Hypothalamic endocannabinoids inversely correlate with the development of diet-induced obesity in male and female mice

The endocannabinoid (eCB) system regulates energy homeostasis and is linked to obesity development. However, the exact dynamic and regulation of eCBs in the hypothalamus during obesity progression remain incompletely described and understood. Our study examined the time course of responses in two hy...

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Autores: Miralpeix, Cristina, Fosch, Anna, Casas Brugulat, Josefina, Baena Muñoz, Miguel, Herrero Rodríguez, Laura, Serra i Cucurull, Dolors, Rodríguez-Rodríguez, Rosalía, Casals i Farré, Núria
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/143720
Acceso en línea:https://hdl.handle.net/2445/143720
Access Level:acceso abierto
Palabra clave:Hipotàlem
Dimorfisme sexual en els animals
Teixit adipós
Obesitat
Hypothalamus
Sexual dimorphism (Animals)
Adipose tissues
Obesity
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spelling Hypothalamic endocannabinoids inversely correlate with the development of diet-induced obesity in male and female miceMiralpeix, CristinaFosch, AnnaCasas Brugulat, JosefinaBaena Muñoz, MiguelHerrero Rodríguez, LauraSerra i Cucurull, DolorsRodríguez-Rodríguez, RosalíaCasals i Farré, NúriaHipotàlemDimorfisme sexual en els animalsTeixit adipósObesitatHypothalamusSexual dimorphism (Animals)Adipose tissuesObesityThe endocannabinoid (eCB) system regulates energy homeostasis and is linked to obesity development. However, the exact dynamic and regulation of eCBs in the hypothalamus during obesity progression remain incompletely described and understood. Our study examined the time course of responses in two hypothalamic eCBs, 2-arachidonoylglycerol (2-AG) and arachidonoylethanolamine (AEA), in male and female mice during diet-induced obesity and explored the association of eCB levels with changes in brown adipose tissue (BAT) thermogenesis and body weight. We fed mice a high-fat diet (HFD), which induced a transient increase (substantial at 7 days) in hypothalamic eCBs, followed by a progressive decrease to basal levels with a long-term HFD. This transient rise at early stages of obesity is considered a physiologic compensatory response to BAT thermogenesis, which is activated by diet surplus. The eCB dynamic was sexually dimorphic: hypothalamic eCBs levels were higher in female mice, who became obese at later time points than males. The hypothalamic eCBs time course positively correlated with thermogenesis activation, but negatively matched body weight, leptinemia, and circulating eCB levels. Increased expression of eCB-synthetizing enzymes accompanied the transient hypothalamic eCB elevation. Icv injection of eCB did not promote BAT thermogenesis; however, administration of thermogenic molecules, such as central leptin or a peripheral β3-adrenoreceptor agonist, induced a significant increase in hypothalamic eCBs, suggesting a directional link from BAT thermogenesis to hypothalamic eCBs. This study contributes to the understanding of hypothalamic regulation of obesity. Keywords: hypothalamus, sexual dimorphism, brown adipose tissueAmerican Society for Biochemistry and Molecular Biology2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/143720Articles publicats en revistes (Bioquímica i Fisiologia)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1194/jlr.M092742Journal of Lipid Research, 2019, vol. 60, p. 1260-1269https://doi.org/10.1194/jlr.M092742(c) Miralpeix, Cristina et al., 2019info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1437202026-05-27T06:46:51Z
dc.title.none.fl_str_mv Hypothalamic endocannabinoids inversely correlate with the development of diet-induced obesity in male and female mice
title Hypothalamic endocannabinoids inversely correlate with the development of diet-induced obesity in male and female mice
spellingShingle Hypothalamic endocannabinoids inversely correlate with the development of diet-induced obesity in male and female mice
Miralpeix, Cristina
Hipotàlem
Dimorfisme sexual en els animals
Teixit adipós
Obesitat
Hypothalamus
Sexual dimorphism (Animals)
Adipose tissues
Obesity
title_short Hypothalamic endocannabinoids inversely correlate with the development of diet-induced obesity in male and female mice
title_full Hypothalamic endocannabinoids inversely correlate with the development of diet-induced obesity in male and female mice
title_fullStr Hypothalamic endocannabinoids inversely correlate with the development of diet-induced obesity in male and female mice
title_full_unstemmed Hypothalamic endocannabinoids inversely correlate with the development of diet-induced obesity in male and female mice
title_sort Hypothalamic endocannabinoids inversely correlate with the development of diet-induced obesity in male and female mice
dc.creator.none.fl_str_mv Miralpeix, Cristina
Fosch, Anna
Casas Brugulat, Josefina
Baena Muñoz, Miguel
Herrero Rodríguez, Laura
Serra i Cucurull, Dolors
Rodríguez-Rodríguez, Rosalía
Casals i Farré, Núria
author Miralpeix, Cristina
author_facet Miralpeix, Cristina
Fosch, Anna
Casas Brugulat, Josefina
Baena Muñoz, Miguel
Herrero Rodríguez, Laura
Serra i Cucurull, Dolors
Rodríguez-Rodríguez, Rosalía
Casals i Farré, Núria
author_role author
author2 Fosch, Anna
Casas Brugulat, Josefina
Baena Muñoz, Miguel
Herrero Rodríguez, Laura
Serra i Cucurull, Dolors
Rodríguez-Rodríguez, Rosalía
Casals i Farré, Núria
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Hipotàlem
Dimorfisme sexual en els animals
Teixit adipós
Obesitat
Hypothalamus
Sexual dimorphism (Animals)
Adipose tissues
Obesity
topic Hipotàlem
Dimorfisme sexual en els animals
Teixit adipós
Obesitat
Hypothalamus
Sexual dimorphism (Animals)
Adipose tissues
Obesity
description The endocannabinoid (eCB) system regulates energy homeostasis and is linked to obesity development. However, the exact dynamic and regulation of eCBs in the hypothalamus during obesity progression remain incompletely described and understood. Our study examined the time course of responses in two hypothalamic eCBs, 2-arachidonoylglycerol (2-AG) and arachidonoylethanolamine (AEA), in male and female mice during diet-induced obesity and explored the association of eCB levels with changes in brown adipose tissue (BAT) thermogenesis and body weight. We fed mice a high-fat diet (HFD), which induced a transient increase (substantial at 7 days) in hypothalamic eCBs, followed by a progressive decrease to basal levels with a long-term HFD. This transient rise at early stages of obesity is considered a physiologic compensatory response to BAT thermogenesis, which is activated by diet surplus. The eCB dynamic was sexually dimorphic: hypothalamic eCBs levels were higher in female mice, who became obese at later time points than males. The hypothalamic eCBs time course positively correlated with thermogenesis activation, but negatively matched body weight, leptinemia, and circulating eCB levels. Increased expression of eCB-synthetizing enzymes accompanied the transient hypothalamic eCB elevation. Icv injection of eCB did not promote BAT thermogenesis; however, administration of thermogenic molecules, such as central leptin or a peripheral β3-adrenoreceptor agonist, induced a significant increase in hypothalamic eCBs, suggesting a directional link from BAT thermogenesis to hypothalamic eCBs. This study contributes to the understanding of hypothalamic regulation of obesity. Keywords: hypothalamus, sexual dimorphism, brown adipose tissue
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/143720
url https://hdl.handle.net/2445/143720
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1194/jlr.M092742
Journal of Lipid Research, 2019, vol. 60, p. 1260-1269
https://doi.org/10.1194/jlr.M092742
dc.rights.none.fl_str_mv (c) Miralpeix, Cristina et al., 2019
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Miralpeix, Cristina et al., 2019
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Fisiologia)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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