Long-Term Effects Following Fresh/Vitrified Embryo Transfer Are Transmitted by Paternal Germline in a Large Size Rabbit Cohort

[EN] Simple Summary Assisted reproductive technologies (ARTs) involve an extraordinary change in the natural developmental trajectory of the mammalian embryo, incurring potential long-term and inheritable effects in the resulting offspring. The results of this study demonstrate, for the first time,...

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Detalles Bibliográficos
Autores: Garcia-Dominguez, X, Viudes-de-Castro, Maria P., Vicente Antón, José Salvador|||0000-0003-0481-3802, Marco-Jiménez, Francisco|||0000-0002-5226-4118
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Ajuntament de Barcelona
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/176262
Acceso en línea:https://riunet.upv.es/handle/10251/176262
Access Level:acceso abierto
Palabra clave:Assisted reproduction technology
Paternal inheritance
Maternal inheritance
Embryo transfer
Embryo cryopreservation
Long-term effects
Developmental plasticity
Developmental programming
BIOLOGIA ANIMAL
PRODUCCION ANIMAL
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spelling Long-Term Effects Following Fresh/Vitrified Embryo Transfer Are Transmitted by Paternal Germline in a Large Size Rabbit CohortGarcia-Dominguez, XViudes-de-Castro, Maria P.Vicente Antón, José Salvador|||0000-0003-0481-3802Marco-Jiménez, Francisco|||0000-0002-5226-4118Assisted reproduction technologyPaternal inheritanceMaternal inheritanceEmbryo transferEmbryo cryopreservationLong-term effectsDevelopmental plasticityDevelopmental programmingBIOLOGIA ANIMALPRODUCCION ANIMAL[EN] Simple Summary Assisted reproductive technologies (ARTs) involve an extraordinary change in the natural developmental trajectory of the mammalian embryo, incurring potential long-term and inheritable effects in the resulting offspring. The results of this study demonstrate, for the first time, that ex vivo embryo manipulations during fresh and vitrified embryo transfer are associated with paternally inherited bodyweight variation, but seemed not transmissible via the female germline. This asymmetry in the transmission of acquired features following ARTs suggests that embryo paternal and maternal genomes differ in their degree of susceptibility to the lasting effects of ARTs. This study would provide a novel view of developmental plasticity in the early mammalian embryo. The concept of developmental programming suggests that the early life environment influences offspring phenotype in later life, whose effects may also be manifested in further generations. Valuable pieces of evidence come from the fields applying assisted reproductive technologies (ARTs), which deprive embryos of their optimal maternal environment and were thus associated with subsequent developmental deviations. Recently, we demonstrated that the in vitro manipulations during a vitrified embryo transfer procedure incurs a cumulative and transgenerational decline in the growth performance of the resulting offspring. Here, we provide a longitudinal study to investigate whether previous developmental deviations could be indistinctly paternally or maternally transmitted using crossbred mattings. Our findings revealed that early embryo manipulations through fresh and vitrified embryo transfer incurred paternally transmissible effects over the growth pattern and adult body weight, which seemed not inheritable via the female germline. Similar inheritable effects were observed after fresh and vitrified embryo transfer, suggesting that disturbing optimal embryo development through in vitro manipulations was the principal trigger of transmissible effects, rather than embryo cryopreservation per se.This research was funded by Conselleria d'Educacio, Investigacio, Cultura i Esport (Generalitat Valenciana, Spain), grant number AICO/2019/272. X.G.-D. was supported by a research grant from the Spanish Ministry of Economy, Industry, and Competitiveness, grant number BES-2015-072429.MDPI AGDepartamento de Ciencia AnimalInstituto Universitario de Ciencia y Tecnología AnimalEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalMinisterio de Economía y EmpresaGeneralitat ValencianaRepositorio Institucional de la Universitat Politècnica de València Riunet20202020-07-25journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/176262reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Ajuntament de BarcelonaInglésengMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 BES-2015-072429 BES-2015-072429Generalitat Valenciana https://doi.org/10.13039/501100003359 AICO%2F2019%2F272 Cambios en el genoma y su impacto fenotípico en una línea maternal de conejos tras sucesivas aplicaciones de un programa de crioconservación.open accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/1762622026-06-13T07:49:27Z
dc.title.none.fl_str_mv Long-Term Effects Following Fresh/Vitrified Embryo Transfer Are Transmitted by Paternal Germline in a Large Size Rabbit Cohort
title Long-Term Effects Following Fresh/Vitrified Embryo Transfer Are Transmitted by Paternal Germline in a Large Size Rabbit Cohort
spellingShingle Long-Term Effects Following Fresh/Vitrified Embryo Transfer Are Transmitted by Paternal Germline in a Large Size Rabbit Cohort
Garcia-Dominguez, X
Assisted reproduction technology
Paternal inheritance
Maternal inheritance
Embryo transfer
Embryo cryopreservation
Long-term effects
Developmental plasticity
Developmental programming
BIOLOGIA ANIMAL
PRODUCCION ANIMAL
title_short Long-Term Effects Following Fresh/Vitrified Embryo Transfer Are Transmitted by Paternal Germline in a Large Size Rabbit Cohort
title_full Long-Term Effects Following Fresh/Vitrified Embryo Transfer Are Transmitted by Paternal Germline in a Large Size Rabbit Cohort
title_fullStr Long-Term Effects Following Fresh/Vitrified Embryo Transfer Are Transmitted by Paternal Germline in a Large Size Rabbit Cohort
title_full_unstemmed Long-Term Effects Following Fresh/Vitrified Embryo Transfer Are Transmitted by Paternal Germline in a Large Size Rabbit Cohort
title_sort Long-Term Effects Following Fresh/Vitrified Embryo Transfer Are Transmitted by Paternal Germline in a Large Size Rabbit Cohort
dc.creator.none.fl_str_mv Garcia-Dominguez, X
Viudes-de-Castro, Maria P.
Vicente Antón, José Salvador|||0000-0003-0481-3802
Marco-Jiménez, Francisco|||0000-0002-5226-4118
author Garcia-Dominguez, X
author_facet Garcia-Dominguez, X
Viudes-de-Castro, Maria P.
Vicente Antón, José Salvador|||0000-0003-0481-3802
Marco-Jiménez, Francisco|||0000-0002-5226-4118
author_role author
author2 Viudes-de-Castro, Maria P.
Vicente Antón, José Salvador|||0000-0003-0481-3802
Marco-Jiménez, Francisco|||0000-0002-5226-4118
author2_role author
author
author
dc.contributor.none.fl_str_mv Departamento de Ciencia Animal
Instituto Universitario de Ciencia y Tecnología Animal
Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural
Ministerio de Economía y Empresa
Generalitat Valenciana
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Assisted reproduction technology
Paternal inheritance
Maternal inheritance
Embryo transfer
Embryo cryopreservation
Long-term effects
Developmental plasticity
Developmental programming
BIOLOGIA ANIMAL
PRODUCCION ANIMAL
topic Assisted reproduction technology
Paternal inheritance
Maternal inheritance
Embryo transfer
Embryo cryopreservation
Long-term effects
Developmental plasticity
Developmental programming
BIOLOGIA ANIMAL
PRODUCCION ANIMAL
description [EN] Simple Summary Assisted reproductive technologies (ARTs) involve an extraordinary change in the natural developmental trajectory of the mammalian embryo, incurring potential long-term and inheritable effects in the resulting offspring. The results of this study demonstrate, for the first time, that ex vivo embryo manipulations during fresh and vitrified embryo transfer are associated with paternally inherited bodyweight variation, but seemed not transmissible via the female germline. This asymmetry in the transmission of acquired features following ARTs suggests that embryo paternal and maternal genomes differ in their degree of susceptibility to the lasting effects of ARTs. This study would provide a novel view of developmental plasticity in the early mammalian embryo. The concept of developmental programming suggests that the early life environment influences offspring phenotype in later life, whose effects may also be manifested in further generations. Valuable pieces of evidence come from the fields applying assisted reproductive technologies (ARTs), which deprive embryos of their optimal maternal environment and were thus associated with subsequent developmental deviations. Recently, we demonstrated that the in vitro manipulations during a vitrified embryo transfer procedure incurs a cumulative and transgenerational decline in the growth performance of the resulting offspring. Here, we provide a longitudinal study to investigate whether previous developmental deviations could be indistinctly paternally or maternally transmitted using crossbred mattings. Our findings revealed that early embryo manipulations through fresh and vitrified embryo transfer incurred paternally transmissible effects over the growth pattern and adult body weight, which seemed not inheritable via the female germline. Similar inheritable effects were observed after fresh and vitrified embryo transfer, suggesting that disturbing optimal embryo development through in vitro manipulations was the principal trigger of transmissible effects, rather than embryo cryopreservation per se.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-07-25
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/176262
url https://riunet.upv.es/handle/10251/176262
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 BES-2015-072429 BES-2015-072429
Generalitat Valenciana https://doi.org/10.13039/501100003359 AICO%2F2019%2F272 Cambios en el genoma y su impacto fenotípico en una línea maternal de conejos tras sucesivas aplicaciones de un programa de crioconservación.
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Ajuntament de Barcelona
instname_str Ajuntament de Barcelona
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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