Angiotensin II Promotes Skeletal Muscle Angiogenesis Induced by Volume-Dependent Aerobic Exercise Training: Effects on miRNAs-27a/b and Oxidant–Antioxidant Balance

Aerobic exercise training (ET) produces beneficial adaptations in skeletal muscles, including angiogenesis. The renin–angiotensin system (RAS) is highly involved in angiogenesis stimuli. However, the molecular mechanisms underlying capillary growth in skeletal muscle induced by aerobic ET are not co...

Descripción completa

Detalles Bibliográficos
Autores: Rodrigues, Luis Felipe, Pelozin, Bruno Rocha Avila, da Silva Junior, Natan Daniel, Soci, Ursula Paula Renó, Crovoi do Carmo, Everton, Alves da Mota, Glória de Fatima, Cachofeiro Ramos, María Victoria, Lahera Julia, Vicente, Oliveira, Edilamar Menezes, Fernandes, Tiago
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/71749
Acceso en línea:https://hdl.handle.net/20.500.14352/71749
Access Level:acceso abierto
Palabra clave:Aerobic training
MicroRNAs
Angiogenesis
Renin–angiotensin system
Redox balance
Medicina del deporte
Medicina Física y Rehabilitación
Sistema musculoesquelético
3204.04 Rehabilitación (Medica)
2411.10 Fisiología del Músculo
id ES_ce7d99a4eea2cf4e78a25bb3e99934b5
oai_identifier_str oai:docta.ucm.es:20.500.14352/71749
network_acronym_str ES
network_name_str España
repository_id_str
spelling Angiotensin II Promotes Skeletal Muscle Angiogenesis Induced by Volume-Dependent Aerobic Exercise Training: Effects on miRNAs-27a/b and Oxidant–Antioxidant BalanceRodrigues, Luis FelipePelozin, Bruno Rocha Avilada Silva Junior, Natan DanielSoci, Ursula Paula RenóCrovoi do Carmo, EvertonAlves da Mota, Glória de FatimaCachofeiro Ramos, María VictoriaLahera Julia, VicenteOliveira, Edilamar MenezesFernandes, TiagoAerobic trainingMicroRNAsAngiogenesisRenin–angiotensin systemRedox balanceMedicina del deporteMedicina Física y RehabilitaciónSistema musculoesquelético3204.04 Rehabilitación (Medica)2411.10 Fisiología del MúsculoAerobic exercise training (ET) produces beneficial adaptations in skeletal muscles, including angiogenesis. The renin–angiotensin system (RAS) is highly involved in angiogenesis stimuli. However, the molecular mechanisms underlying capillary growth in skeletal muscle induced by aerobic ET are not completely understood. This study aimed to investigate the effects of volume-dependent aerobic ET on skeletal muscle angiogenesis involving the expression of miRNAs-27a and 27b on RAS and oxidant–antioxidant balance. Eight-week-old female Wistar rats were divided into three groups: sedentary control (SC), trained protocol 1 (P1), and trained protocol 2 (P2). P1 consisted of 60 min/day of swimming, 5×/week, for 10 weeks. P2 consisted of the same protocol as P1 until the 8th week, but in the 9th week, rats trained 2×/day, and in the 10th week, trained 3×/day. Angiogenesis and molecular analyses were performed in soleus muscle samples. Furthermore, to establish ET-induced angiogenesis through RAS, animals were treated with an AT1 receptor blocker (losartan). Aerobic ET promoted higher VO2 peak and exercise tolerance values. In contrast, miRNA-27a and -27b levels were reduced in both trained groups, compared with the SC group. This was in parallel with an increase in the ACE1/Ang II/VEGF axis, which led to a higher capillary-to-fiber ratio. Moreover, aerobic ET induced an antioxidant profile increasing skeletal muscle SOD2 and catalase gene expression, which was accompanied by high nitrite levels and reduced nitrotyrosine concentrations in the circulation. Additionally, losartan treatment partially re-established the miRNAs expression and the capillary-to-fiber ratio in the trained groups. In summary, aerobic ET promoted angiogenesis through the miRNA-27a/b–ACE1/Ang II/VEGF axis and improved the redox balance. Losartan treatment demonstrates the participation of RAS in ET-induced vascular growth. miRNAs and RAS components are promising potential targets to modulate angiogenesis for combating vascular diseases, as well as potential biomarkers to monitor training interventions and physical performance.MPDIUniversidad Complutense de Madrid20222022-03-2820222022-03-28journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/71749reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/717492026-06-02T12:44:21Z
dc.title.none.fl_str_mv Angiotensin II Promotes Skeletal Muscle Angiogenesis Induced by Volume-Dependent Aerobic Exercise Training: Effects on miRNAs-27a/b and Oxidant–Antioxidant Balance
title Angiotensin II Promotes Skeletal Muscle Angiogenesis Induced by Volume-Dependent Aerobic Exercise Training: Effects on miRNAs-27a/b and Oxidant–Antioxidant Balance
spellingShingle Angiotensin II Promotes Skeletal Muscle Angiogenesis Induced by Volume-Dependent Aerobic Exercise Training: Effects on miRNAs-27a/b and Oxidant–Antioxidant Balance
Rodrigues, Luis Felipe
Aerobic training
MicroRNAs
Angiogenesis
Renin–angiotensin system
Redox balance
Medicina del deporte
Medicina Física y Rehabilitación
Sistema musculoesquelético
3204.04 Rehabilitación (Medica)
2411.10 Fisiología del Músculo
title_short Angiotensin II Promotes Skeletal Muscle Angiogenesis Induced by Volume-Dependent Aerobic Exercise Training: Effects on miRNAs-27a/b and Oxidant–Antioxidant Balance
title_full Angiotensin II Promotes Skeletal Muscle Angiogenesis Induced by Volume-Dependent Aerobic Exercise Training: Effects on miRNAs-27a/b and Oxidant–Antioxidant Balance
title_fullStr Angiotensin II Promotes Skeletal Muscle Angiogenesis Induced by Volume-Dependent Aerobic Exercise Training: Effects on miRNAs-27a/b and Oxidant–Antioxidant Balance
title_full_unstemmed Angiotensin II Promotes Skeletal Muscle Angiogenesis Induced by Volume-Dependent Aerobic Exercise Training: Effects on miRNAs-27a/b and Oxidant–Antioxidant Balance
title_sort Angiotensin II Promotes Skeletal Muscle Angiogenesis Induced by Volume-Dependent Aerobic Exercise Training: Effects on miRNAs-27a/b and Oxidant–Antioxidant Balance
dc.creator.none.fl_str_mv Rodrigues, Luis Felipe
Pelozin, Bruno Rocha Avila
da Silva Junior, Natan Daniel
Soci, Ursula Paula Renó
Crovoi do Carmo, Everton
Alves da Mota, Glória de Fatima
Cachofeiro Ramos, María Victoria
Lahera Julia, Vicente
Oliveira, Edilamar Menezes
Fernandes, Tiago
author Rodrigues, Luis Felipe
author_facet Rodrigues, Luis Felipe
Pelozin, Bruno Rocha Avila
da Silva Junior, Natan Daniel
Soci, Ursula Paula Renó
Crovoi do Carmo, Everton
Alves da Mota, Glória de Fatima
Cachofeiro Ramos, María Victoria
Lahera Julia, Vicente
Oliveira, Edilamar Menezes
Fernandes, Tiago
author_role author
author2 Pelozin, Bruno Rocha Avila
da Silva Junior, Natan Daniel
Soci, Ursula Paula Renó
Crovoi do Carmo, Everton
Alves da Mota, Glória de Fatima
Cachofeiro Ramos, María Victoria
Lahera Julia, Vicente
Oliveira, Edilamar Menezes
Fernandes, Tiago
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv Aerobic training
MicroRNAs
Angiogenesis
Renin–angiotensin system
Redox balance
Medicina del deporte
Medicina Física y Rehabilitación
Sistema musculoesquelético
3204.04 Rehabilitación (Medica)
2411.10 Fisiología del Músculo
topic Aerobic training
MicroRNAs
Angiogenesis
Renin–angiotensin system
Redox balance
Medicina del deporte
Medicina Física y Rehabilitación
Sistema musculoesquelético
3204.04 Rehabilitación (Medica)
2411.10 Fisiología del Músculo
description Aerobic exercise training (ET) produces beneficial adaptations in skeletal muscles, including angiogenesis. The renin–angiotensin system (RAS) is highly involved in angiogenesis stimuli. However, the molecular mechanisms underlying capillary growth in skeletal muscle induced by aerobic ET are not completely understood. This study aimed to investigate the effects of volume-dependent aerobic ET on skeletal muscle angiogenesis involving the expression of miRNAs-27a and 27b on RAS and oxidant–antioxidant balance. Eight-week-old female Wistar rats were divided into three groups: sedentary control (SC), trained protocol 1 (P1), and trained protocol 2 (P2). P1 consisted of 60 min/day of swimming, 5×/week, for 10 weeks. P2 consisted of the same protocol as P1 until the 8th week, but in the 9th week, rats trained 2×/day, and in the 10th week, trained 3×/day. Angiogenesis and molecular analyses were performed in soleus muscle samples. Furthermore, to establish ET-induced angiogenesis through RAS, animals were treated with an AT1 receptor blocker (losartan). Aerobic ET promoted higher VO2 peak and exercise tolerance values. In contrast, miRNA-27a and -27b levels were reduced in both trained groups, compared with the SC group. This was in parallel with an increase in the ACE1/Ang II/VEGF axis, which led to a higher capillary-to-fiber ratio. Moreover, aerobic ET induced an antioxidant profile increasing skeletal muscle SOD2 and catalase gene expression, which was accompanied by high nitrite levels and reduced nitrotyrosine concentrations in the circulation. Additionally, losartan treatment partially re-established the miRNAs expression and the capillary-to-fiber ratio in the trained groups. In summary, aerobic ET promoted angiogenesis through the miRNA-27a/b–ACE1/Ang II/VEGF axis and improved the redox balance. Losartan treatment demonstrates the participation of RAS in ET-induced vascular growth. miRNAs and RAS components are promising potential targets to modulate angiogenesis for combating vascular diseases, as well as potential biomarkers to monitor training interventions and physical performance.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-03-28
2022
2022-03-28
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/71749
url https://hdl.handle.net/20.500.14352/71749
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MPDI
publisher.none.fl_str_mv MPDI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869419995653472256
score 15,300719

Similares en LA Referencia