Single DNA molecule detection in an optical trap using surface-enhanced Raman scattering
Raman spectra from single DNA molecules in their natural aqueous environment are presented. A DNA molecule that is anchored between two optically trapped dielectric beads is suspended in a solution with nanosized silvercolloid particles. The nonspecific binding of the metal to the DNA enhances the R...
| Autores: | , , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2010 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:115983 |
| Acceso en línea: | https://ddd.uab.cat/record/115983 https://dx.doi.org/urn:doi:10.1063/1.3431628 |
| Access Level: | acceso abierto |
| Palabra clave: | DNA Silver Colloidal systems Raman spectra Surface enhanced Raman scattering Metal surfaces Elasticity Metal optics Scattering measurements |
| Sumario: | Raman spectra from single DNA molecules in their natural aqueous environment are presented. A DNA molecule that is anchored between two optically trapped dielectric beads is suspended in a solution with nanosized silvercolloid particles. The nonspecific binding of the metal to the DNA enhances the Raman scattering that is excited by a near-infrared beam. A Raman spectrum is first recorded followed by a force-extension curve that verifies the presence of a single DNA molecule. |
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