A Novel Column Format for on-Chip Liquid Chromatography Fabricated in Cyclo Olefin Polymer

This thesis describes a novel column format to be used in pressure-driven reversed-phase liquid chromatography. The column contains an ordered pillar array where the stationary phase is supported, and it is fabricated in cyclo olefin polymer (COP) by means of standard replication techniques. The pil...

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Autor: Illa Vila, Xavier
Tipo de recurso: tesis doctoral
Estado:Versión publicada
Fecha de publicación:2010
País:España
Institución:CBUC, CESCA
Repositorio:TDR. Tesis Doctorales en Red
OAI Identifier:oai:www.tdx.cat:10803/1518
Acceso en línea:http://www.tdx.cat/TDX-1104110-104016
http://hdl.handle.net/10803/1518
Access Level:acceso abierto
Palabra clave:Cromatografia de líquids
Electrònica
Ciències Experimentals i Matemàtiques
53
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oai_identifier_str oai:www.tdx.cat:10803/1518
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network_name_str España
repository_id_str
dc.title.none.fl_str_mv A Novel Column Format for on-Chip Liquid Chromatography Fabricated in Cyclo Olefin Polymer
title A Novel Column Format for on-Chip Liquid Chromatography Fabricated in Cyclo Olefin Polymer
spellingShingle A Novel Column Format for on-Chip Liquid Chromatography Fabricated in Cyclo Olefin Polymer
Illa Vila, Xavier
Cromatografia de líquids
Electrònica
Ciències Experimentals i Matemàtiques
53
title_short A Novel Column Format for on-Chip Liquid Chromatography Fabricated in Cyclo Olefin Polymer
title_full A Novel Column Format for on-Chip Liquid Chromatography Fabricated in Cyclo Olefin Polymer
title_fullStr A Novel Column Format for on-Chip Liquid Chromatography Fabricated in Cyclo Olefin Polymer
title_full_unstemmed A Novel Column Format for on-Chip Liquid Chromatography Fabricated in Cyclo Olefin Polymer
title_sort A Novel Column Format for on-Chip Liquid Chromatography Fabricated in Cyclo Olefin Polymer
dc.creator.none.fl_str_mv Illa Vila, Xavier
author Illa Vila, Xavier
author_facet Illa Vila, Xavier
author_role author
dc.contributor.none.fl_str_mv Romano Rodríguez, Albert
De Malsche, Wim
Universitat de Barcelona. Departament d'Electrònica
dc.subject.none.fl_str_mv Cromatografia de líquids
Electrònica
Ciències Experimentals i Matemàtiques
53
topic Cromatografia de líquids
Electrònica
Ciències Experimentals i Matemàtiques
53
description This thesis describes a novel column format to be used in pressure-driven reversed-phase liquid chromatography. The column contains an ordered pillar array where the stationary phase is supported, and it is fabricated in cyclo olefin polymer (COP) by means of standard replication techniques. The pillar array column format has shown much better efficiencies than the other traditional liquid chromatography column formats due to the order achieved with the controlled pillar packing. A particularity of this work is that the unmodified surface of the pillars acts directly as the stationary phase due to the high hydrophobicity of the COP.<br/><br/>To evaluate the performance of the fabricated column, two different experimental studies concerning the efficiency and the retention properties have been carried out using columns with the same pillar structure but with three different depths. In the first one, the influence of the depth on the band broadening effect along with the extra contribution arising from the top and bottom cover plates has been studied by injection and fluorescence detection of coumarins. In the second study, separations of different coumarin mixtures using varying mobile phase compositions have been monitored to study i) the relation between the retention factor and the ratio of organic solvent in the aqueous mobile phase and, ii) the linear relation between the retention and the surface/volume ratio, which are both predicted in the literature. The results of both studies have been compared to the case of the pillar array column fabricated in non-porous silicon showing even better results for both cases which indicates the potential this polymer column as an alternative to the pillar array silicon columns. <br/><br/>This thesis also describes the fabrication process of gold microelectrodes on the surface of the COP. In addition, the microelectrodes have been integrated into a microchannel fabricated also in COP in order to validate its response. In particular, voltammetric measurements have been performed in aqueous and organic (for the first time) media. The obtained results have been compared with the theory showing an almost perfect agreement, which open the door for the possibility to use a microfluidic system entirely made from COP with integrated microband electrodes in organic electroanalysis and in electrosynthesis.<br/><br/>Finally, a first step in order to replace the complex optical detection system used in the pillar array column characterization by a more simple electrochemical detection system is described. For that, first results on the integration of the fabricated gold microelectrodes at the end of the COP pillar array column are given at the end of this thesis.<br/><br/>KEY WORDS: On-Chip Liquid Chromatography, Cyclo Olefin Polymer, Pillar array column, Gold microelectrodes
publishDate 2010
dc.date.none.fl_str_mv 2010
2010
2010
2011
dc.type.none.fl_str_mv info:eu-repo/semantics/doctoralThesis
info:eu-repo/semantics/publishedVersion
format doctoralThesis
status_str publishedVersion
dc.identifier.none.fl_str_mv http://www.tdx.cat/TDX-1104110-104016
http://hdl.handle.net/10803/1518
url http://www.tdx.cat/TDX-1104110-104016
http://hdl.handle.net/10803/1518
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universitat de Barcelona
publisher.none.fl_str_mv Universitat de Barcelona
dc.source.none.fl_str_mv TDX (Tesis Doctorals en Xarxa)
reponame:TDR. Tesis Doctorales en Red
instname:CBUC, CESCA
instname_str CBUC, CESCA
reponame_str TDR. Tesis Doctorales en Red
collection TDR. Tesis Doctorales en Red
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling A Novel Column Format for on-Chip Liquid Chromatography Fabricated in Cyclo Olefin PolymerIlla Vila, XavierCromatografia de líquidsElectrònicaCiències Experimentals i Matemàtiques53This thesis describes a novel column format to be used in pressure-driven reversed-phase liquid chromatography. The column contains an ordered pillar array where the stationary phase is supported, and it is fabricated in cyclo olefin polymer (COP) by means of standard replication techniques. The pillar array column format has shown much better efficiencies than the other traditional liquid chromatography column formats due to the order achieved with the controlled pillar packing. A particularity of this work is that the unmodified surface of the pillars acts directly as the stationary phase due to the high hydrophobicity of the COP.<br/><br/>To evaluate the performance of the fabricated column, two different experimental studies concerning the efficiency and the retention properties have been carried out using columns with the same pillar structure but with three different depths. In the first one, the influence of the depth on the band broadening effect along with the extra contribution arising from the top and bottom cover plates has been studied by injection and fluorescence detection of coumarins. In the second study, separations of different coumarin mixtures using varying mobile phase compositions have been monitored to study i) the relation between the retention factor and the ratio of organic solvent in the aqueous mobile phase and, ii) the linear relation between the retention and the surface/volume ratio, which are both predicted in the literature. The results of both studies have been compared to the case of the pillar array column fabricated in non-porous silicon showing even better results for both cases which indicates the potential this polymer column as an alternative to the pillar array silicon columns. <br/><br/>This thesis also describes the fabrication process of gold microelectrodes on the surface of the COP. In addition, the microelectrodes have been integrated into a microchannel fabricated also in COP in order to validate its response. In particular, voltammetric measurements have been performed in aqueous and organic (for the first time) media. The obtained results have been compared with the theory showing an almost perfect agreement, which open the door for the possibility to use a microfluidic system entirely made from COP with integrated microband electrodes in organic electroanalysis and in electrosynthesis.<br/><br/>Finally, a first step in order to replace the complex optical detection system used in the pillar array column characterization by a more simple electrochemical detection system is described. For that, first results on the integration of the fabricated gold microelectrodes at the end of the COP pillar array column are given at the end of this thesis.<br/><br/>KEY WORDS: On-Chip Liquid Chromatography, Cyclo Olefin Polymer, Pillar array column, Gold microelectrodes<i>En aquesta tesi es descriu un nou format de columna per a ser utilitzat en cromatografia de líquids i feta en polímer de cicloolefina (de l'anglès, COP). La columna està fabricada per mètodes litogràfics de replicació en calent i conté una matriu de pilars ordenats per subjectar la fase estacionària, que simplement consisteix en la mateixa superfície no modificada dels pilars. Això és possible degut a l'alta hidrofobicitat del polímer emprat per a la fabricació del dispositiu.<br/><br/>La injecció i detecció per fluorescència de cumarines s'ha fet servir, en una primera instància, per analitzar l'eficiència de la columna. En segon lloc, l'anàlisi de les propietats retentives de la columna s'ha realitzat mitjançant l'estudi de les separacions de barreges de diferents tipus de cumarines.<br/><br/>En aquest treball també es descriu el desenvolupament i optimització del procés per a la fabricació de microelèctrodes d'or sobre la superfície del COP. Així mateix, els microelèctrodes s'han integrat dins d'un microcanal fabricat també en COP per a la validació de la seva resposta elèctrica en medi aquós i, per primera vegada, en medi orgànic. Per últim, s'ha estudiat la possibilitat d'integrar els microelèctrodes a la sortida de la columna cromatogràfica fabricada en COP per tal de substituir el complex sistema de detecció òptica per un de més simple basat en la detecció elèctrica dels analits.</I>Universitat de BarcelonaRomano Rodríguez, AlbertDe Malsche, WimUniversitat de Barcelona. Departament d'Electrònica2011201020102010info:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://www.tdx.cat/TDX-1104110-104016http://hdl.handle.net/10803/1518TDX (Tesis Doctorals en Xarxa)reponame:TDR. Tesis Doctorales en Redinstname:CBUC, CESCAInglésADVERTIMENT. L'accés als continguts d'aquesta tesi doctoral i la seva utilització ha de respectar els drets de la persona autora. Pot ser utilitzada per a consulta o estudi personal, així com en activitats o materials d'investigació i docència en els termes establerts a l'art. 32 del Text Refós de la Llei de Propietat Intel·lectual (RDL 1/1996). Per altres utilitzacions es requereix l'autorització prèvia i expressa de la persona autora. En qualsevol cas, en la utilització dels seus continguts caldrà indicar de forma clara el nom i cognoms de la persona autora i el títol de la tesi doctoral. No s'autoritza la seva reproducció o altres formes d'explotació efectuades amb finalitats de lucre ni la seva comunicació pública des d'un lloc aliè al servei TDX. Tampoc s'autoritza la presentació del seu contingut en una finestra o marc aliè a TDX (framing). Aquesta reserva de drets afecta tant als continguts de la tesi com als seus resums i índexs.info:eu-repo/semantics/openAccessoai:www.tdx.cat:10803/15182026-06-14T12:46:07Z
score 15.300719

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