Antimicrobial Resistance Genes in Porcine Pasteurella multocida Are Not Associated with Its Antimicrobial Susceptibility Pattern

Forty-eight Pasteurella multocida isolates were recovered from porcine pneumonic lungs collected from farms in "Castilla y León" (north-western Spain) in 2017-2019. These isolates were characterized for their minimal inhibition concentrations to twelve antimicrobial agents and for the appe...

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Authors: Patrocchi-Rilo, Máximo, Gutiérrez-Martín, César-B., Pérez-Fernández, Esther, Vilaró, Anna, Fraile Sauce, Lorenzo José, Martínez, Sonia
Format: article
Status:Published version
Publication Date:2020
Country:España
Institution:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repository:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10459.1/69651
Online Access:https://doi.org/10.3390/antibiotics9090614
http://hdl.handle.net/10459.1/69651
Access Level:Open access
Keyword:Pasteurella multocida
Antimicrobial resistance genes
Antimicrobial susceptibility patterns
Swine
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spelling Antimicrobial Resistance Genes in Porcine Pasteurella multocida Are Not Associated with Its Antimicrobial Susceptibility PatternPatrocchi-Rilo, MáximoGutiérrez-Martín, César-B.Pérez-Fernández, EstherVilaró, AnnaFraile Sauce, Lorenzo JoséMartínez, SoniaPasteurella multocidaAntimicrobial resistance genesAntimicrobial susceptibility patternsSwineForty-eight Pasteurella multocida isolates were recovered from porcine pneumonic lungs collected from farms in "Castilla y León" (north-western Spain) in 2017-2019. These isolates were characterized for their minimal inhibition concentrations to twelve antimicrobial agents and for the appearance of eight resistance genes: tetA, tetB, blaROB1, blaTEM, ermA, ermC, mphE and msrE. Relevant resistance percentages were shown against tetracyclines (52.1% for doxycycline, 68.7% for oxytetracycline), sulphamethoxazole/trimethoprim (43.7%) and tiamulin (25.0%), thus suggesting that P. multocida isolates were mostly susceptible to amoxicillin, ceftiofur, enrofloxacin, florfenicol, marbofloxacin and macrolides. Overall, 29.2% of isolates were resistant to more than two antimicrobials. The tetracycline resistance genes (tetA and tetB) were detected in 22.9% of the isolates, but none were positive to both simultaneously; blaROB1 and blaTEM genes were found in one third of isolates but both genes were detected simultaneously in only one isolate. The ermC gene was observed in 41.7% of isolates, a percentage that decreased to 22.9% for msrE; finally, ermA was harbored by 16.7% and mphE was not found in any of them. Six clusters were established based on hierarchical clustering analysis on antimicrobial susceptibility for the twelve antimicrobials. Generally, it was unable to foresee the antimicrobial susceptibility pattern for each family and the association of each particular isolate inside the clusters established from the presence or absence of the resistance genes analyzed.Junta de Castilla y León (Consejería de Agricultura y Ganadería, Junta de Castilla y León, Spain) and Cost Action CA18217 (European Network for Optimization of Veterinary Antimicrobial Treatment).MDPI2020202020202020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://doi.org/10.3390/antibiotics9090614http://hdl.handle.net/10459.1/69651http://hdl.handle.net/10459.1/69651reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.3390/antibiotics9090614Antibiotics-Basel, 2020, vol. 9, num. 9, p. 614cc-by (c) Patrocchi-Rilo, Máximo et al., 2020info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/oai:recercat.cat:10459.1/696512026-05-29T05:05:01Z
dc.title.none.fl_str_mv Antimicrobial Resistance Genes in Porcine Pasteurella multocida Are Not Associated with Its Antimicrobial Susceptibility Pattern
title Antimicrobial Resistance Genes in Porcine Pasteurella multocida Are Not Associated with Its Antimicrobial Susceptibility Pattern
spellingShingle Antimicrobial Resistance Genes in Porcine Pasteurella multocida Are Not Associated with Its Antimicrobial Susceptibility Pattern
Patrocchi-Rilo, Máximo
Pasteurella multocida
Antimicrobial resistance genes
Antimicrobial susceptibility patterns
Swine
title_short Antimicrobial Resistance Genes in Porcine Pasteurella multocida Are Not Associated with Its Antimicrobial Susceptibility Pattern
title_full Antimicrobial Resistance Genes in Porcine Pasteurella multocida Are Not Associated with Its Antimicrobial Susceptibility Pattern
title_fullStr Antimicrobial Resistance Genes in Porcine Pasteurella multocida Are Not Associated with Its Antimicrobial Susceptibility Pattern
title_full_unstemmed Antimicrobial Resistance Genes in Porcine Pasteurella multocida Are Not Associated with Its Antimicrobial Susceptibility Pattern
title_sort Antimicrobial Resistance Genes in Porcine Pasteurella multocida Are Not Associated with Its Antimicrobial Susceptibility Pattern
dc.creator.none.fl_str_mv Patrocchi-Rilo, Máximo
Gutiérrez-Martín, César-B.
Pérez-Fernández, Esther
Vilaró, Anna
Fraile Sauce, Lorenzo José
Martínez, Sonia
author Patrocchi-Rilo, Máximo
author_facet Patrocchi-Rilo, Máximo
Gutiérrez-Martín, César-B.
Pérez-Fernández, Esther
Vilaró, Anna
Fraile Sauce, Lorenzo José
Martínez, Sonia
author_role author
author2 Gutiérrez-Martín, César-B.
Pérez-Fernández, Esther
Vilaró, Anna
Fraile Sauce, Lorenzo José
Martínez, Sonia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Pasteurella multocida
Antimicrobial resistance genes
Antimicrobial susceptibility patterns
Swine
topic Pasteurella multocida
Antimicrobial resistance genes
Antimicrobial susceptibility patterns
Swine
description Forty-eight Pasteurella multocida isolates were recovered from porcine pneumonic lungs collected from farms in "Castilla y León" (north-western Spain) in 2017-2019. These isolates were characterized for their minimal inhibition concentrations to twelve antimicrobial agents and for the appearance of eight resistance genes: tetA, tetB, blaROB1, blaTEM, ermA, ermC, mphE and msrE. Relevant resistance percentages were shown against tetracyclines (52.1% for doxycycline, 68.7% for oxytetracycline), sulphamethoxazole/trimethoprim (43.7%) and tiamulin (25.0%), thus suggesting that P. multocida isolates were mostly susceptible to amoxicillin, ceftiofur, enrofloxacin, florfenicol, marbofloxacin and macrolides. Overall, 29.2% of isolates were resistant to more than two antimicrobials. The tetracycline resistance genes (tetA and tetB) were detected in 22.9% of the isolates, but none were positive to both simultaneously; blaROB1 and blaTEM genes were found in one third of isolates but both genes were detected simultaneously in only one isolate. The ermC gene was observed in 41.7% of isolates, a percentage that decreased to 22.9% for msrE; finally, ermA was harbored by 16.7% and mphE was not found in any of them. Six clusters were established based on hierarchical clustering analysis on antimicrobial susceptibility for the twelve antimicrobials. Generally, it was unable to foresee the antimicrobial susceptibility pattern for each family and the association of each particular isolate inside the clusters established from the presence or absence of the resistance genes analyzed.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.3390/antibiotics9090614
http://hdl.handle.net/10459.1/69651
http://hdl.handle.net/10459.1/69651
url https://doi.org/10.3390/antibiotics9090614
http://hdl.handle.net/10459.1/69651
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/antibiotics9090614
Antibiotics-Basel, 2020, vol. 9, num. 9, p. 614
dc.rights.none.fl_str_mv cc-by (c) Patrocchi-Rilo, Máximo et al., 2020
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv cc-by (c) Patrocchi-Rilo, Máximo et al., 2020
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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