Identification of an atypical replicative genetic element in Rhodococcus jostii RHA1

8 p.-3 fig.

Detalhes bibliográficos
Autores: Acedos, Miguel G., De la Torre, Isabel, Barriuso, Jorge, García, José Luis
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/393031
Acesso em linha:http://hdl.handle.net/10261/393031
Access Level:acceso abierto
Palavra-chave:Rhodococcus
Kanamycin
Illegitimate recombination
Replicative genetic element
Antibiotic resistance
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spelling Identification of an atypical replicative genetic element in Rhodococcus jostii RHA1Acedos, Miguel G.De la Torre, IsabelBarriuso, JorgeGarcía, José LuisRhodococcusKanamycinIllegitimate recombinationReplicative genetic elementAntibiotic resistance8 p.-3 fig.By exploring the use of plasmids to confer Rhodococcus jostii RHA1 the possibility of utilizing xylose to produce lipids we have observed that the plasmid used was not always maintained in the transformants as expected. Instead, we observed an illegitimate integration of the antibiotic resistance gene from the plasmid into the recombinant cells. Genome sequencing of the transformants has provided evidence that this illegitimate integration is not size-, site-or sequence-specific. But even more surprising, genome sequencing revealed the presence of an unexpected circular multicopy replicative element (75–80 kb) that appears to be excised from the chromosome as a consequence of the stress generated by the antibiotic used in the selection process. The excised fragment does not contain any of the typical features of genomic islands. These results provide evidence that the genome of this oleaginous strain is more plastic than initially anticipated and our findings open the option of developing new ways to genetically modify this strain by using illegitimate recombinant approaches. But even more remarkably, the discovery of this atypical replicative element raises new questions about the existence of novel mechanisms of evolution in bacteria.This work has been carried out in the framework of the European project FRONTSH1P, which has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement no. 101037031.Peer reviewedFrontiers MediaEuropean CommissionAcedos, Miguel G. [0000-0003-4229-5698]De la Torre, Isabel [0000-0002-0460-8027]Barriuso, Jorge [0000-0003-0916-6560]García, José Luis [0000-0002-9238-2485]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/393031reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/H2020/101037031https://doi.org/10.3389/fmicb.2025.1567901Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3930312026-05-22T06:33:51Z
dc.title.none.fl_str_mv Identification of an atypical replicative genetic element in Rhodococcus jostii RHA1
title Identification of an atypical replicative genetic element in Rhodococcus jostii RHA1
spellingShingle Identification of an atypical replicative genetic element in Rhodococcus jostii RHA1
Acedos, Miguel G.
Rhodococcus
Kanamycin
Illegitimate recombination
Replicative genetic element
Antibiotic resistance
title_short Identification of an atypical replicative genetic element in Rhodococcus jostii RHA1
title_full Identification of an atypical replicative genetic element in Rhodococcus jostii RHA1
title_fullStr Identification of an atypical replicative genetic element in Rhodococcus jostii RHA1
title_full_unstemmed Identification of an atypical replicative genetic element in Rhodococcus jostii RHA1
title_sort Identification of an atypical replicative genetic element in Rhodococcus jostii RHA1
dc.creator.none.fl_str_mv Acedos, Miguel G.
De la Torre, Isabel
Barriuso, Jorge
García, José Luis
author Acedos, Miguel G.
author_facet Acedos, Miguel G.
De la Torre, Isabel
Barriuso, Jorge
García, José Luis
author_role author
author2 De la Torre, Isabel
Barriuso, Jorge
García, José Luis
author2_role author
author
author
dc.contributor.none.fl_str_mv European Commission
Acedos, Miguel G. [0000-0003-4229-5698]
De la Torre, Isabel [0000-0002-0460-8027]
Barriuso, Jorge [0000-0003-0916-6560]
García, José Luis [0000-0002-9238-2485]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Rhodococcus
Kanamycin
Illegitimate recombination
Replicative genetic element
Antibiotic resistance
topic Rhodococcus
Kanamycin
Illegitimate recombination
Replicative genetic element
Antibiotic resistance
description 8 p.-3 fig.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/393031
url http://hdl.handle.net/10261/393031
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/H2020/101037031
https://doi.org/10.3389/fmicb.2025.1567901
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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