Putrescine production via the agmatine deiminase pathway increases the growth of Lactococcus lactis and causes the alkalinization of the culture medium

© 2014, Springer-Verlag Berlin Heidelberg. Lactococcus lactis is the most important starter culture organism used in the dairy industry. Although L. lactis species have been awarded Qualified Presumption of Safety status by the European Food Safety Authority, and Generally Regarded as Safe status by...

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Autores: Río Lagar, Beatriz del, Linares, Daniel M., Ladero Losada, Víctor Manuel, Redruello, Begoña, Fernández García, María, Martín, M. Cruz, Álvarez González, Miguel Ángel
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2015
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:dnet:digitalcsic_::57ad3e959d853af8e26bdb8cb1eeee51
Acceso en línea:http://hdl.handle.net/10261/124805
Access Level:acceso abierto
Palabra clave:Biogenic amines
Putrescine
Agmatine deiminase
AGDI cluster
Bacterial growth
Lactococcus lactis
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spelling Putrescine production via the agmatine deiminase pathway increases the growth of Lactococcus lactis and causes the alkalinization of the culture mediumRío Lagar, Beatriz delLinares, Daniel M.Ladero Losada, Víctor ManuelRedruello, BegoñaFernández García, MaríaMartín, M. CruzÁlvarez González, Miguel ÁngelBiogenic aminesPutrescineAgmatine deiminaseAGDI clusterBacterial growthLactococcus lactis© 2014, Springer-Verlag Berlin Heidelberg. Lactococcus lactis is the most important starter culture organism used in the dairy industry. Although L. lactis species have been awarded Qualified Presumption of Safety status by the European Food Safety Authority, and Generally Regarded as Safe status by the US Food and Drug Administration, some strains can produce the biogenic amine putrescine. One such strain is L. lactis subsp. cremoris CECT 8666 (formerly L. lactis subsp. cremoris GE2-14), which was isolated from Genestoso cheese. This strain catabolizes agmatine to putrescine via the agmatine deiminase (AGDI) pathway, which involves the production of ATP and two ammonium ions. The present work shows that the availability of agmatine and its metabolization to putrescine allows for greater bacterial growth (in a biphasic pattern) and causes the alkalinization of the culture medium in a dose-dependent manner. The construction of a mutant lacking the AGDI cluster (L. lactis CECT 8666 Δagdi) confirmed the latter’s direct role in putrescine production, growth, and medium alkalinization. Alkalinization did not affect the putrescine production pattern and was not essential for increased bacterial growth.This work was performed with the financial support of the Spanish Ministry of Economy and Competitiveness (AGL2013-45431-R) and the CSIC (201270E144). B. del Rio and D. Linares were beneficiaries of a JAE DOC contract (CSIC).Peer ReviewedSpringer NatureEuropean CommissionMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2015201520152015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/124805reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2013-45431-Rhttp://dx.doi.org/10.1007/s00253-014-6130-8Síinfo:eu-repo/semantics/openAccessoai:dnet:digitalcsic_::57ad3e959d853af8e26bdb8cb1eeee512026-05-22T06:33:51Z
dc.title.none.fl_str_mv Putrescine production via the agmatine deiminase pathway increases the growth of Lactococcus lactis and causes the alkalinization of the culture medium
title Putrescine production via the agmatine deiminase pathway increases the growth of Lactococcus lactis and causes the alkalinization of the culture medium
spellingShingle Putrescine production via the agmatine deiminase pathway increases the growth of Lactococcus lactis and causes the alkalinization of the culture medium
Río Lagar, Beatriz del
Biogenic amines
Putrescine
Agmatine deiminase
AGDI cluster
Bacterial growth
Lactococcus lactis
title_short Putrescine production via the agmatine deiminase pathway increases the growth of Lactococcus lactis and causes the alkalinization of the culture medium
title_full Putrescine production via the agmatine deiminase pathway increases the growth of Lactococcus lactis and causes the alkalinization of the culture medium
title_fullStr Putrescine production via the agmatine deiminase pathway increases the growth of Lactococcus lactis and causes the alkalinization of the culture medium
title_full_unstemmed Putrescine production via the agmatine deiminase pathway increases the growth of Lactococcus lactis and causes the alkalinization of the culture medium
title_sort Putrescine production via the agmatine deiminase pathway increases the growth of Lactococcus lactis and causes the alkalinization of the culture medium
dc.creator.none.fl_str_mv Río Lagar, Beatriz del
Linares, Daniel M.
Ladero Losada, Víctor Manuel
Redruello, Begoña
Fernández García, María
Martín, M. Cruz
Álvarez González, Miguel Ángel
author Río Lagar, Beatriz del
author_facet Río Lagar, Beatriz del
Linares, Daniel M.
Ladero Losada, Víctor Manuel
Redruello, Begoña
Fernández García, María
Martín, M. Cruz
Álvarez González, Miguel Ángel
author_role author
author2 Linares, Daniel M.
Ladero Losada, Víctor Manuel
Redruello, Begoña
Fernández García, María
Martín, M. Cruz
Álvarez González, Miguel Ángel
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Biogenic amines
Putrescine
Agmatine deiminase
AGDI cluster
Bacterial growth
Lactococcus lactis
topic Biogenic amines
Putrescine
Agmatine deiminase
AGDI cluster
Bacterial growth
Lactococcus lactis
description © 2014, Springer-Verlag Berlin Heidelberg. Lactococcus lactis is the most important starter culture organism used in the dairy industry. Although L. lactis species have been awarded Qualified Presumption of Safety status by the European Food Safety Authority, and Generally Regarded as Safe status by the US Food and Drug Administration, some strains can produce the biogenic amine putrescine. One such strain is L. lactis subsp. cremoris CECT 8666 (formerly L. lactis subsp. cremoris GE2-14), which was isolated from Genestoso cheese. This strain catabolizes agmatine to putrescine via the agmatine deiminase (AGDI) pathway, which involves the production of ATP and two ammonium ions. The present work shows that the availability of agmatine and its metabolization to putrescine allows for greater bacterial growth (in a biphasic pattern) and causes the alkalinization of the culture medium in a dose-dependent manner. The construction of a mutant lacking the AGDI cluster (L. lactis CECT 8666 Δagdi) confirmed the latter’s direct role in putrescine production, growth, and medium alkalinization. Alkalinization did not affect the putrescine production pattern and was not essential for increased bacterial growth.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/124805
url http://hdl.handle.net/10261/124805
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2013-45431-R
http://dx.doi.org/10.1007/s00253-014-6130-8
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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