Exploring the Potential of Various Cyclodextrin-Based Derivatives in Enzyme Supramolecular Engineering

Articulo e información suplementaria (Supporting information)

Detalles Bibliográficos
Autores: Foroutan Kalourazi, Ali, Amirabbas Nazemi, Seyed, Unniram Parambil, Ajmal Roshan, Ferrer, Manuel, Shahangian, S. Shirin, Shahgaldian, Patrick
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/374627
Acceso en línea:http://hdl.handle.net/10261/374627
Access Level:acceso abierto
Palabra clave:Supramolecular enzyme engineering
Protein stability
Cyclodextrin
Immobilization
Shielding
http://metadata.un.org/sdg/12
Ensure sustainable consumption and production patterns
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spelling Exploring the Potential of Various Cyclodextrin-Based Derivatives in Enzyme Supramolecular EngineeringForoutan Kalourazi, AliAmirabbas Nazemi, SeyedUnniram Parambil, Ajmal RoshanFerrer, ManuelShahangian, S. ShirinShahgaldian, PatrickSupramolecular enzyme engineeringProtein stabilityCyclodextrinImmobilizationShieldinghttp://metadata.un.org/sdg/12Ensure sustainable consumption and production patternsArticulo e información suplementaria (Supporting information)Enzyme stability and activity are pivotal factors for their implementation in different industrial applications. Enzyme supramolecular engineering relies on the fabrication of a tailor-made enzyme nano-environment to ensure enzyme stability without impairing activity. Cyclodextrins (CDs), cyclic oligomers of glucose, act as protein chaperones and stabilize, upon interaction with hydrophobic amino acid residues exposed at the protein surface, its three-dimensional structure. When used to build an organosilica layer shielding an enzyme, they enhance the protective effect of this layer. In the present study, we systematically assessed the protective effects of three organosilane derivatives based on alpha-, β- and γ-CDs. A model lipase enzyme was immobilized at the surface of silica nano- particles and shielded in an organosilica layer containing these organosilanes. Besides layer thickness optimization, the effect of different stressors (i. e., temperature, SDS, urea) was tested. Our results showed that organosilica layers produced with CDs improve enzyme thermal stability. They also support enzyme refolding after denaturation under chaotic conditions. Additionally, we demonstrated that the protective effect of the smallest CD derivative tested, namely alpha-CD, surpassed the other macrocycles studied for conferring the immobilized enzyme with higher resistance to stress conditions. This protection strategy was also applied to a thermostable β-galactosidase enzyme.I. Materials and Method; II. LipMRD9 purification and quantification; III. CD-TES characterisation; IV. SEM micrographs; V. ReferencesPeer reviewedJohn Wiley & SonsEuropean CommissionMinisterio de Ciencia, Innovación y Universidades (España)Foroutan Kalourazi, Ali [0000-0002-8176-3928]Amirabbas Nazemi, Seyed [0009-0002-9531-5365]Unniram Parambil, Ajmal Roshan [0000-0002-4244-537X]Ferrer, Manuel [0000-0003-4962-4714]Shahangian, S. Shirin [0000-0001-9861-4702]Shahgaldian, Patrick [0000-0002-1743-0387]Foroutan Kalourazi, AliAmirabbas Nazemi, SeyedUnniram Parambil, Ajmal RoshanFerrer, ManuelShahangian, S. ShirinShahgaldian, PatrickConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_dcae04bcPublisher's versioninfo:eu-repo/semantics/publishedVersionpdfhttp://hdl.handle.net/10261/374627reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#101000327PID2020-112758RBPDC2021-121534-I00TED2021-130544Bhttps://doi.org/10.1002/cbic.202400840Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3746272026-05-22T06:33:51Z
dc.title.none.fl_str_mv Exploring the Potential of Various Cyclodextrin-Based Derivatives in Enzyme Supramolecular Engineering
title Exploring the Potential of Various Cyclodextrin-Based Derivatives in Enzyme Supramolecular Engineering
spellingShingle Exploring the Potential of Various Cyclodextrin-Based Derivatives in Enzyme Supramolecular Engineering
Foroutan Kalourazi, Ali
Supramolecular enzyme engineering
Protein stability
Cyclodextrin
Immobilization
Shielding
http://metadata.un.org/sdg/12
Ensure sustainable consumption and production patterns
title_short Exploring the Potential of Various Cyclodextrin-Based Derivatives in Enzyme Supramolecular Engineering
title_full Exploring the Potential of Various Cyclodextrin-Based Derivatives in Enzyme Supramolecular Engineering
title_fullStr Exploring the Potential of Various Cyclodextrin-Based Derivatives in Enzyme Supramolecular Engineering
title_full_unstemmed Exploring the Potential of Various Cyclodextrin-Based Derivatives in Enzyme Supramolecular Engineering
title_sort Exploring the Potential of Various Cyclodextrin-Based Derivatives in Enzyme Supramolecular Engineering
dc.creator.none.fl_str_mv Foroutan Kalourazi, Ali
Amirabbas Nazemi, Seyed
Unniram Parambil, Ajmal Roshan
Ferrer, Manuel
Shahangian, S. Shirin
Shahgaldian, Patrick
author Foroutan Kalourazi, Ali
author_facet Foroutan Kalourazi, Ali
Amirabbas Nazemi, Seyed
Unniram Parambil, Ajmal Roshan
Ferrer, Manuel
Shahangian, S. Shirin
Shahgaldian, Patrick
author_role author
author2 Amirabbas Nazemi, Seyed
Unniram Parambil, Ajmal Roshan
Ferrer, Manuel
Shahangian, S. Shirin
Shahgaldian, Patrick
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Ministerio de Ciencia, Innovación y Universidades (España)
Foroutan Kalourazi, Ali [0000-0002-8176-3928]
Amirabbas Nazemi, Seyed [0009-0002-9531-5365]
Unniram Parambil, Ajmal Roshan [0000-0002-4244-537X]
Ferrer, Manuel [0000-0003-4962-4714]
Shahangian, S. Shirin [0000-0001-9861-4702]
Shahgaldian, Patrick [0000-0002-1743-0387]
Foroutan Kalourazi, Ali
Amirabbas Nazemi, Seyed
Unniram Parambil, Ajmal Roshan
Ferrer, Manuel
Shahangian, S. Shirin
Shahgaldian, Patrick
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Supramolecular enzyme engineering
Protein stability
Cyclodextrin
Immobilization
Shielding
http://metadata.un.org/sdg/12
Ensure sustainable consumption and production patterns
topic Supramolecular enzyme engineering
Protein stability
Cyclodextrin
Immobilization
Shielding
http://metadata.un.org/sdg/12
Ensure sustainable consumption and production patterns
description Articulo e información suplementaria (Supporting information)
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_dcae04bc
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/374627
url http://hdl.handle.net/10261/374627
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
101000327
PID2020-112758RB
PDC2021-121534-I00
TED2021-130544B
https://doi.org/10.1002/cbic.202400840
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv pdf
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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