Fluorescently labelled pepino mosaic virus strains share infected tissues and colocalize in cellular aggregates reminiscent of viral replication complexes

Mixed infections of pepino mosaic virus (PepMV) isolates from the EU and CH2 strains are frequent in tomato crops. An asymmetric antagonistic relationship has been described between these strains, making their in planta interaction worthy of study. The aim of this work was to verify if PepMV isolate...

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Detalles Bibliográficos
Autores: Alcaide Cabello, Cristina, Aranda, Miguel A.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/352692
Acceso en línea:http://hdl.handle.net/10261/352692
Access Level:acceso abierto
Palabra clave:PepMV
Protoplasts
Virus antagonism
Virus accumulation
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spelling Fluorescently labelled pepino mosaic virus strains share infected tissues and colocalize in cellular aggregates reminiscent of viral replication complexesAlcaide Cabello, CristinaAranda, Miguel A.PepMVProtoplastsVirus antagonismVirus accumulationMixed infections of pepino mosaic virus (PepMV) isolates from the EU and CH2 strains are frequent in tomato crops. An asymmetric antagonistic relationship has been described between these strains, making their in planta interaction worthy of study. The aim of this work was to verify if PepMV isolates labelled with fluorescent proteins recapitulate the interactions described for wild type isolates and, if so, to determine the proportion of cells infected with each isolate in single and mixed infected plants. Infectious clones were prepared for PepMV-CH2-GFP and -EU-TagRFP, and also for their reciprocal combination, PepMV-CH2-TagRFP and -EU-GFP, and used to inoculate Nicotiana benthamiana plants. The accumulation of viral RNA followed trends that differed from wild type viruses, with the PepMV-EU-GFP and -CH2-TagRFP pair reproducing more closely the wild type interaction. Protoplasts were isolated from leaves that were systemically infected with PepMV-EU-GFP, -CH2-TagRFP, or both, and flow cytometry was used to determine the proportion of cells infected with each tagged isolate. A significant proportion (16.6%) of cells were found to be infected with both, without strong evidence of virus exclusion in coinfections, as could have been expected for related viruses; in fact, cellular structures reminiscent of viral replication complexes were found to be labelled with both fluorescent reportersWe acknowledge financial support from the Spanish Ministry of Science and Innovation through grants RTI2018–097099-B-I00 and PID2021–125010OB-I00Peer reviewedElsevier BVMinisterio de Ciencia e Innovación (España)Agencia Estatal de Investigación (España)Aranda, Miguel A. [0000-0002-0828-973X]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/352692reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-097099-B-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-125010OB-I00The underlying dataset has been published as supplementary material of the article in the publisher platform at https://doi.org/10.1016/j.virusres.2023.199100https://doi.org/10.1016/j.virusres.2023.199100Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3526922026-05-22T06:33:51Z
dc.title.none.fl_str_mv Fluorescently labelled pepino mosaic virus strains share infected tissues and colocalize in cellular aggregates reminiscent of viral replication complexes
title Fluorescently labelled pepino mosaic virus strains share infected tissues and colocalize in cellular aggregates reminiscent of viral replication complexes
spellingShingle Fluorescently labelled pepino mosaic virus strains share infected tissues and colocalize in cellular aggregates reminiscent of viral replication complexes
Alcaide Cabello, Cristina
PepMV
Protoplasts
Virus antagonism
Virus accumulation
title_short Fluorescently labelled pepino mosaic virus strains share infected tissues and colocalize in cellular aggregates reminiscent of viral replication complexes
title_full Fluorescently labelled pepino mosaic virus strains share infected tissues and colocalize in cellular aggregates reminiscent of viral replication complexes
title_fullStr Fluorescently labelled pepino mosaic virus strains share infected tissues and colocalize in cellular aggregates reminiscent of viral replication complexes
title_full_unstemmed Fluorescently labelled pepino mosaic virus strains share infected tissues and colocalize in cellular aggregates reminiscent of viral replication complexes
title_sort Fluorescently labelled pepino mosaic virus strains share infected tissues and colocalize in cellular aggregates reminiscent of viral replication complexes
dc.creator.none.fl_str_mv Alcaide Cabello, Cristina
Aranda, Miguel A.
author Alcaide Cabello, Cristina
author_facet Alcaide Cabello, Cristina
Aranda, Miguel A.
author_role author
author2 Aranda, Miguel A.
author2_role author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Agencia Estatal de Investigación (España)
Aranda, Miguel A. [0000-0002-0828-973X]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv PepMV
Protoplasts
Virus antagonism
Virus accumulation
topic PepMV
Protoplasts
Virus antagonism
Virus accumulation
description Mixed infections of pepino mosaic virus (PepMV) isolates from the EU and CH2 strains are frequent in tomato crops. An asymmetric antagonistic relationship has been described between these strains, making their in planta interaction worthy of study. The aim of this work was to verify if PepMV isolates labelled with fluorescent proteins recapitulate the interactions described for wild type isolates and, if so, to determine the proportion of cells infected with each isolate in single and mixed infected plants. Infectious clones were prepared for PepMV-CH2-GFP and -EU-TagRFP, and also for their reciprocal combination, PepMV-CH2-TagRFP and -EU-GFP, and used to inoculate Nicotiana benthamiana plants. The accumulation of viral RNA followed trends that differed from wild type viruses, with the PepMV-EU-GFP and -CH2-TagRFP pair reproducing more closely the wild type interaction. Protoplasts were isolated from leaves that were systemically infected with PepMV-EU-GFP, -CH2-TagRFP, or both, and flow cytometry was used to determine the proportion of cells infected with each tagged isolate. A significant proportion (16.6%) of cells were found to be infected with both, without strong evidence of virus exclusion in coinfections, as could have been expected for related viruses; in fact, cellular structures reminiscent of viral replication complexes were found to be labelled with both fluorescent reporters
publishDate 2023
dc.date.none.fl_str_mv 2023
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/352692
url http://hdl.handle.net/10261/352692
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-097099-B-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-125010OB-I00
The underlying dataset has been published as supplementary material of the article in the publisher platform at https://doi.org/10.1016/j.virusres.2023.199100
https://doi.org/10.1016/j.virusres.2023.199100
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier BV
publisher.none.fl_str_mv Elsevier BV
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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