Brain and immune system-derived extracellular vesicles mediate regulation of complement system, extracellular matrix remodeling, brain repair and antigen tolerance in Multiple sclerosis
Background: Multiple sclerosis (MS) is an immune-mediated central nervous system disease whose course is unpredictable. Finding biomarkers that help to better comprehend the disease's pathogenesis is crucial for supporting clinical decision-making. Blood extracellular vesicles (EVs) are membran...
| Autores: | , , , , , , , , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Servizo Galego de Saúde (SERGAS) |
| Repositorio: | RUNA. Repositorio da Consellería de Sanidade e Sergas |
| OAI Identifier: | oai:runa.sergas.gal:20.500.11940/21165 |
| Acceso en línea: | https://portalcientifico.sergas.gal//documentos/64be32f43bbfc602eae5916c http://hdl.handle.net/20.500.11940/21165 |
| Access Level: | acceso abierto |
| Palabra clave: | Humans Multiple Sclerosis Proteomics Brain Extracellular Vesicles Immune System Extracellular Matrix Biomarkers AS Santiago IDIS |
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Brain and immune system-derived extracellular vesicles mediate regulation of complement system, extracellular matrix remodeling, brain repair and antigen tolerance in Multiple sclerosisTorres Iglesias, G.Fernández-Fournier, M.Botella, L.Piniella, D.Laso-García, F.Carmen Gómez-de Frutos, M.Chamorro, B.Puertas, I.Tallón Barranco, A.Fuentes, B.Alonso de Leciñana, M.Alonso-López, E.Bravo López, Susana BelénEugenia Miranda-Carús, M.Montero-Calle, A.Barderas, R.Díez-Tejedor, E.Gutiérrez-Fernández, M.Otero-Ortega, L.HumansMultiple SclerosisProteomicsBrainExtracellular VesiclesImmune SystemExtracellular MatrixBiomarkersAS SantiagoIDISBackground: Multiple sclerosis (MS) is an immune-mediated central nervous system disease whose course is unpredictable. Finding biomarkers that help to better comprehend the disease's pathogenesis is crucial for supporting clinical decision-making. Blood extracellular vesicles (EVs) are membrane-bound particles secreted by all cell types that contain information on the disease's pathological processes. Purpose: To identify the immune and nervous system-derived EV profile from blood that could have a specific role as biomarker in MS and assess its possible correlation with disease state. Results: Higher levels of T cell-derived EVs and smaller size of neuron-derived EVs were associated with clinical relapse. The smaller size of the oligodendrocyte-derived EVs was related with motor and cognitive impairment. The proteomic analysis identified mannose-binding lectin serine protease 1 and complement factor H from immune system cell-derived EVs as autoimmune disease-associated proteins. We observed hepatocyte growth factor-like protein in EVs from T cells and inter-alpha-trypsin inhibitor heavy chain 2 from neurons as white matter injury-related proteins. In patients with MS, a specific protein profile was found in the EVs, higher levels of alpha-1-microglobulin and fibrinogen ? chain, lower levels of C1S and gelsolin in the immune system-released vesicles, and Talin-1 overexpression in oligodendrocyte EVs. These specific MS-associated proteins, as well as myelin basic protein in oligodendrocyte EVs, correlated with disease activity in the patients with MS. Conclusion: Neural-derived and immune-derived EVs found in blood appear to be good specific biomarkers in MS for reflecting the disease state.We greatly appreciate the support of Morote Traducciones S.L. for their editing assistance. This work was sponsored by a grant from Miguel Servet (CP20/00024 to Laura Otero-Ortega) , Miguel Servet (CPII20/00002 to Maria Gutie'rrez-Fern'andez) , a predoctoral fellowship (FI18/00026 to Fernando Laso-Garcia) , a Rio-Hortega grant (CM22/00065 to Gabriel Torres Iglesias and CM20/00047 to Elisa Alonso-L'opez) and by Research Project (PI21/00918) from the Instituto de Salud Carlos III and co-funded by the European Union and by a grant CA1/RSUE/2021-00753 to Dolores Piniella funded by Ministerio de Universidades, Plan de Recuperaci'on, Transformaci'on y Resiliencia y la Universidad Aut'onoma de Madrid.2023info:eu-repo/semantics/articlehttps://portalcientifico.sergas.gal//documentos/64be32f43bbfc602eae5916chttp://hdl.handle.net/20.500.11940/21165reponame:RUNA. Repositorio da Consellería de Sanidade e Sergasinstname:Servizo Galego de Saúde (SERGAS)Ingléshttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:runa.sergas.gal:20.500.11940/211652026-06-12T08:40:47Z |
| dc.title.none.fl_str_mv |
Brain and immune system-derived extracellular vesicles mediate regulation of complement system, extracellular matrix remodeling, brain repair and antigen tolerance in Multiple sclerosis |
| title |
Brain and immune system-derived extracellular vesicles mediate regulation of complement system, extracellular matrix remodeling, brain repair and antigen tolerance in Multiple sclerosis |
| spellingShingle |
Brain and immune system-derived extracellular vesicles mediate regulation of complement system, extracellular matrix remodeling, brain repair and antigen tolerance in Multiple sclerosis Torres Iglesias, G. Humans Multiple Sclerosis Proteomics Brain Extracellular Vesicles Immune System Extracellular Matrix Biomarkers AS Santiago IDIS |
| title_short |
Brain and immune system-derived extracellular vesicles mediate regulation of complement system, extracellular matrix remodeling, brain repair and antigen tolerance in Multiple sclerosis |
| title_full |
Brain and immune system-derived extracellular vesicles mediate regulation of complement system, extracellular matrix remodeling, brain repair and antigen tolerance in Multiple sclerosis |
| title_fullStr |
Brain and immune system-derived extracellular vesicles mediate regulation of complement system, extracellular matrix remodeling, brain repair and antigen tolerance in Multiple sclerosis |
| title_full_unstemmed |
Brain and immune system-derived extracellular vesicles mediate regulation of complement system, extracellular matrix remodeling, brain repair and antigen tolerance in Multiple sclerosis |
| title_sort |
Brain and immune system-derived extracellular vesicles mediate regulation of complement system, extracellular matrix remodeling, brain repair and antigen tolerance in Multiple sclerosis |
| dc.creator.none.fl_str_mv |
Torres Iglesias, G. Fernández-Fournier, M. Botella, L. Piniella, D. Laso-García, F. Carmen Gómez-de Frutos, M. Chamorro, B. Puertas, I. Tallón Barranco, A. Fuentes, B. Alonso de Leciñana, M. Alonso-López, E. Bravo López, Susana Belén Eugenia Miranda-Carús, M. Montero-Calle, A. Barderas, R. Díez-Tejedor, E. Gutiérrez-Fernández, M. Otero-Ortega, L. |
| author |
Torres Iglesias, G. |
| author_facet |
Torres Iglesias, G. Fernández-Fournier, M. Botella, L. Piniella, D. Laso-García, F. Carmen Gómez-de Frutos, M. Chamorro, B. Puertas, I. Tallón Barranco, A. Fuentes, B. Alonso de Leciñana, M. Alonso-López, E. Bravo López, Susana Belén Eugenia Miranda-Carús, M. Montero-Calle, A. Barderas, R. Díez-Tejedor, E. Gutiérrez-Fernández, M. Otero-Ortega, L. |
| author_role |
author |
| author2 |
Fernández-Fournier, M. Botella, L. Piniella, D. Laso-García, F. Carmen Gómez-de Frutos, M. Chamorro, B. Puertas, I. Tallón Barranco, A. Fuentes, B. Alonso de Leciñana, M. Alonso-López, E. Bravo López, Susana Belén Eugenia Miranda-Carús, M. Montero-Calle, A. Barderas, R. Díez-Tejedor, E. Gutiérrez-Fernández, M. Otero-Ortega, L. |
| author2_role |
author author author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Humans Multiple Sclerosis Proteomics Brain Extracellular Vesicles Immune System Extracellular Matrix Biomarkers AS Santiago IDIS |
| topic |
Humans Multiple Sclerosis Proteomics Brain Extracellular Vesicles Immune System Extracellular Matrix Biomarkers AS Santiago IDIS |
| description |
Background: Multiple sclerosis (MS) is an immune-mediated central nervous system disease whose course is unpredictable. Finding biomarkers that help to better comprehend the disease's pathogenesis is crucial for supporting clinical decision-making. Blood extracellular vesicles (EVs) are membrane-bound particles secreted by all cell types that contain information on the disease's pathological processes. Purpose: To identify the immune and nervous system-derived EV profile from blood that could have a specific role as biomarker in MS and assess its possible correlation with disease state. Results: Higher levels of T cell-derived EVs and smaller size of neuron-derived EVs were associated with clinical relapse. The smaller size of the oligodendrocyte-derived EVs was related with motor and cognitive impairment. The proteomic analysis identified mannose-binding lectin serine protease 1 and complement factor H from immune system cell-derived EVs as autoimmune disease-associated proteins. We observed hepatocyte growth factor-like protein in EVs from T cells and inter-alpha-trypsin inhibitor heavy chain 2 from neurons as white matter injury-related proteins. In patients with MS, a specific protein profile was found in the EVs, higher levels of alpha-1-microglobulin and fibrinogen ? chain, lower levels of C1S and gelsolin in the immune system-released vesicles, and Talin-1 overexpression in oligodendrocyte EVs. These specific MS-associated proteins, as well as myelin basic protein in oligodendrocyte EVs, correlated with disease activity in the patients with MS. Conclusion: Neural-derived and immune-derived EVs found in blood appear to be good specific biomarkers in MS for reflecting the disease state. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://portalcientifico.sergas.gal//documentos/64be32f43bbfc602eae5916c http://hdl.handle.net/20.500.11940/21165 |
| url |
https://portalcientifico.sergas.gal//documentos/64be32f43bbfc602eae5916c http://hdl.handle.net/20.500.11940/21165 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.rights.none.fl_str_mv |
http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by/4.0/ |
| eu_rights_str_mv |
openAccess |
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reponame:RUNA. Repositorio da Consellería de Sanidade e Sergas instname:Servizo Galego de Saúde (SERGAS) |
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Servizo Galego de Saúde (SERGAS) |
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RUNA. Repositorio da Consellería de Sanidade e Sergas |
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