems to enhance the therapeutic potential of mesenchymal stromal cells
González Pujana, Ainhoa
cultivo celular
evaluación de fármacos
preparación de fármacos
cell culture
evaluation of drugs
preparation of drugs
title_short Bioinspired three-dimensional multifunctional systems to enhance the therapeutic potential of mesenchymal stromal cells
title_full Bioinspired three-dimensional multifunctional systems to enhance the therapeutic potential of mesenchymal stromal cells
title_fullStr Bioinspired three-dimensional multifunctional systems to enhance the therapeutic potential of mesenchymal stromal cells
title_full_unstemmed Bioinspired three-dimensional multifunctional systems to enhance the therapeutic potential of mesenchymal stromal cells
title_sort Bioinspired three-dimensional multifunctional systems to enhance the therapeutic potential of mesenchymal stromal cells
dc.creator.none.fl_str_mv González Pujana, Ainhoa
author González Pujana, Ainhoa
author_facet González Pujana, Ainhoa
author_role author
dc.contributor.none.fl_str_mv Santos Vizcaíno, Edorta
Hernández Martín, Rosa María
dc.subject.none.fl_str_mv cultivo celular
evaluación de fármacos
preparación de fármacos
cell culture
evaluation of drugs
preparation of drugs
topic cultivo celular
evaluación de fármacos
preparación de fármacos
cell culture
evaluation of drugs
preparation of drugs
description 419 p.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/42317
url http://hdl.handle.net/10810/42317
dc.language.none.fl_str_mv Inglés
Euskera
language_invalid_str_mv Inglés
Euskera
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/3.0/es/
Atribución-NoComercial-CompartirIgual 3.0 España
(cc)2019 AINHOA GONZALEZ PUJANA (cc by-nc-sa 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/3.0/es/
Atribución-NoComercial-CompartirIgual 3.0 España
(cc)2019 AINHOA GONZALEZ PUJANA (cc by-nc-sa 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869420098849079296
score 15,301603

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