Genome-wide characterization of a viral cytotoxic T lymphocyte epitope repertoire

A genome-wide search using major histocompatibility complex (MHC) class I binding and proteosome cleavage site algorithms identified 101 influenza A PR8 virus-derived peptides as potential epitopes for CD8+ T cell recognition in the H-2b mouse. Cytokine-based flow cytometry, ELISPOT, and cytotoxic T...

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Detalles Bibliográficos
Autores: Zhong, Weimin, Reche Gallardo, Pedro Antonio, Lai, Char-Chang, Reinhold, Bruce, Reinherz, Ellis L
Tipo de recurso: artículo
Fecha de publicación:2003
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/58244
Acceso en línea:https://hdl.handle.net/20.500.14352/58244
Access Level:acceso abierto
Palabra clave:Inmunología
Microbiología (Biología)
Bioinformática
Biología molecular (Biología)
2412 Inmunología
2414 Microbiología
2415 Biología Molecular
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spelling Genome-wide characterization of a viral cytotoxic T lymphocyte epitope repertoireZhong, WeiminReche Gallardo, Pedro AntonioLai, Char-ChangReinhold, BruceReinherz, Ellis LInmunologíaMicrobiología (Biología)BioinformáticaBiología molecular (Biología)2412 Inmunología2414 Microbiología2415 Biología MolecularA genome-wide search using major histocompatibility complex (MHC) class I binding and proteosome cleavage site algorithms identified 101 influenza A PR8 virus-derived peptides as potential epitopes for CD8+ T cell recognition in the H-2b mouse. Cytokine-based flow cytometry, ELISPOT, and cytotoxic T lymphocyte assays reveal that 16 are recognized by CD8+ T cells recovered directly ex vivo from infected animals, accounting for greater than 70% of CD8+ T cells recruited to lung after primary infection. Only six of the 22 highest affinity MHC class I binding peptides comprise cytotoxic T lymphocyte epitopes. The remaining non-immunogenic peptides have equivalent MHC affinity and MHC-peptide complex half-lives, eliciting T cell responses when given in adjuvant and with T cell receptor-ligand avidity comparable with their immunogenic counterparts. As revealed by a novel high sensitivity nanospray tandem mass spectrometry methodology, failure to process those predicted epitopes may contribute significantly to the absent response. These results have important implications for rationale design of CD8+ T cell vaccines.American Society for Biochemistry and Molecular BiologyUniversidad Complutense de Madrid20032003-01-0120032003-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/58244reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/582442026-06-02T12:44:21Z
dc.title.none.fl_str_mv Genome-wide characterization of a viral cytotoxic T lymphocyte epitope repertoire
title Genome-wide characterization of a viral cytotoxic T lymphocyte epitope repertoire
spellingShingle Genome-wide characterization of a viral cytotoxic T lymphocyte epitope repertoire
Zhong, Weimin
Inmunología
Microbiología (Biología)
Bioinformática
Biología molecular (Biología)
2412 Inmunología
2414 Microbiología
2415 Biología Molecular
title_short Genome-wide characterization of a viral cytotoxic T lymphocyte epitope repertoire
title_full Genome-wide characterization of a viral cytotoxic T lymphocyte epitope repertoire
title_fullStr Genome-wide characterization of a viral cytotoxic T lymphocyte epitope repertoire
title_full_unstemmed Genome-wide characterization of a viral cytotoxic T lymphocyte epitope repertoire
title_sort Genome-wide characterization of a viral cytotoxic T lymphocyte epitope repertoire
dc.creator.none.fl_str_mv Zhong, Weimin
Reche Gallardo, Pedro Antonio
Lai, Char-Chang
Reinhold, Bruce
Reinherz, Ellis L
author Zhong, Weimin
author_facet Zhong, Weimin
Reche Gallardo, Pedro Antonio
Lai, Char-Chang
Reinhold, Bruce
Reinherz, Ellis L
author_role author
author2 Reche Gallardo, Pedro Antonio
Lai, Char-Chang
Reinhold, Bruce
Reinherz, Ellis L
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv Inmunología
Microbiología (Biología)
Bioinformática
Biología molecular (Biología)
2412 Inmunología
2414 Microbiología
2415 Biología Molecular
topic Inmunología
Microbiología (Biología)
Bioinformática
Biología molecular (Biología)
2412 Inmunología
2414 Microbiología
2415 Biología Molecular
description A genome-wide search using major histocompatibility complex (MHC) class I binding and proteosome cleavage site algorithms identified 101 influenza A PR8 virus-derived peptides as potential epitopes for CD8+ T cell recognition in the H-2b mouse. Cytokine-based flow cytometry, ELISPOT, and cytotoxic T lymphocyte assays reveal that 16 are recognized by CD8+ T cells recovered directly ex vivo from infected animals, accounting for greater than 70% of CD8+ T cells recruited to lung after primary infection. Only six of the 22 highest affinity MHC class I binding peptides comprise cytotoxic T lymphocyte epitopes. The remaining non-immunogenic peptides have equivalent MHC affinity and MHC-peptide complex half-lives, eliciting T cell responses when given in adjuvant and with T cell receptor-ligand avidity comparable with their immunogenic counterparts. As revealed by a novel high sensitivity nanospray tandem mass spectrometry methodology, failure to process those predicted epitopes may contribute significantly to the absent response. These results have important implications for rationale design of CD8+ T cell vaccines.
publishDate 2003
dc.date.none.fl_str_mv 2003
2003-01-01
2003
2003-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/58244
url https://hdl.handle.net/20.500.14352/58244
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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