Application of low-intensity pulsed therapeutic ultrasound on mesenchymal precursors does not affect their cell properties.

Ultrasound is considered a safe and non-invasive tool in regenerative medicine and has been used in the clinic for more than twenty years for applications in bone healing after the approval of the Exogen device, also known as low-intensity pulsed ultrasound (LIPUS). Beyond its effects on bone health...

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Detalles Bibliográficos
Autores: de Lucas, Beatriz, Perez, Laura M., Bernal, Aurora, Galvez, Beatriz G.
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/13348
Acceso en línea:http://hdl.handle.net/20.500.12105/13348
Access Level:acceso abierto
Palabra clave:Ultrasonic Therapy
Ultrasonic Waves
Animals
Blotting, Western
Cell Movement
Cytoskeleton
Humans
Mesenchymal Stem Cells
Mice
Microscopy, Fluorescence
Real-Time Polymerase Chain Reaction
Transcriptome
Wound Healing
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spelling Application of low-intensity pulsed therapeutic ultrasound on mesenchymal precursors does not affect their cell properties.de Lucas, BeatrizPerez, Laura M.Bernal, AuroraGalvez, Beatriz G.Ultrasonic TherapyUltrasonic WavesAnimalsBlotting, WesternCell MovementCytoskeletonHumansMesenchymal Stem CellsMiceMicroscopy, FluorescenceReal-Time Polymerase Chain ReactionTranscriptomeWound HealingUltrasound is considered a safe and non-invasive tool in regenerative medicine and has been used in the clinic for more than twenty years for applications in bone healing after the approval of the Exogen device, also known as low-intensity pulsed ultrasound (LIPUS). Beyond its effects on bone health, LIPUS has also been investigated for wound healing of soft tissues, with positive results for various cell processes including cell proliferation, migration and angiogenesis. As LIPUS has the potential to treat chronic skin wounds, we sought to evaluate the effects produced by a conventional therapeutic ultrasound device at low intensities (also considered LIPUS) on the migration capacity of mouse and human skin mesenchymal precursors (s-MPs). Cells were stimulated for 3 days (20 minutes per day) using a traditional ultrasound device with the following parameters: 100 mW/cm2 with 20% duty cycle and frequency of 3 MHz. At the parameters used, ultrasound failed to affect s-MP proliferation, with no evident changes in morphology or cell groupings, and no changes at the cytoskeletal level. Further, the migration and invasion ability of s-MPs were unaffected by the ultrasound protocol, and no major changes were detected in the gene/protein expression of ROCK1, integrin β1, laminin β1, type I collagen and transforming growth factor β1. Finally, RNA-seq analysis revealed that only 10 genes were differentially expressed after ultrasound stimulation. Among them, 5 encode for small nuclear RNAs and 2 encode for proteins belonging to the nuclear pore complex. Considering the results overall, while the viability of s-MPs was not affected by ultrasound stimulation and no changes were detected in proliferation/migration, RNA-seq analysis would suggest that s-MPs do respond to ultrasound. The use of 100 mW/cm2 intensity or conventional therapeutic ultrasound devices might not be optimal for the stimulation the properties of cell populations. Future studies should investigate the potential application of ultrasound using variations of the tested parameters.Public Library of Science (PLOS)20212021-09-0220212021-02-0120212021-02-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfapplication/octet-streamhttp://hdl.handle.net/20.500.12105/13348reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/133482026-06-12T12:43:37Z
dc.title.none.fl_str_mv Application of low-intensity pulsed therapeutic ultrasound on mesenchymal precursors does not affect their cell properties.
title Application of low-intensity pulsed therapeutic ultrasound on mesenchymal precursors does not affect their cell properties.
spellingShingle Application of low-intensity pulsed therapeutic ultrasound on mesenchymal precursors does not affect their cell properties.
de Lucas, Beatriz
Ultrasonic Therapy
Ultrasonic Waves
Animals
Blotting, Western
Cell Movement
Cytoskeleton
Humans
Mesenchymal Stem Cells
Mice
Microscopy, Fluorescence
Real-Time Polymerase Chain Reaction
Transcriptome
Wound Healing
title_short Application of low-intensity pulsed therapeutic ultrasound on mesenchymal precursors does not affect their cell properties.
title_full Application of low-intensity pulsed therapeutic ultrasound on mesenchymal precursors does not affect their cell properties.
title_fullStr Application of low-intensity pulsed therapeutic ultrasound on mesenchymal precursors does not affect their cell properties.
title_full_unstemmed Application of low-intensity pulsed therapeutic ultrasound on mesenchymal precursors does not affect their cell properties.
title_sort Application of low-intensity pulsed therapeutic ultrasound on mesenchymal precursors does not affect their cell properties.
dc.creator.none.fl_str_mv de Lucas, Beatriz
Perez, Laura M.
Bernal, Aurora
Galvez, Beatriz G.
author de Lucas, Beatriz
author_facet de Lucas, Beatriz
Perez, Laura M.
Bernal, Aurora
Galvez, Beatriz G.
author_role author
author2 Perez, Laura M.
Bernal, Aurora
Galvez, Beatriz G.
author2_role author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Ultrasonic Therapy
Ultrasonic Waves
Animals
Blotting, Western
Cell Movement
Cytoskeleton
Humans
Mesenchymal Stem Cells
Mice
Microscopy, Fluorescence
Real-Time Polymerase Chain Reaction
Transcriptome
Wound Healing
topic Ultrasonic Therapy
Ultrasonic Waves
Animals
Blotting, Western
Cell Movement
Cytoskeleton
Humans
Mesenchymal Stem Cells
Mice
Microscopy, Fluorescence
Real-Time Polymerase Chain Reaction
Transcriptome
Wound Healing
description Ultrasound is considered a safe and non-invasive tool in regenerative medicine and has been used in the clinic for more than twenty years for applications in bone healing after the approval of the Exogen device, also known as low-intensity pulsed ultrasound (LIPUS). Beyond its effects on bone health, LIPUS has also been investigated for wound healing of soft tissues, with positive results for various cell processes including cell proliferation, migration and angiogenesis. As LIPUS has the potential to treat chronic skin wounds, we sought to evaluate the effects produced by a conventional therapeutic ultrasound device at low intensities (also considered LIPUS) on the migration capacity of mouse and human skin mesenchymal precursors (s-MPs). Cells were stimulated for 3 days (20 minutes per day) using a traditional ultrasound device with the following parameters: 100 mW/cm2 with 20% duty cycle and frequency of 3 MHz. At the parameters used, ultrasound failed to affect s-MP proliferation, with no evident changes in morphology or cell groupings, and no changes at the cytoskeletal level. Further, the migration and invasion ability of s-MPs were unaffected by the ultrasound protocol, and no major changes were detected in the gene/protein expression of ROCK1, integrin β1, laminin β1, type I collagen and transforming growth factor β1. Finally, RNA-seq analysis revealed that only 10 genes were differentially expressed after ultrasound stimulation. Among them, 5 encode for small nuclear RNAs and 2 encode for proteins belonging to the nuclear pore complex. Considering the results overall, while the viability of s-MPs was not affected by ultrasound stimulation and no changes were detected in proliferation/migration, RNA-seq analysis would suggest that s-MPs do respond to ultrasound. The use of 100 mW/cm2 intensity or conventional therapeutic ultrasound devices might not be optimal for the stimulation the properties of cell populations. Future studies should investigate the potential application of ultrasound using variations of the tested parameters.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-09-02
2021
2021-02-01
2021
2021-02-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/13348
url http://hdl.handle.net/20.500.12105/13348
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/octet-stream
dc.publisher.none.fl_str_mv Public Library of Science (PLOS)
publisher.none.fl_str_mv Public Library of Science (PLOS)
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869419928527831040
score 15,198674

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