Gene expression following induction of regeneration in Drosophila wing imaginal discs

Background: Regeneration is the ability of an organism to rebuild a body part that has been damaged or amputated, and can be studied at the molecular level using model organisms. Drosophila imaginal discs, which are the larval primordia of adult cuticular structures, are capable of undergoing regene...

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Autores: Blanco, Enrique, Ruiz Romero, Marina, Beltran i Agulló, Sergi, Bosch Marimon, Manel, Punset, Adrià, Serras Rigalt, Florenci, Corominas, Montserrat (Corominas Guiu)
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2010
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/13752
Acceso en línea:https://hdl.handle.net/2445/13752
Access Level:acceso abierto
Palabra clave:Expressió gènica
Drosòfila
Gene expression
Drosophila
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spelling Gene expression following induction of regeneration in Drosophila wing imaginal discsBlanco, EnriqueRuiz Romero, MarinaBeltran i Agulló, SergiBosch Marimon, ManelPunset, AdriàSerras Rigalt, FlorenciCorominas, Montserrat (Corominas Guiu)Expressió gènicaDrosòfilaGene expressionDrosophilaBackground: Regeneration is the ability of an organism to rebuild a body part that has been damaged or amputated, and can be studied at the molecular level using model organisms. Drosophila imaginal discs, which are the larval primordia of adult cuticular structures, are capable of undergoing regenerative growth after transplantation and in vivo culture into the adult abdomen. Results: Using expression profile analyses, we studied the regenerative behaviour of wing discs at 0, 24 and 72 hours after fragmentation and implantation into adult females. Based on expression level, we generated a catalogue of genes with putative role in wing disc regeneration, identifying four classes: 1) genes with differential expression within the first 24 hours; 2) genes with differential expression between 24 and 72 hours; 3) genes that changed significantly in expression levels between the two time periods; 4) genes with a sustained increase or decrease in their expression levels throughout regeneration. Among these genes, we identified members of the JNK and Notch signalling pathways and chromatin regulators. Through computational analysis, we recognized putative binding sites for transcription factors downstream of these pathways that are conserved in multiple Drosophilids, indicating a potential relationship between members of the different gene classes. Experimental data from genetic mutants provide evidence of a requirement of selected genes in wing disc regeneration. Conclusions: We have been able to distinguish various classes of genes involved in early and late steps of the regeneration process. Our data suggests the integration of signalling pathways in the promoters of regulated genes.BioMed Central201020102010info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion14 p.application/pdfhttps://hdl.handle.net/2445/13752Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a http://dx.doi.org/10.1186/1471-213X-10-94BMC Developmental Biology 2010, 10:94http://dx.doi.org/10.1186/1471-213X-10-94cc-by, (c) Blanco et al., 2010http://creativecommons.org/licenses/by/2.0info:eu-repo/semantics/openAccessoai:recercat.cat:2445/137522026-05-29T05:05:01Z
dc.title.none.fl_str_mv Gene expression following induction of regeneration in Drosophila wing imaginal discs
title Gene expression following induction of regeneration in Drosophila wing imaginal discs
spellingShingle Gene expression following induction of regeneration in Drosophila wing imaginal discs
Blanco, Enrique
Expressió gènica
Drosòfila
Gene expression
Drosophila
title_short Gene expression following induction of regeneration in Drosophila wing imaginal discs
title_full Gene expression following induction of regeneration in Drosophila wing imaginal discs
title_fullStr Gene expression following induction of regeneration in Drosophila wing imaginal discs
title_full_unstemmed Gene expression following induction of regeneration in Drosophila wing imaginal discs
title_sort Gene expression following induction of regeneration in Drosophila wing imaginal discs
dc.creator.none.fl_str_mv Blanco, Enrique
Ruiz Romero, Marina
Beltran i Agulló, Sergi
Bosch Marimon, Manel
Punset, Adrià
Serras Rigalt, Florenci
Corominas, Montserrat (Corominas Guiu)
author Blanco, Enrique
author_facet Blanco, Enrique
Ruiz Romero, Marina
Beltran i Agulló, Sergi
Bosch Marimon, Manel
Punset, Adrià
Serras Rigalt, Florenci
Corominas, Montserrat (Corominas Guiu)
author_role author
author2 Ruiz Romero, Marina
Beltran i Agulló, Sergi
Bosch Marimon, Manel
Punset, Adrià
Serras Rigalt, Florenci
Corominas, Montserrat (Corominas Guiu)
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Expressió gènica
Drosòfila
Gene expression
Drosophila
topic Expressió gènica
Drosòfila
Gene expression
Drosophila
description Background: Regeneration is the ability of an organism to rebuild a body part that has been damaged or amputated, and can be studied at the molecular level using model organisms. Drosophila imaginal discs, which are the larval primordia of adult cuticular structures, are capable of undergoing regenerative growth after transplantation and in vivo culture into the adult abdomen. Results: Using expression profile analyses, we studied the regenerative behaviour of wing discs at 0, 24 and 72 hours after fragmentation and implantation into adult females. Based on expression level, we generated a catalogue of genes with putative role in wing disc regeneration, identifying four classes: 1) genes with differential expression within the first 24 hours; 2) genes with differential expression between 24 and 72 hours; 3) genes that changed significantly in expression levels between the two time periods; 4) genes with a sustained increase or decrease in their expression levels throughout regeneration. Among these genes, we identified members of the JNK and Notch signalling pathways and chromatin regulators. Through computational analysis, we recognized putative binding sites for transcription factors downstream of these pathways that are conserved in multiple Drosophilids, indicating a potential relationship between members of the different gene classes. Experimental data from genetic mutants provide evidence of a requirement of selected genes in wing disc regeneration. Conclusions: We have been able to distinguish various classes of genes involved in early and late steps of the regeneration process. Our data suggests the integration of signalling pathways in the promoters of regulated genes.
publishDate 2010
dc.date.none.fl_str_mv 2010
2010
2010
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/13752
url https://hdl.handle.net/2445/13752
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a http://dx.doi.org/10.1186/1471-213X-10-94
BMC Developmental Biology 2010, 10:94
http://dx.doi.org/10.1186/1471-213X-10-94
dc.rights.none.fl_str_mv cc-by, (c) Blanco et al., 2010
http://creativecommons.org/licenses/by/2.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by, (c) Blanco et al., 2010
http://creativecommons.org/licenses/by/2.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 14 p.
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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