Peptidoglycan recognition by wheat germ agglutinin. A view by NMR

10 p.-6 fig.-1 tab

Detalles Bibliográficos
Autores: El Biari, Khouzaima, Gaudioso, Ángel, Fernández-Alonso, M. Carmen, Jiménez-Barbero, Jesús, Cañada, F. Javier
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/183150
Acceso en línea:http://hdl.handle.net/10261/183150
Access Level:acceso abierto
Palabra clave:STD NMR
Anomer
Carbohydrates
WGA
Peptidoglycan interaction
Hevein domain
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spelling Peptidoglycan recognition by wheat germ agglutinin. A view by NMREl Biari, KhouzaimaGaudioso, ÁngelFernández-Alonso, M. CarmenJiménez-Barbero, JesúsCañada, F. JavierSTD NMRAnomerCarbohydratesWGAPeptidoglycan interactionHevein domain10 p.-6 fig.-1 tabWheat germ agglutinin (WGA) is a lectin composed of 4 homologous hevein domains. It has been shown that WGA binds N-acetyl glucosamine (GlcNAc)-related oligosaccharides and has applications as commercial reagent to detect glycans containing such modified residues. Peptidoglycan (PGN), the main component of the bacterial cell wall, is a polymeric material made of repeating disaccharide units of GlcNAc-N-acetylmuramic acid cross-linked with short polypeptide fragments. Wheat germ agglutinin is able to bind bacterial cells, a phenomenon that could correlate with its plant-defense capacities, but there is no information at the molecular level about how WGA binds to the PGN. Herein, we present structural data on the binding of a short PGN fragment to WGA by means of saturation transfer difference nuclear magnetic resonance studies. The results show that the GlcNAc residue establishes the major contacts with WGA, followed by the N-acetylmuramic acid residue. In contrast, the peptide moiety displays minor contacts at the binding site.We thank Agencia Estatal de Investigación of Spain (projects CTQ2015-64597-C2-1P, CTQ2015-64597-C2-2P, and SEV-2016-0644 Severo Ochoa Excellence Acreditation)Peer reviewedSage PublicationsMinisterio de Economía y Competitividad (España)Ministerio de Economía y Competitividad (España)Jiménez-Barbero, Jesús [0000-0001-5421-8513]Cañada, F. Javier [0000-0003-4462-1469]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/183150reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2015-64597-C2-1-Pinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2015-64597-C2-2-Pinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SEV-2016-0644https://doi.org/10.1177/1934578X19849240Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1831502026-05-22T06:33:51Z
dc.title.none.fl_str_mv Peptidoglycan recognition by wheat germ agglutinin. A view by NMR
title Peptidoglycan recognition by wheat germ agglutinin. A view by NMR
spellingShingle Peptidoglycan recognition by wheat germ agglutinin. A view by NMR
El Biari, Khouzaima
STD NMR
Anomer
Carbohydrates
WGA
Peptidoglycan interaction
Hevein domain
title_short Peptidoglycan recognition by wheat germ agglutinin. A view by NMR
title_full Peptidoglycan recognition by wheat germ agglutinin. A view by NMR
title_fullStr Peptidoglycan recognition by wheat germ agglutinin. A view by NMR
title_full_unstemmed Peptidoglycan recognition by wheat germ agglutinin. A view by NMR
title_sort Peptidoglycan recognition by wheat germ agglutinin. A view by NMR
dc.creator.none.fl_str_mv El Biari, Khouzaima
Gaudioso, Ángel
Fernández-Alonso, M. Carmen
Jiménez-Barbero, Jesús
Cañada, F. Javier
author El Biari, Khouzaima
author_facet El Biari, Khouzaima
Gaudioso, Ángel
Fernández-Alonso, M. Carmen
Jiménez-Barbero, Jesús
Cañada, F. Javier
author_role author
author2 Gaudioso, Ángel
Fernández-Alonso, M. Carmen
Jiménez-Barbero, Jesús
Cañada, F. Javier
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Ministerio de Economía y Competitividad (España)
Jiménez-Barbero, Jesús [0000-0001-5421-8513]
Cañada, F. Javier [0000-0003-4462-1469]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv STD NMR
Anomer
Carbohydrates
WGA
Peptidoglycan interaction
Hevein domain
topic STD NMR
Anomer
Carbohydrates
WGA
Peptidoglycan interaction
Hevein domain
description 10 p.-6 fig.-1 tab
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/183150
url http://hdl.handle.net/10261/183150
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2015-64597-C2-1-P
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2015-64597-C2-2-P
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SEV-2016-0644
https://doi.org/10.1177/1934578X19849240
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Sage Publications
publisher.none.fl_str_mv Sage Publications
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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