Recovery of lactose and proteins from cheese whey with poly(ethylene)glycol/sulfate aqueous two-phase systems

Cheese whey is an environmental problem as an effluent, but also a source of valuable raw materials, namely proteins and lactose. In this work, Aqueous Two-Phase Systems (ATPS) were studied for the recovery of lactose, BSA, β-lactoglobulin and α-lactalbumin, key components of cheese whey. ATPS forme...

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Detalles Bibliográficos
Autores: González Amado, Marlen, Tavares, Ana Paula Mora, Freire, Mara G., Soto Campos, Ana María, Rodríguez Figueiras, Óscar
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad de Santiago de Compostela (USC)
Repositorio:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
Idioma:inglés
OAI Identifier:oai:minerva.usc.gal:10347/44223
Acceso en línea:https://hdl.handle.net/10347/44223
Access Level:acceso abierto
Palabra clave:Aqueous two-phase systems
Cheese whey
Bovine serum albumin
β-lactoglobulin
α-lactalbumin
Separation
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spelling Recovery of lactose and proteins from cheese whey with poly(ethylene)glycol/sulfate aqueous two-phase systemsGonzález Amado, MarlenTavares, Ana Paula MoraFreire, Mara G.Soto Campos, Ana MaríaRodríguez Figueiras, ÓscarAqueous two-phase systemsCheese wheyBovine serum albuminβ-lactoglobulinα-lactalbuminSeparationCheese whey is an environmental problem as an effluent, but also a source of valuable raw materials, namely proteins and lactose. In this work, Aqueous Two-Phase Systems (ATPS) were studied for the recovery of lactose, BSA, β-lactoglobulin and α-lactalbumin, key components of cheese whey. ATPS formed by PEG (molecular weights: 200–8000 g.mol−1) with sodium or ammonium sulfate were investigated. Partitioning of the selected solutes was experimentally addressed in different ATPS and pH values. Partition behavior showed that ATPS formed by PEG1500/ammonium sulfate is able to separate lactose from proteins, while PEG300/sodium sulfate ATPS may be used for protein fractionation. These separation strategies were then tested with simulated and real cheese whey. Under optimized conditions, PEG 1500/ammonium sulfate ATPS allows efficient recovery of > 95% proteins (precipitate) and 80% of lactose (bottom phase), as confirmed for both simulated and real cheese whey. The results found indicate that the proposed polymer/salt ATPS can be used to design scalable and cost-effective separation strategies to apply in cheese whey and other related wastes.ElsevierUniversidade de Santiago de Compostela. Departamento de Enxeñaría QuímicaUniversidade de Santiago de Compostela. Centro Interdisciplinar de Investigación en Tecnoloxías Ambientais (CRETUS)20202020-09-0120202020-09-01journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10347/44223reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostelainstname:Universidad de Santiago de Compostela (USC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:minerva.usc.gal:10347/442232026-06-15T12:47:27Z
dc.title.none.fl_str_mv Recovery of lactose and proteins from cheese whey with poly(ethylene)glycol/sulfate aqueous two-phase systems
title Recovery of lactose and proteins from cheese whey with poly(ethylene)glycol/sulfate aqueous two-phase systems
spellingShingle Recovery of lactose and proteins from cheese whey with poly(ethylene)glycol/sulfate aqueous two-phase systems
González Amado, Marlen
Aqueous two-phase systems
Cheese whey
Bovine serum albumin
β-lactoglobulin
α-lactalbumin
Separation
title_short Recovery of lactose and proteins from cheese whey with poly(ethylene)glycol/sulfate aqueous two-phase systems
title_full Recovery of lactose and proteins from cheese whey with poly(ethylene)glycol/sulfate aqueous two-phase systems
title_fullStr Recovery of lactose and proteins from cheese whey with poly(ethylene)glycol/sulfate aqueous two-phase systems
title_full_unstemmed Recovery of lactose and proteins from cheese whey with poly(ethylene)glycol/sulfate aqueous two-phase systems
title_sort Recovery of lactose and proteins from cheese whey with poly(ethylene)glycol/sulfate aqueous two-phase systems
dc.creator.none.fl_str_mv González Amado, Marlen
Tavares, Ana Paula Mora
Freire, Mara G.
Soto Campos, Ana María
Rodríguez Figueiras, Óscar
author González Amado, Marlen
author_facet González Amado, Marlen
Tavares, Ana Paula Mora
Freire, Mara G.
Soto Campos, Ana María
Rodríguez Figueiras, Óscar
author_role author
author2 Tavares, Ana Paula Mora
Freire, Mara G.
Soto Campos, Ana María
Rodríguez Figueiras, Óscar
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade de Santiago de Compostela. Departamento de Enxeñaría Química
Universidade de Santiago de Compostela. Centro Interdisciplinar de Investigación en Tecnoloxías Ambientais (CRETUS)
dc.subject.none.fl_str_mv Aqueous two-phase systems
Cheese whey
Bovine serum albumin
β-lactoglobulin
α-lactalbumin
Separation
topic Aqueous two-phase systems
Cheese whey
Bovine serum albumin
β-lactoglobulin
α-lactalbumin
Separation
description Cheese whey is an environmental problem as an effluent, but also a source of valuable raw materials, namely proteins and lactose. In this work, Aqueous Two-Phase Systems (ATPS) were studied for the recovery of lactose, BSA, β-lactoglobulin and α-lactalbumin, key components of cheese whey. ATPS formed by PEG (molecular weights: 200–8000 g.mol−1) with sodium or ammonium sulfate were investigated. Partitioning of the selected solutes was experimentally addressed in different ATPS and pH values. Partition behavior showed that ATPS formed by PEG1500/ammonium sulfate is able to separate lactose from proteins, while PEG300/sodium sulfate ATPS may be used for protein fractionation. These separation strategies were then tested with simulated and real cheese whey. Under optimized conditions, PEG 1500/ammonium sulfate ATPS allows efficient recovery of > 95% proteins (precipitate) and 80% of lactose (bottom phase), as confirmed for both simulated and real cheese whey. The results found indicate that the proposed polymer/salt ATPS can be used to design scalable and cost-effective separation strategies to apply in cheese whey and other related wastes.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-09-01
2020
2020-09-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10347/44223
url https://hdl.handle.net/10347/44223
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
instname:Universidad de Santiago de Compostela (USC)
instname_str Universidad de Santiago de Compostela (USC)
reponame_str Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
collection Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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