Cryobanking European Mink (Mustela lutreola) Mesenchymal Stem Cells and Oocytes

12 Pág.

Detalles Bibliográficos
Autores: Calle, Alexandra, Ramírez, Miguel Ángel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/304807
Acceso en línea:http://hdl.handle.net/10261/304807
https://api.elsevier.com/content/abstract/scopus_id/85137124460
Access Level:acceso abierto
Palabra clave:European mink
Biobank
Ex situ
Conservation
Mesenchymal stem/stromal cells
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spelling Cryobanking European Mink (Mustela lutreola) Mesenchymal Stem Cells and OocytesCalle, AlexandraRamírez, Miguel ÁngelEuropean minkBiobankEx situConservationMesenchymal stem/stromal cells12 Pág.The European mink (Mustela lutreola) is one of Europe's most endangered species, and it is on the brink of extinction in the Iberian Peninsula. The species' precarious situation requires the application of new ex situ conservation methodologies that complement the existing ex situ and in situ conservation measures. Here, we report for the first time the establishment of a biobank for European mink mesenchymal stem cells (emMSC) and oocytes from specimens found dead in the Iberian Peninsula, either free or in captivity. New emMSC lines were isolated from different tissues: bone marrow (emBM-MSC), oral mucosa (emOM-MSc), dermal skin (emDS-MSC), oviduct (emO-MSc), endometrium (emE-MSC), testicular (emT-MSC), and adipose tissue from two different adipose depots: subcutaneous (emSCA-MSC) and ovarian (emOA-MSC). All eight emMSC lines showed plastic adhesion, a detectable expression of characteristic markers of MSCs, and, when cultured under osteogenic and adipogenic conditions, differentiation capacity to these lineages. Additionally, we were able to keep 227 Cumulus-oocyte complexes (COCs) in the biobank, 97 of which are grade I or II. The European mink MSC and oocyte biobank will allow for the conservation of the species' genetic variability, the application of assisted reproduction techniques, and the development of in vitro models for studying the molecular mechanisms of infectious diseases that threaten the species' precarious situation.This work was supported by grant PCRV00820 funded by MITECO and Fundación Biodiversidad, European Union’s Horizon 2020 Research and Innovation program VetBioNet (grant INFRAIA-731014), and grant PID2019-107145RB-I00 funded by MCIN/AEI/10.13039/501100011033Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio para la Transición Ecológica y el Reto Demográfico (España)Fundación BiodiversidadEuropean CommissionMinisterio de Ciencia e Innovación (España)Calle, Alexandra [0000-0003-3903-5019]Ramírez, Miguel Ángel [0000-0002-5868-2134]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/304807https://api.elsevier.com/content/abstract/scopus_id/85137124460reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/H2020/info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-107145RB-I00Departamento de Reproducción Animal​ https://doi.org/10.3390/ijms23169319Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3048072026-05-22T06:33:51Z
dc.title.none.fl_str_mv Cryobanking European Mink (Mustela lutreola) Mesenchymal Stem Cells and Oocytes
title Cryobanking European Mink (Mustela lutreola) Mesenchymal Stem Cells and Oocytes
spellingShingle Cryobanking European Mink (Mustela lutreola) Mesenchymal Stem Cells and Oocytes
Calle, Alexandra
European mink
Biobank
Ex situ
Conservation
Mesenchymal stem/stromal cells
title_short Cryobanking European Mink (Mustela lutreola) Mesenchymal Stem Cells and Oocytes
title_full Cryobanking European Mink (Mustela lutreola) Mesenchymal Stem Cells and Oocytes
title_fullStr Cryobanking European Mink (Mustela lutreola) Mesenchymal Stem Cells and Oocytes
title_full_unstemmed Cryobanking European Mink (Mustela lutreola) Mesenchymal Stem Cells and Oocytes
title_sort Cryobanking European Mink (Mustela lutreola) Mesenchymal Stem Cells and Oocytes
dc.creator.none.fl_str_mv Calle, Alexandra
Ramírez, Miguel Ángel
author Calle, Alexandra
author_facet Calle, Alexandra
Ramírez, Miguel Ángel
author_role author
author2 Ramírez, Miguel Ángel
author2_role author
dc.contributor.none.fl_str_mv Ministerio para la Transición Ecológica y el Reto Demográfico (España)
Fundación Biodiversidad
European Commission
Ministerio de Ciencia e Innovación (España)
Calle, Alexandra [0000-0003-3903-5019]
Ramírez, Miguel Ángel [0000-0002-5868-2134]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv European mink
Biobank
Ex situ
Conservation
Mesenchymal stem/stromal cells
topic European mink
Biobank
Ex situ
Conservation
Mesenchymal stem/stromal cells
description 12 Pág.
publishDate 2022
dc.date.none.fl_str_mv 2022
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/304807
https://api.elsevier.com/content/abstract/scopus_id/85137124460
url http://hdl.handle.net/10261/304807
https://api.elsevier.com/content/abstract/scopus_id/85137124460
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/H2020/
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-107145RB-I00
Departamento de Reproducción Animal​ 
https://doi.org/10.3390/ijms23169319
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
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