SLY1 and syntaxin 18 specify a distinct pathway for procollagen VII export from the endoplasmic reticulum

TANGO1 binds and exports Procollagen VII from the endoplasmic reticulum (ER). In this study, we report a connection between the cytoplasmic domain of TANGO1 and SLY1, a protein that is required for membrane fusion. Knockdown of SLY1 by siRNA arrested Procollagen VII in the ER without affecting the r...

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Detalles Bibliográficos
Autores: Nogueira, Cristina, Erlmann, Patrik, Villeneuve, Julien, Santos, António J. M., Martínez-Alonso, Emma, Martínez-Menárguez, José Ángel, Malhotra, Vivek
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Institución:Universitat Pompeu Fabra
Repositorio:Repositorio Digital de la UPF
OAI Identifier:oai:repositori.upf.edu:10230/60190
Acceso en línea:http://hdl.handle.net/10230/60190
http://dx.doi.org/10.7554/eLife.02784
Access Level:acceso abierto
Palabra clave:Reticle endoplasmàtic
Col·lagen
Proteïnes
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spelling SLY1 and syntaxin 18 specify a distinct pathway for procollagen VII export from the endoplasmic reticulumNogueira, CristinaErlmann, PatrikVilleneuve, JulienSantos, António J. M.Martínez-Alonso, EmmaMartínez-Menárguez, José ÁngelMalhotra, VivekReticle endoplasmàticCol·lagenProteïnesTANGO1 binds and exports Procollagen VII from the endoplasmic reticulum (ER). In this study, we report a connection between the cytoplasmic domain of TANGO1 and SLY1, a protein that is required for membrane fusion. Knockdown of SLY1 by siRNA arrested Procollagen VII in the ER without affecting the recruitment of COPII components, general protein secretion, and retrograde transport of the KDEL-containing protein BIP, and ERGIC53. SLY1 is known to interact with the ER-specific SNARE proteins Syntaxin 17 and 18, however only Syntaxin 18 was required for Procollagen VII export. Neither SLY1 nor Syntaxin 18 was required for the export of the equally bulky Procollagen I from the ER. Altogether, these findings reveal the sorting of bulky collagen family members by TANGO1 at the ER and highlight the existence of different export pathways for secretory cargoes one of which is mediated by the specific SNARE complex containing SLY1 and Syntaxin 18.We thank the entire Malhotra Lab for help with figures and corrections. We would like to acknowledge the CRG's mass spectrometry facility for sample analysis and the CRG advanced light microscopy facility for help with conventional and STED fluorescent microscopy. P Erlmann was partially funded by a DFG fellowship (ER 681/1-1). V Malhotra is an Institució Catalana de Recerca i Estudis Avançats (ICREA) professor at the Center for Genomic Regulation and the work in his laboratory is funded by grants from Plan Nacional (BFU2008-00414), Consolider (CSD2009-00016), Agència de Gestió d'Ajuts Universitaris i de Recerca (AGAUR) Grups de Recerca Emergents (SGR2009-1488; AGAUR-Catalan Government), and European Research Council (268692).eLife202420242014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/60190http://dx.doi.org/10.7554/eLife.02784reponame:Repositorio Digital de la UPFinstname:Universitat Pompeu FabraIngléseLife. 2014 Jun 17;3:e02784info:eu-repo/grantAgreement/EC/FP7/268692info:eu-repo/grantAgreement/ES/3PN/BFU2008-00414info:eu-repo/grantAgreement/ES/3PN/SGR2009-1488info:eu-repo/grantAgreement/ES/3PN/CSD2009-00016© 2014, Nogueira et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.http://creativecommons.org/licenses/by/3.0/info:eu-repo/semantics/openAccessoai:repositori.upf.edu:10230/601902026-06-12T07:21:37Z
dc.title.none.fl_str_mv SLY1 and syntaxin 18 specify a distinct pathway for procollagen VII export from the endoplasmic reticulum
title SLY1 and syntaxin 18 specify a distinct pathway for procollagen VII export from the endoplasmic reticulum
spellingShingle SLY1 and syntaxin 18 specify a distinct pathway for procollagen VII export from the endoplasmic reticulum
Nogueira, Cristina
Reticle endoplasmàtic
Col·lagen
Proteïnes
title_short SLY1 and syntaxin 18 specify a distinct pathway for procollagen VII export from the endoplasmic reticulum
title_full SLY1 and syntaxin 18 specify a distinct pathway for procollagen VII export from the endoplasmic reticulum
title_fullStr SLY1 and syntaxin 18 specify a distinct pathway for procollagen VII export from the endoplasmic reticulum
title_full_unstemmed SLY1 and syntaxin 18 specify a distinct pathway for procollagen VII export from the endoplasmic reticulum
title_sort SLY1 and syntaxin 18 specify a distinct pathway for procollagen VII export from the endoplasmic reticulum
dc.creator.none.fl_str_mv Nogueira, Cristina
Erlmann, Patrik
Villeneuve, Julien
Santos, António J. M.
Martínez-Alonso, Emma
Martínez-Menárguez, José Ángel
Malhotra, Vivek
author Nogueira, Cristina
author_facet Nogueira, Cristina
Erlmann, Patrik
Villeneuve, Julien
Santos, António J. M.
Martínez-Alonso, Emma
Martínez-Menárguez, José Ángel
Malhotra, Vivek
author_role author
author2 Erlmann, Patrik
Villeneuve, Julien
Santos, António J. M.
Martínez-Alonso, Emma
Martínez-Menárguez, José Ángel
Malhotra, Vivek
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Reticle endoplasmàtic
Col·lagen
Proteïnes
topic Reticle endoplasmàtic
Col·lagen
Proteïnes
description TANGO1 binds and exports Procollagen VII from the endoplasmic reticulum (ER). In this study, we report a connection between the cytoplasmic domain of TANGO1 and SLY1, a protein that is required for membrane fusion. Knockdown of SLY1 by siRNA arrested Procollagen VII in the ER without affecting the recruitment of COPII components, general protein secretion, and retrograde transport of the KDEL-containing protein BIP, and ERGIC53. SLY1 is known to interact with the ER-specific SNARE proteins Syntaxin 17 and 18, however only Syntaxin 18 was required for Procollagen VII export. Neither SLY1 nor Syntaxin 18 was required for the export of the equally bulky Procollagen I from the ER. Altogether, these findings reveal the sorting of bulky collagen family members by TANGO1 at the ER and highlight the existence of different export pathways for secretory cargoes one of which is mediated by the specific SNARE complex containing SLY1 and Syntaxin 18.
publishDate 2014
dc.date.none.fl_str_mv 2014
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/60190
http://dx.doi.org/10.7554/eLife.02784
url http://hdl.handle.net/10230/60190
http://dx.doi.org/10.7554/eLife.02784
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv eLife. 2014 Jun 17;3:e02784
info:eu-repo/grantAgreement/EC/FP7/268692
info:eu-repo/grantAgreement/ES/3PN/BFU2008-00414
info:eu-repo/grantAgreement/ES/3PN/SGR2009-1488
info:eu-repo/grantAgreement/ES/3PN/CSD2009-00016
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/3.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv eLife
publisher.none.fl_str_mv eLife
dc.source.none.fl_str_mv reponame:Repositorio Digital de la UPF
instname:Universitat Pompeu Fabra
instname_str Universitat Pompeu Fabra
reponame_str Repositorio Digital de la UPF
collection Repositorio Digital de la UPF
repository.name.fl_str_mv
repository.mail.fl_str_mv
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