Human Oral Epithelial Cells Impair Bacteria-Mediated Maturation of Dendritic Cells and Render T Cells Unresponsive to Stimulation

The oral mucosa is a first line of defense against pathogenic organisms and yet tolerates food antigens and resident bacteria. Mucosal epithelial cells are emerging as important regulators of innate and adaptive immune responses. However, the contribution of oral epithelial cells (OECs) determining...

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Autores: Molero Abraham, Magdalena, Sánchez Trincado López, José Luis, Gómez Perosanz, Marta, Torres Gómez, Álvaro, Subiza Garrido-Lestache, José Luis, Lafuente Duarte, María Esther, Reche Gallardo, Pedro Antonio
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/116159
Acceso en línea:https://hdl.handle.net/20.500.14352/116159
Access Level:acceso abierto
Palabra clave:612.017
T cells
Bacteria
Dendritic cells
Immunomodulation
Oral epithelial cells
Ciencias Biomédicas
Inmunología
24 Ciencias de la Vida
2412 Inmunología
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oai_identifier_str oai:docta.ucm.es:20.500.14352/116159
network_acronym_str ES
network_name_str España
repository_id_str
spelling Human Oral Epithelial Cells Impair Bacteria-Mediated Maturation of Dendritic Cells and Render T Cells Unresponsive to StimulationMolero Abraham, MagdalenaSánchez Trincado López, José LuisGómez Perosanz, MartaTorres Gómez, ÁlvaroSubiza Garrido-Lestache, José LuisLafuente Duarte, María EstherReche Gallardo, Pedro Antonio612.017T cellsBacteriaDendritic cellsImmunomodulationOral epithelial cellsCiencias BiomédicasInmunología24 Ciencias de la Vida2412 InmunologíaThe oral mucosa is a first line of defense against pathogenic organisms and yet tolerates food antigens and resident bacteria. Mucosal epithelial cells are emerging as important regulators of innate and adaptive immune responses. However, the contribution of oral epithelial cells (OECs) determining oral immunity is understudied. Here, we evaluated the ability of H413 and TR146 cells, two OEC lines derived from human oral squamous cell carcinomas, and primary OECs to modulate immune responses to a cocktail of Gram+ and Gram- bacteria known as MV130. OECs expressed CD40 constitutively and class II major histocompatibility complex (MHC II) molecules when stimulated with IFNγ, but not CD80 or CD86. Dendritic cells (DCs) treated with bacteria in co-culture with OECs did not fully mature, as judged by the expression of MHC II, CD80 and CD86, and barely released IL-12 and TNFα, compared to control DCs. Furthermore, in the presence of OECs, DCs were unable to stimulate allogenic naive CD4 T cells to produce IFNγ and TNFα. Similarly, OECs in culture with total CD4 T cells or Th1 cells stimulated with anti-CD3 and anti-CD28 antibodies abrogated CD25 and CD69 expression, T cell proliferation and the release of IFNγ and TNFα. The inhibition on T cell activation by OECs was cell-contact dependent, TGFβ independent and largely irreversible. Overall, this behavior of OECs is likely key to avoid immune system over-reaction against resident bacteria.FrontiersUniversidad Complutense de Madrid20192019-06-2820192019-06-28journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/116159reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1161592026-06-02T12:44:21Z
dc.title.none.fl_str_mv Human Oral Epithelial Cells Impair Bacteria-Mediated Maturation of Dendritic Cells and Render T Cells Unresponsive to Stimulation
title Human Oral Epithelial Cells Impair Bacteria-Mediated Maturation of Dendritic Cells and Render T Cells Unresponsive to Stimulation
spellingShingle Human Oral Epithelial Cells Impair Bacteria-Mediated Maturation of Dendritic Cells and Render T Cells Unresponsive to Stimulation
Molero Abraham, Magdalena
612.017
T cells
Bacteria
Dendritic cells
Immunomodulation
Oral epithelial cells
Ciencias Biomédicas
Inmunología
24 Ciencias de la Vida
2412 Inmunología
title_short Human Oral Epithelial Cells Impair Bacteria-Mediated Maturation of Dendritic Cells and Render T Cells Unresponsive to Stimulation
title_full Human Oral Epithelial Cells Impair Bacteria-Mediated Maturation of Dendritic Cells and Render T Cells Unresponsive to Stimulation
title_fullStr Human Oral Epithelial Cells Impair Bacteria-Mediated Maturation of Dendritic Cells and Render T Cells Unresponsive to Stimulation
title_full_unstemmed Human Oral Epithelial Cells Impair Bacteria-Mediated Maturation of Dendritic Cells and Render T Cells Unresponsive to Stimulation
title_sort Human Oral Epithelial Cells Impair Bacteria-Mediated Maturation of Dendritic Cells and Render T Cells Unresponsive to Stimulation
dc.creator.none.fl_str_mv Molero Abraham, Magdalena
Sánchez Trincado López, José Luis
Gómez Perosanz, Marta
Torres Gómez, Álvaro
Subiza Garrido-Lestache, José Luis
Lafuente Duarte, María Esther
Reche Gallardo, Pedro Antonio
author Molero Abraham, Magdalena
author_facet Molero Abraham, Magdalena
Sánchez Trincado López, José Luis
Gómez Perosanz, Marta
Torres Gómez, Álvaro
Subiza Garrido-Lestache, José Luis
Lafuente Duarte, María Esther
Reche Gallardo, Pedro Antonio
author_role author
author2 Sánchez Trincado López, José Luis
Gómez Perosanz, Marta
Torres Gómez, Álvaro
Subiza Garrido-Lestache, José Luis
Lafuente Duarte, María Esther
Reche Gallardo, Pedro Antonio
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 612.017
T cells
Bacteria
Dendritic cells
Immunomodulation
Oral epithelial cells
Ciencias Biomédicas
Inmunología
24 Ciencias de la Vida
2412 Inmunología
topic 612.017
T cells
Bacteria
Dendritic cells
Immunomodulation
Oral epithelial cells
Ciencias Biomédicas
Inmunología
24 Ciencias de la Vida
2412 Inmunología
description The oral mucosa is a first line of defense against pathogenic organisms and yet tolerates food antigens and resident bacteria. Mucosal epithelial cells are emerging as important regulators of innate and adaptive immune responses. However, the contribution of oral epithelial cells (OECs) determining oral immunity is understudied. Here, we evaluated the ability of H413 and TR146 cells, two OEC lines derived from human oral squamous cell carcinomas, and primary OECs to modulate immune responses to a cocktail of Gram+ and Gram- bacteria known as MV130. OECs expressed CD40 constitutively and class II major histocompatibility complex (MHC II) molecules when stimulated with IFNγ, but not CD80 or CD86. Dendritic cells (DCs) treated with bacteria in co-culture with OECs did not fully mature, as judged by the expression of MHC II, CD80 and CD86, and barely released IL-12 and TNFα, compared to control DCs. Furthermore, in the presence of OECs, DCs were unable to stimulate allogenic naive CD4 T cells to produce IFNγ and TNFα. Similarly, OECs in culture with total CD4 T cells or Th1 cells stimulated with anti-CD3 and anti-CD28 antibodies abrogated CD25 and CD69 expression, T cell proliferation and the release of IFNγ and TNFα. The inhibition on T cell activation by OECs was cell-contact dependent, TGFβ independent and largely irreversible. Overall, this behavior of OECs is likely key to avoid immune system over-reaction against resident bacteria.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-06-28
2019
2019-06-28
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/116159
url https://hdl.handle.net/20.500.14352/116159
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers
publisher.none.fl_str_mv Frontiers
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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