Almond (Prunus dulcis (Mill.) D.A. Webb) polyphenols: From chemical characterization to targeted analysis of phenolic metabolites in humans

In this paper, a survey of our studies on almond polyphenols including their chemical characterization and further bioavailability in humans is reported. Combination of analytical techniques (LC-DAD/fluorescence, LC/ESI-MS and MALDI-TOF-MS) allowed us, for the first time, the identification of A- an...

Full description

Bibliographic Details
Authors: Bartolomé, Begoña, Monagas Juan, María Josefina, Garrido, Ignacio, Gómez-Cordovés, Carmen, Martín-Álvarez, Pedro J., Lebrón-Aguilar, Rosa, Urpí-Sarda, Mireia, Llorach, Rafael, Andrés-Lacueva, Cristina
Format: article
Status:Versión aceptada para publicación
Publication Date:2010
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/49667
Online Access:http://hdl.handle.net/10261/49667
Access Level:Open access
Keyword:Almond skins
Flavan-3-ols
Conjugated phenolic metabolites
Microbial-derived phenolic metabolites
Hydroxyphenylvalerolactones
Targeted analysis
Description
Summary:In this paper, a survey of our studies on almond polyphenols including their chemical characterization and further bioavailability in humans is reported. Combination of analytical techniques (LC-DAD/fluorescence, LC/ESI-MS and MALDI-TOF-MS) allowed us, for the first time, the identification of A- and B-type procyanidin, propelargonidin and prodelphinidin polymers in almond skins. Glucuronide, O-methyl glucuronide, sulfate and O-methyl sulfate derivatives of (epi)catechin, as well as the glucuronide conjugates of naringenin and isorhamnetin, and sulfate conjugates of isorhamnetin, together with conjugates of hydroxyphenylvalerolactones were detected in plasma and urine samples after the intake of almond skin polyphenols. In addition, numerous microbial-derived metabolites, including hydroxyphenylpropionic, hydroxyphenylacetic, hydroxycinnamic, hydroxybenzoic and hydroxyhippuric acids were also identified. Depending of the type of metabolite, maximum urinary excretion was attained at different time in comparison to the control group in the course of the 24-h period of urine excretion, allowing us to establish the onset of microbial metabolism.

Similar Items