Fibroblasts enhance the growth and survival of adult feline small intestinal organoids

Intestinal Intestinal organoids are important cell culture models that complement live animal studies of many intestinal pathogens. Adult feline small intestinal organoids are needed for infectious disease research but are difficult to work with due to slow growth and premature senescence. We introd...

Full description

Bibliographic Details
Authors: Hryckowian, Nicole D., Studener, Katelyn, Frizzarini, Waneska S., Arranz Solís, David, Sánchez Sánchez, Roberto, Knoll, Laura J.
Format: article
Publication Date:2025
Country:España
Institution:Universidad Complutense de Madrid (UCM)
Repository:Docta Complutense
Language:English
OAI Identifier:oai:docta.ucm.es:20.500.14352/124005
Online Access:https://hdl.handle.net/20.500.14352/124005
Access Level:Open access
Keyword:636.09
Toxoplasma gondii
Enteroid
Feline
Fibroblast
Organoid
Small intestine
Veterinaria
3109 Ciencias Veterinarias
id ES_cddba3c3dc6d011ddbbb5aa49edc1238
oai_identifier_str oai:docta.ucm.es:20.500.14352/124005
network_acronym_str ES
network_name_str España
repository_id_str
spelling Fibroblasts enhance the growth and survival of adult feline small intestinal organoidsHryckowian, Nicole D.Studener, KatelynFrizzarini, Waneska S.Arranz Solís, DavidSánchez Sánchez, RobertoKnoll, Laura J.636.09Toxoplasma gondiiEnteroidFelineFibroblastOrganoidSmall intestineVeterinaria3109 Ciencias VeterinariasIntestinal Intestinal organoids are important cell culture models that complement live animal studies of many intestinal pathogens. Adult feline small intestinal organoids are needed for infectious disease research but are difficult to work with due to slow growth and premature senescence. We introduce a method of co-culturing adult feline small intestinal organoids with growth-inhibited human foreskin fibroblast feeder cells to enhance organoid proliferation and survival. With feeder cells, feline jejunal and ileal organoids survived at least 9 months in culture until cryopreservation. Fibroblast supplementation increased the maximum size of cat and mouse intestinal organoids as well. The increased longevity and size of these organoids are a significant improvement on current methods. These organoids also supported pre-sexual development of the medically important parasite Toxoplasma gondii, as evidenced by expression of the merozoite-specific marker GRA11b. This GRA11b positivity was higher in mature cat organoid-derived monolayers grown for 21 days prior to infection, compared with monolayers grown for 10 days. These methods have high potential to reduce the number of cats used for infectious disease research and may be applicable for intestinal cells from other animals that are difficult to culture.IMPORTANCEMany microbial pathogens are acquired orally through contaminated food or water. Being able to model these infections in cell culture has been greatly enhanced by the development of intestinal organoid technology. One of the species that hosts several infections is cats, but cat intestinal organoids have been notoriously difficult to grow. Here, we describe a co-culture method with fibroblast cells that dramatically improves the longevity of adult cat intestinal organoids. These cat organoid cells can support the pre-sexual development stages of the intestinal pathogen Toxoplasma gondii, a parasite whose sexual cycle is restricted to cats and is the reason that pregnant women are told not to change the litter box. These culture conditions will be a resource to study other cat intestinal pathogens and intestinal organoids from other animals that are difficult to cultureAmerican Society for MicrobiologyUniversidad Complutense de Madrid20252025-01-0120252025-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/124005reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1240052026-06-02T12:44:21Z
dc.title.none.fl_str_mv Fibroblasts enhance the growth and survival of adult feline small intestinal organoids
title Fibroblasts enhance the growth and survival of adult feline small intestinal organoids
spellingShingle Fibroblasts enhance the growth and survival of adult feline small intestinal organoids
Hryckowian, Nicole D.
636.09
Toxoplasma gondii
Enteroid
Feline
Fibroblast
Organoid
Small intestine
Veterinaria
3109 Ciencias Veterinarias
title_short Fibroblasts enhance the growth and survival of adult feline small intestinal organoids
title_full Fibroblasts enhance the growth and survival of adult feline small intestinal organoids
title_fullStr Fibroblasts enhance the growth and survival of adult feline small intestinal organoids
title_full_unstemmed Fibroblasts enhance the growth and survival of adult feline small intestinal organoids
title_sort Fibroblasts enhance the growth and survival of adult feline small intestinal organoids
dc.creator.none.fl_str_mv Hryckowian, Nicole D.
Studener, Katelyn
Frizzarini, Waneska S.
Arranz Solís, David
Sánchez Sánchez, Roberto
Knoll, Laura J.
author Hryckowian, Nicole D.
author_facet Hryckowian, Nicole D.
Studener, Katelyn
Frizzarini, Waneska S.
Arranz Solís, David
Sánchez Sánchez, Roberto
Knoll, Laura J.
author_role author
author2 Studener, Katelyn
Frizzarini, Waneska S.
Arranz Solís, David
Sánchez Sánchez, Roberto
Knoll, Laura J.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 636.09
Toxoplasma gondii
Enteroid
Feline
Fibroblast
Organoid
Small intestine
Veterinaria
3109 Ciencias Veterinarias
topic 636.09
Toxoplasma gondii
Enteroid
Feline
Fibroblast
Organoid
Small intestine
Veterinaria
3109 Ciencias Veterinarias
description Intestinal Intestinal organoids are important cell culture models that complement live animal studies of many intestinal pathogens. Adult feline small intestinal organoids are needed for infectious disease research but are difficult to work with due to slow growth and premature senescence. We introduce a method of co-culturing adult feline small intestinal organoids with growth-inhibited human foreskin fibroblast feeder cells to enhance organoid proliferation and survival. With feeder cells, feline jejunal and ileal organoids survived at least 9 months in culture until cryopreservation. Fibroblast supplementation increased the maximum size of cat and mouse intestinal organoids as well. The increased longevity and size of these organoids are a significant improvement on current methods. These organoids also supported pre-sexual development of the medically important parasite Toxoplasma gondii, as evidenced by expression of the merozoite-specific marker GRA11b. This GRA11b positivity was higher in mature cat organoid-derived monolayers grown for 21 days prior to infection, compared with monolayers grown for 10 days. These methods have high potential to reduce the number of cats used for infectious disease research and may be applicable for intestinal cells from other animals that are difficult to culture.IMPORTANCEMany microbial pathogens are acquired orally through contaminated food or water. Being able to model these infections in cell culture has been greatly enhanced by the development of intestinal organoid technology. One of the species that hosts several infections is cats, but cat intestinal organoids have been notoriously difficult to grow. Here, we describe a co-culture method with fibroblast cells that dramatically improves the longevity of adult cat intestinal organoids. These cat organoid cells can support the pre-sexual development stages of the intestinal pathogen Toxoplasma gondii, a parasite whose sexual cycle is restricted to cats and is the reason that pregnant women are told not to change the litter box. These culture conditions will be a resource to study other cat intestinal pathogens and intestinal organoids from other animals that are difficult to culture
publishDate 2025
dc.date.none.fl_str_mv 2025
2025-01-01
2025
2025-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/124005
url https://hdl.handle.net/20.500.14352/124005
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869419893182431232
score 15,811543

Similar Items