Detection of specific IgA antibodies against a novel deamidated 8-Mer gliadin peptide in blood plasma samples from celiac patients

Producción Científica

Detalhes bibliográficos
Autores: Vallejo Diez, Sara, Bernardo Ordiz, David, Moreno, María de Lourdes, Muñoz Suano, Alba, Fernández Salazar, Luis Ignacio, Calvo Romero, María Del Carmen, Sousa, Carolina, Garrote Adrados, José Antonio, Cebolla, Ángel, Arranz Sanz, Eduardo
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:España
Recursos:Universidad de Valladolid
Repositorio:UVaDOC. Repositorio Documental de la Universidad de Valladolid
OAI Identifier:oai:uvadoc.uva.es:10324/41885
Acesso em linha:https://doi.org/10.1371/journal.pone.0080982
http://uvadoc.uva.es/handle/10324/41885
Access Level:acceso abierto
Palavra-chave:Anticuerpos
Enfermedad celíaca
3205 Medicina Interna
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spelling Detection of specific IgA antibodies against a novel deamidated 8-Mer gliadin peptide in blood plasma samples from celiac patientsVallejo Diez, SaraBernardo Ordiz, DavidMoreno, María de LourdesMuñoz Suano, AlbaFernández Salazar, Luis IgnacioCalvo Romero, María Del CarmenSousa, CarolinaGarrote Adrados, José AntonioCebolla, ÁngelArranz Sanz, EduardoAnticuerposEnfermedad celíaca3205 Medicina InternaProducción CientíficaWe studied whether celiac disease (CD) patients produce antibodies against a novel gliadin peptide specifically generated in the duodenum of CD patients by a previously described pattern of CD-specific duodenal proteases. Fingerprinting and ion-trap mass spectrometry of CD-specific duodenal gliadin-degrading protease pattern revealed a new 8-mer gliadin-derived peptide. An ELISA against synthetic deamidated 8-mer peptides (DGP 8-mer) was used to study the presence of IgA anti-DGP 8-mer antibodies in plasma samples from 81 children (31 active CD patients (aCD), 17 CD patients on a gluten-free diet (GFD), 10 healthy controls (C) and 23 patients with other gastrointestinal pathology (GP)) and 101 adults (16 aCD, 12 GFD, 27 C and 46 GP-patients). Deamidation of the 8-mer peptide significantly increased the reactivity of the IgA antibodies from CD patients against the peptide. Significant IgA anti-DGP 8-mer antibodies levels were detected in 93.5% of aCD-, 11.8% of GFD- and 4.3% of GP-patients in children. In adults, antibodies were detected in 81.3% of aCD-patients and 8.3% of GFD-patients while were absent in 100% of C- and GP-patients. Duodenal CD-specific gliadin degrading proteases release an 8-mer gliadin peptide that once deamidated is an antigen for specific IgA antibodies in CD patients which may provide a new accurate diagnostic tool in CD.Public Library of Science2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://doi.org/10.1371/journal.pone.0080982http://uvadoc.uva.es/handle/10324/41885reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolidinstname:Universidad de ValladolidIngléshttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0080982info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/oai:uvadoc.uva.es:10324/418852026-06-13T12:44:47Z
dc.title.none.fl_str_mv Detection of specific IgA antibodies against a novel deamidated 8-Mer gliadin peptide in blood plasma samples from celiac patients
title Detection of specific IgA antibodies against a novel deamidated 8-Mer gliadin peptide in blood plasma samples from celiac patients
spellingShingle Detection of specific IgA antibodies against a novel deamidated 8-Mer gliadin peptide in blood plasma samples from celiac patients
Vallejo Diez, Sara
Anticuerpos
Enfermedad celíaca
3205 Medicina Interna
title_short Detection of specific IgA antibodies against a novel deamidated 8-Mer gliadin peptide in blood plasma samples from celiac patients
title_full Detection of specific IgA antibodies against a novel deamidated 8-Mer gliadin peptide in blood plasma samples from celiac patients
title_fullStr Detection of specific IgA antibodies against a novel deamidated 8-Mer gliadin peptide in blood plasma samples from celiac patients
title_full_unstemmed Detection of specific IgA antibodies against a novel deamidated 8-Mer gliadin peptide in blood plasma samples from celiac patients
title_sort Detection of specific IgA antibodies against a novel deamidated 8-Mer gliadin peptide in blood plasma samples from celiac patients
dc.creator.none.fl_str_mv Vallejo Diez, Sara
Bernardo Ordiz, David
Moreno, María de Lourdes
Muñoz Suano, Alba
Fernández Salazar, Luis Ignacio
Calvo Romero, María Del Carmen
Sousa, Carolina
Garrote Adrados, José Antonio
Cebolla, Ángel
Arranz Sanz, Eduardo
author Vallejo Diez, Sara
author_facet Vallejo Diez, Sara
Bernardo Ordiz, David
Moreno, María de Lourdes
Muñoz Suano, Alba
Fernández Salazar, Luis Ignacio
Calvo Romero, María Del Carmen
Sousa, Carolina
Garrote Adrados, José Antonio
Cebolla, Ángel
Arranz Sanz, Eduardo
author_role author
author2 Bernardo Ordiz, David
Moreno, María de Lourdes
Muñoz Suano, Alba
Fernández Salazar, Luis Ignacio
Calvo Romero, María Del Carmen
Sousa, Carolina
Garrote Adrados, José Antonio
Cebolla, Ángel
Arranz Sanz, Eduardo
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Anticuerpos
Enfermedad celíaca
3205 Medicina Interna
topic Anticuerpos
Enfermedad celíaca
3205 Medicina Interna
description Producción Científica
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1371/journal.pone.0080982
http://uvadoc.uva.es/handle/10324/41885
url https://doi.org/10.1371/journal.pone.0080982
http://uvadoc.uva.es/handle/10324/41885
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0080982
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolid
instname:Universidad de Valladolid
instname_str Universidad de Valladolid
reponame_str UVaDOC. Repositorio Documental de la Universidad de Valladolid
collection UVaDOC. Repositorio Documental de la Universidad de Valladolid
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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