Transcriptome expression analysis of candidate milk genes affecting cheese-related traits in 2 sheep breeds

[EN] Because ewe milk is principally used for cheese making, its quality is related to its content of total solids and the way in which milk constituents influence cheese yield and determine the technological and organoleptic characteristics of dairy products. Therefore, an in-depth knowledge of the...

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Autores: Suárez Vega, Aroa, Gutiérrez Gil, Beatriz, Arranz Santos, Juan José
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Universidad de León
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/24944
Acceso en línea:https://hdl.handle.net/10612/24944
Access Level:acceso abierto
Palabra clave:Genética
Producción animal
Veterinaria
Dairy sheep
RNA-Seq
Gene expression
Cheese traits
3109 Ciencias Veterinarias
3104 Producción Animal
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spelling Transcriptome expression analysis of candidate milk genes affecting cheese-related traits in 2 sheep breedsSuárez Vega, AroaGutiérrez Gil, BeatrizArranz Santos, Juan JoséGenéticaProducción animalVeterinariaDairy sheepRNA-SeqGene expressionCheese traits3109 Ciencias Veterinarias3104 Producción Animal[EN] Because ewe milk is principally used for cheese making, its quality is related to its content of total solids and the way in which milk constituents influence cheese yield and determine the technological and organoleptic characteristics of dairy products. Therefore, an in-depth knowledge of the expression levels of milk genes influencing cheese-related traits is essential. In the present study, the milk transcriptome data set of 2 dairy sheep breeds, Assaf and Spanish Churra, was used to evaluate the expression levels of 77 transcripts related to cheese yield and quality traits. For the comparison between both breeds, we selected the RNA sequencing (RNA-Seq) data at d 10 of lactation because this is the time point at which within and between breed differences due to lactation length are minimal. The evaluated genes encode major milk proteins (caseins and whey proteins), endogenous proteases, and enzymes related to fatty acid metabolism and citrate content. Through this analysis, we identified the genes predominantly expressed in each of the analyzed pathways that appear to be key genes for traits related to sheep milk cheese. Among the highly expressed genes in both breeds were the genes encoding caseins and whey proteins (CSN2, CSN3, CSN1S1, ENSOARG00000005099/PAEP, CSN1S2, LALBA), genes related to lipid metabolism (BTN1A1, XDH, FASN, ADFP, SCD, H-FABP, ACSS2), and one endogenous protease (CTSB). Moreover, a differential expression analysis between Churra and Assaf sheep allowed us to identify 7 genes that are significantly differentially expressed between the 2 breeds. These genes were mainly linked to endogenous protease activity (CTSL, CTSK, KLK10, KLK6, SERPINE2). Additionally, there were 2 differentially expressed genes coding for an intracellular fatty acid transporter (FABP4), an intermediate molecule of the citric acid cycle (SUCNR1), and 2 heat shock proteins (HSP70, HSPB8) that could be related to high protein production. The differential expression of these genes could have a direct influence on the different phenotypes observed between the 2 analyzed breedsSIThis work is included in the framework of the proj- ect AGL2012-34437 funded by the Spanish Ministry of Economy and Competitiveness (MINECO, Madrid, Spain). BGG is funded through the Spanish “Ramón y Cajal” Programme (RYC-2012-10230) from the MINE- CO. The support and availability of the computing facilities of the Foundation of Supercomputing Center of Castile and León (FCSCL, Leon, Spain; http://www. fcsc.es) are also greatly acknowledgedElsevier / American Dairy Science AssociationProducción AnimalFacultad de Veterinaria2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://hdl.handle.net/10612/24944reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad de LeónInglésinfo:eu-repo/grantAgreement/MINECO/Programa Nacional de Contratación e Incorporación/RYC-2012-10230http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/249442026-06-24T12:43:27Z
dc.title.none.fl_str_mv Transcriptome expression analysis of candidate milk genes affecting cheese-related traits in 2 sheep breeds
title Transcriptome expression analysis of candidate milk genes affecting cheese-related traits in 2 sheep breeds
spellingShingle Transcriptome expression analysis of candidate milk genes affecting cheese-related traits in 2 sheep breeds
Suárez Vega, Aroa
Genética
Producción animal
Veterinaria
Dairy sheep
RNA-Seq
Gene expression
Cheese traits
3109 Ciencias Veterinarias
3104 Producción Animal
title_short Transcriptome expression analysis of candidate milk genes affecting cheese-related traits in 2 sheep breeds
title_full Transcriptome expression analysis of candidate milk genes affecting cheese-related traits in 2 sheep breeds
title_fullStr Transcriptome expression analysis of candidate milk genes affecting cheese-related traits in 2 sheep breeds
title_full_unstemmed Transcriptome expression analysis of candidate milk genes affecting cheese-related traits in 2 sheep breeds
title_sort Transcriptome expression analysis of candidate milk genes affecting cheese-related traits in 2 sheep breeds
dc.creator.none.fl_str_mv Suárez Vega, Aroa
Gutiérrez Gil, Beatriz
Arranz Santos, Juan José
author Suárez Vega, Aroa
author_facet Suárez Vega, Aroa
Gutiérrez Gil, Beatriz
Arranz Santos, Juan José
author_role author
author2 Gutiérrez Gil, Beatriz
Arranz Santos, Juan José
author2_role author
author
dc.contributor.none.fl_str_mv Producción Animal
Facultad de Veterinaria
dc.subject.none.fl_str_mv Genética
Producción animal
Veterinaria
Dairy sheep
RNA-Seq
Gene expression
Cheese traits
3109 Ciencias Veterinarias
3104 Producción Animal
topic Genética
Producción animal
Veterinaria
Dairy sheep
RNA-Seq
Gene expression
Cheese traits
3109 Ciencias Veterinarias
3104 Producción Animal
description [EN] Because ewe milk is principally used for cheese making, its quality is related to its content of total solids and the way in which milk constituents influence cheese yield and determine the technological and organoleptic characteristics of dairy products. Therefore, an in-depth knowledge of the expression levels of milk genes influencing cheese-related traits is essential. In the present study, the milk transcriptome data set of 2 dairy sheep breeds, Assaf and Spanish Churra, was used to evaluate the expression levels of 77 transcripts related to cheese yield and quality traits. For the comparison between both breeds, we selected the RNA sequencing (RNA-Seq) data at d 10 of lactation because this is the time point at which within and between breed differences due to lactation length are minimal. The evaluated genes encode major milk proteins (caseins and whey proteins), endogenous proteases, and enzymes related to fatty acid metabolism and citrate content. Through this analysis, we identified the genes predominantly expressed in each of the analyzed pathways that appear to be key genes for traits related to sheep milk cheese. Among the highly expressed genes in both breeds were the genes encoding caseins and whey proteins (CSN2, CSN3, CSN1S1, ENSOARG00000005099/PAEP, CSN1S2, LALBA), genes related to lipid metabolism (BTN1A1, XDH, FASN, ADFP, SCD, H-FABP, ACSS2), and one endogenous protease (CTSB). Moreover, a differential expression analysis between Churra and Assaf sheep allowed us to identify 7 genes that are significantly differentially expressed between the 2 breeds. These genes were mainly linked to endogenous protease activity (CTSL, CTSK, KLK10, KLK6, SERPINE2). Additionally, there were 2 differentially expressed genes coding for an intracellular fatty acid transporter (FABP4), an intermediate molecule of the citric acid cycle (SUCNR1), and 2 heat shock proteins (HSP70, HSPB8) that could be related to high protein production. The differential expression of these genes could have a direct influence on the different phenotypes observed between the 2 analyzed breeds
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/10612/24944
url https://hdl.handle.net/10612/24944
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MINECO/Programa Nacional de Contratación e Incorporación/RYC-2012-10230
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier / American Dairy Science Association
publisher.none.fl_str_mv Elsevier / American Dairy Science Association
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad de León
instname_str Universidad de León
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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