Spindle configuration of in vitro matured bovine oocytes vitrified and warmed in media supplemented with a biopolymer produced by an Antarctic bacterium

Biological molecules isolated from organisms that live under subfreezing conditions could be used to protect oocytes from cryoinjuries suffered during cryopreservation. Bacterial exopolysaccharides (EPS) constitute a common class of molecules that interact with ice in nature either by triggering ice...

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Autores: Arcarons, N., Vendrell, M., Yeste Velasco, Marc, Mercadé Gil, M. Elena, Mogas, Teresa
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/168617
Acceso en línea:https://hdl.handle.net/2445/168617
Access Level:acceso abierto
Palabra clave:Vitrificació
Cromosomes
Vitrification
Chromosomes
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spelling Spindle configuration of in vitro matured bovine oocytes vitrified and warmed in media supplemented with a biopolymer produced by an Antarctic bacteriumArcarons, N.Vendrell, M.Yeste Velasco, MarcMercadé Gil, M. ElenaMogas, TeresaVitrificacióCromosomesVitrificationChromosomesBiological molecules isolated from organisms that live under subfreezing conditions could be used to protect oocytes from cryoinjuries suffered during cryopreservation. Bacterial exopolysaccharides (EPS) constitute a common class of molecules that interact with ice in nature either by triggering ice nucleation or by inhibition of ice nucleation and growth. The aim of this work was to evaluate the spindle configuration of in vitro matured bovine oocytes vitrified/warmed in media supplemented with exopolysaccharide (M1) produced by Pseudomonas sp ID1 (Carrión et al., Carbohydr Polym 117:1028. 2015). After 22 h of in vitro maturation, a total of 546 oocytes form prepubertal (3 replicates) and 405 oocytes from adult cows (4 replicates) were vitrified/warmed in media supplemented with various concentrations of EPS M1 (0, 0.001, 0.01, 0.1 and 1 mg/ml). After warming, oocytes were allowed to recover for 2 additional hours in IVM medium. Fresh, non-vitrified oocytes were used as a control. At 24 h of IVM, oocytes from all treatments were fixed and immunostained with the Alexa-fluor 488 antibody and DAPI. Microtubule and chromosome distribution was analyzed by immunocytochemistry under a fluorescent microscope. ANOVA was performed to analyze differences in meiotic spindle configuration (P < 0.05). When cow oocytes were vitrified, similar percentages of normal spindle configuration were observed when compared to fresh control oocytes, except for the 0.1 mg/ml EPS M1 group that showed significantly lower rates compared to the fresh control group. Significantly higher rates of prepubertal oocytes exhibiting a normal spindle configuration were recorded in the non-vitrified group compared to all vitrified/warmed groups, regardless of the EPS M1 supplementation. However, the addition of EPS M1 to the vitrification/warming media decreased the ratio of decondensation or absence of chromosomes and microtubules in prepubertal oocytes. Although percentages of normal spindle configuration after vitrification were lower for prepubertal than for cow oocytes, no significant differences were observed when oocytes were vitrified with 0.001, 0.1 and 1 mg/ml EPS M1. In conclusion, supplementation with EPS M1 concentrations during vitrification and warming did not induce adverse changes in the spindle of bovine oocytes, regardless of the concentration used. Although a more severe damage on spindle configuration could be observed after vitrification of prepubertal oocytes, EPS supplementation during vitrification and warming seems to have a greater benefit during vitrification of prepubertal than adult bovine oocytes. Further experiments are required to investigate if in vitro-matured oocytes vitrified/warmed in presence EPS M1 can improve their development competence after being vitrified/warmed. This study was supported by the Spanish Ministry of Science and Innovation (Project AGL2016-79802-P and grant CTQ2014-59632-R).Colégio Brasileiro de Reprodução Animal2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/168617Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: http://www.cbra.org.br/portal/downloads/publicacoes/animalreproduction/issues/download/v14/v14n3/p970-984%20(Support%20biotechnologies..)%20AETE.pdfAnimal Reproduction, 2017, vol. 14, num. 3, p. 970-970cc-by (c) Arcarons, N. et al., 2017http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1686172026-05-27T06:46:51Z
dc.title.none.fl_str_mv Spindle configuration of in vitro matured bovine oocytes vitrified and warmed in media supplemented with a biopolymer produced by an Antarctic bacterium
title Spindle configuration of in vitro matured bovine oocytes vitrified and warmed in media supplemented with a biopolymer produced by an Antarctic bacterium
spellingShingle Spindle configuration of in vitro matured bovine oocytes vitrified and warmed in media supplemented with a biopolymer produced by an Antarctic bacterium
Arcarons, N.
Vitrificació
Cromosomes
Vitrification
Chromosomes
title_short Spindle configuration of in vitro matured bovine oocytes vitrified and warmed in media supplemented with a biopolymer produced by an Antarctic bacterium
title_full Spindle configuration of in vitro matured bovine oocytes vitrified and warmed in media supplemented with a biopolymer produced by an Antarctic bacterium
title_fullStr Spindle configuration of in vitro matured bovine oocytes vitrified and warmed in media supplemented with a biopolymer produced by an Antarctic bacterium
title_full_unstemmed Spindle configuration of in vitro matured bovine oocytes vitrified and warmed in media supplemented with a biopolymer produced by an Antarctic bacterium
title_sort Spindle configuration of in vitro matured bovine oocytes vitrified and warmed in media supplemented with a biopolymer produced by an Antarctic bacterium
dc.creator.none.fl_str_mv Arcarons, N.
Vendrell, M.
Yeste Velasco, Marc
Mercadé Gil, M. Elena
Mogas, Teresa
author Arcarons, N.
author_facet Arcarons, N.
Vendrell, M.
Yeste Velasco, Marc
Mercadé Gil, M. Elena
Mogas, Teresa
author_role author
author2 Vendrell, M.
Yeste Velasco, Marc
Mercadé Gil, M. Elena
Mogas, Teresa
author2_role author
author
author
author
dc.subject.none.fl_str_mv Vitrificació
Cromosomes
Vitrification
Chromosomes
topic Vitrificació
Cromosomes
Vitrification
Chromosomes
description Biological molecules isolated from organisms that live under subfreezing conditions could be used to protect oocytes from cryoinjuries suffered during cryopreservation. Bacterial exopolysaccharides (EPS) constitute a common class of molecules that interact with ice in nature either by triggering ice nucleation or by inhibition of ice nucleation and growth. The aim of this work was to evaluate the spindle configuration of in vitro matured bovine oocytes vitrified/warmed in media supplemented with exopolysaccharide (M1) produced by Pseudomonas sp ID1 (Carrión et al., Carbohydr Polym 117:1028. 2015). After 22 h of in vitro maturation, a total of 546 oocytes form prepubertal (3 replicates) and 405 oocytes from adult cows (4 replicates) were vitrified/warmed in media supplemented with various concentrations of EPS M1 (0, 0.001, 0.01, 0.1 and 1 mg/ml). After warming, oocytes were allowed to recover for 2 additional hours in IVM medium. Fresh, non-vitrified oocytes were used as a control. At 24 h of IVM, oocytes from all treatments were fixed and immunostained with the Alexa-fluor 488 antibody and DAPI. Microtubule and chromosome distribution was analyzed by immunocytochemistry under a fluorescent microscope. ANOVA was performed to analyze differences in meiotic spindle configuration (P < 0.05). When cow oocytes were vitrified, similar percentages of normal spindle configuration were observed when compared to fresh control oocytes, except for the 0.1 mg/ml EPS M1 group that showed significantly lower rates compared to the fresh control group. Significantly higher rates of prepubertal oocytes exhibiting a normal spindle configuration were recorded in the non-vitrified group compared to all vitrified/warmed groups, regardless of the EPS M1 supplementation. However, the addition of EPS M1 to the vitrification/warming media decreased the ratio of decondensation or absence of chromosomes and microtubules in prepubertal oocytes. Although percentages of normal spindle configuration after vitrification were lower for prepubertal than for cow oocytes, no significant differences were observed when oocytes were vitrified with 0.001, 0.1 and 1 mg/ml EPS M1. In conclusion, supplementation with EPS M1 concentrations during vitrification and warming did not induce adverse changes in the spindle of bovine oocytes, regardless of the concentration used. Although a more severe damage on spindle configuration could be observed after vitrification of prepubertal oocytes, EPS supplementation during vitrification and warming seems to have a greater benefit during vitrification of prepubertal than adult bovine oocytes. Further experiments are required to investigate if in vitro-matured oocytes vitrified/warmed in presence EPS M1 can improve their development competence after being vitrified/warmed. This study was supported by the Spanish Ministry of Science and Innovation (Project AGL2016-79802-P and grant CTQ2014-59632-R).
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/168617
url https://hdl.handle.net/2445/168617
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://www.cbra.org.br/portal/downloads/publicacoes/animalreproduction/issues/download/v14/v14n3/p970-984%20(Support%20biotechnologies..)%20AETE.pdf
Animal Reproduction, 2017, vol. 14, num. 3, p. 970-970
dc.rights.none.fl_str_mv cc-by (c) Arcarons, N. et al., 2017
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Arcarons, N. et al., 2017
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Colégio Brasileiro de Reprodução Animal
publisher.none.fl_str_mv Colégio Brasileiro de Reprodução Animal
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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