Electrochemical immunosensing of Growth arrest-specific 6in human plasma and tumor cell secretomes

Growth arrest-specific 6 (GAS6) protein plays a key role in processes related to proliferation, inflammation, angiogenesis, and atherosclerotic plaque formation. In addition, it has been reported that plasma levels of GAS6 are related to cancer prognosis and other relevant pathologies, such as heart...

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Detalhes bibliográficos
Autores: Muñoz San Martín, Cristina, Pérez Ginés, Víctor, Torrente Rodríguez, Rebeca Magnolia, Gamella Carballo, María, Solís Fernández, Guillermo, Montero Calle, Ana, Pedrero Muñoz, María, Serafín González-Carrato, Verónica, Martínez Bosch, Neus, Navarro, Pilar, García de Frutos, Pablo, Batlle, Montserrat, Barderas Manchado, Rodrigo, Pingarrón Carrazón, José Manuel, Campuzano Ruiz, Susana
Tipo de documento: artigo
Data de publicação:2021
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositório:Docta Complutense
Idioma:inglês
OAI Identifier:oai:docta.ucm.es:20.500.14352/132184
Acesso em linha:https://hdl.handle.net/20.500.14352/132184
Access Level:Acceso aberto
Palavra-chave:543
Amperometric,
GAS6
Immunosensor
Plasma
Secretome
Ciencias
23 Química
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oai_identifier_str oai:docta.ucm.es:20.500.14352/132184
network_acronym_str ES
network_name_str España
repository_id_str
spelling Electrochemical immunosensing of Growth arrest-specific 6in human plasma and tumor cell secretomesMuñoz San Martín, CristinaPérez Ginés, VíctorTorrente Rodríguez, Rebeca MagnoliaGamella Carballo, MaríaSolís Fernández, GuillermoMontero Calle, AnaPedrero Muñoz, MaríaSerafín González-Carrato, VerónicaMartínez Bosch, NeusNavarro, PilarGarcía de Frutos, PabloBatlle, MontserratBarderas Manchado, RodrigoPingarrón Carrazón, José ManuelCampuzano Ruiz, Susana543Amperometric,GAS6ImmunosensorPlasmaSecretomeCiencias23 QuímicaGrowth arrest-specific 6 (GAS6) protein plays a key role in processes related to proliferation, inflammation, angiogenesis, and atherosclerotic plaque formation. In addition, it has been reported that plasma levels of GAS6 are related to cancer prognosis and other relevant pathologies, such as heart failure or sepsis. We report here the first electrochemical immunoplatform for the determination of GAS6, which has demonstrated to be competitive with other available methodologies in terms of cost, simplicity, and decentralized application. The developed immunoplatform involves a sandwich immunoassay using magnetic microparticles (MBs) and uses amperometric detection at disposable screen-printed carbon electrodes (SPCEs). The MBs were modified with an antibody specific to GAS6 for its selective capture, which is further recognized by a biotinylated secondary antibody subsequently labeled with a streptavidin-horseradish peroxidase (Strep-HRP) conjugate. The electrochemical detection was carried out using the hydroquinone (HQ)/H2O2 system. The developed bioplatform exhibits a great selectivity and low limit of detection (27 pg/mL) that allowed the determination of the GAS6 circulating level in plasma samples from patients suffering heart failure (HF) and diagnosed with pancreatic ductal adenocarcinoma (PDAC), as well as the determination of the target protein in raw secretomes of human colorectal cancer cell lines.WileyUniversidad Complutense de Madrid20212021-07-0920212021-07-09journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/132184reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1321842026-06-02T12:44:21Z
dc.title.none.fl_str_mv Electrochemical immunosensing of Growth arrest-specific 6in human plasma and tumor cell secretomes
title Electrochemical immunosensing of Growth arrest-specific 6in human plasma and tumor cell secretomes
spellingShingle Electrochemical immunosensing of Growth arrest-specific 6in human plasma and tumor cell secretomes
Muñoz San Martín, Cristina
543
Amperometric,
GAS6
Immunosensor
Plasma
Secretome
Ciencias
23 Química
title_short Electrochemical immunosensing of Growth arrest-specific 6in human plasma and tumor cell secretomes
title_full Electrochemical immunosensing of Growth arrest-specific 6in human plasma and tumor cell secretomes
title_fullStr Electrochemical immunosensing of Growth arrest-specific 6in human plasma and tumor cell secretomes
title_full_unstemmed Electrochemical immunosensing of Growth arrest-specific 6in human plasma and tumor cell secretomes
title_sort Electrochemical immunosensing of Growth arrest-specific 6in human plasma and tumor cell secretomes
dc.creator.none.fl_str_mv Muñoz San Martín, Cristina
Pérez Ginés, Víctor
Torrente Rodríguez, Rebeca Magnolia
Gamella Carballo, María
Solís Fernández, Guillermo
Montero Calle, Ana
Pedrero Muñoz, María
Serafín González-Carrato, Verónica
Martínez Bosch, Neus
Navarro, Pilar
García de Frutos, Pablo
Batlle, Montserrat
Barderas Manchado, Rodrigo
Pingarrón Carrazón, José Manuel
Campuzano Ruiz, Susana
author Muñoz San Martín, Cristina
author_facet Muñoz San Martín, Cristina
Pérez Ginés, Víctor
Torrente Rodríguez, Rebeca Magnolia
Gamella Carballo, María
Solís Fernández, Guillermo
Montero Calle, Ana
Pedrero Muñoz, María
Serafín González-Carrato, Verónica
Martínez Bosch, Neus
Navarro, Pilar
García de Frutos, Pablo
Batlle, Montserrat
Barderas Manchado, Rodrigo
Pingarrón Carrazón, José Manuel
Campuzano Ruiz, Susana
author_role author
author2 Pérez Ginés, Víctor
Torrente Rodríguez, Rebeca Magnolia
Gamella Carballo, María
Solís Fernández, Guillermo
Montero Calle, Ana
Pedrero Muñoz, María
Serafín González-Carrato, Verónica
Martínez Bosch, Neus
Navarro, Pilar
García de Frutos, Pablo
Batlle, Montserrat
Barderas Manchado, Rodrigo
Pingarrón Carrazón, José Manuel
Campuzano Ruiz, Susana
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 543
Amperometric,
GAS6
Immunosensor
Plasma
Secretome
Ciencias
23 Química
topic 543
Amperometric,
GAS6
Immunosensor
Plasma
Secretome
Ciencias
23 Química
description Growth arrest-specific 6 (GAS6) protein plays a key role in processes related to proliferation, inflammation, angiogenesis, and atherosclerotic plaque formation. In addition, it has been reported that plasma levels of GAS6 are related to cancer prognosis and other relevant pathologies, such as heart failure or sepsis. We report here the first electrochemical immunoplatform for the determination of GAS6, which has demonstrated to be competitive with other available methodologies in terms of cost, simplicity, and decentralized application. The developed immunoplatform involves a sandwich immunoassay using magnetic microparticles (MBs) and uses amperometric detection at disposable screen-printed carbon electrodes (SPCEs). The MBs were modified with an antibody specific to GAS6 for its selective capture, which is further recognized by a biotinylated secondary antibody subsequently labeled with a streptavidin-horseradish peroxidase (Strep-HRP) conjugate. The electrochemical detection was carried out using the hydroquinone (HQ)/H2O2 system. The developed bioplatform exhibits a great selectivity and low limit of detection (27 pg/mL) that allowed the determination of the GAS6 circulating level in plasma samples from patients suffering heart failure (HF) and diagnosed with pancreatic ductal adenocarcinoma (PDAC), as well as the determination of the target protein in raw secretomes of human colorectal cancer cell lines.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-07-09
2021
2021-07-09
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/132184
url https://hdl.handle.net/20.500.14352/132184
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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