The Effect of 5 Proteins On DPPSC (Dental Pulp Pluripotent Stem Cells) For Osteoblast Differentiation Proliferation In 3D
Bone-tissue engineering is a therapeutic target in the field of dental implant and orthopaedic surgery. It is therefore essential to find a microenvironment that enhances the growth and differentiation of osteoblasts both from mesenchymal stem cells (MSCs) and those derived from dental pulp (DPSC)....
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2014 |
| País: | España |
| Institución: | CBUC, CESCA |
| Repositorio: | TDR. Tesis Doctorales en Red |
| OAI Identifier: | oai:www.tdx.cat:10803/277382 |
| Acceso en línea: | http://hdl.handle.net/10803/277382 |
| Access Level: | acceso abierto |
| Palabra clave: | Dental pulp pluripotent stem cell Dental pulp mesenchymal stem cell Fibronectin Bone morphogenic protein 2 Osteopontin Tenascin Bone sialoprotein Odontologia 616.3 |
| Sumario: | Bone-tissue engineering is a therapeutic target in the field of dental implant and orthopaedic surgery. It is therefore essential to find a microenvironment that enhances the growth and differentiation of osteoblasts both from mesenchymal stem cells (MSCs) and those derived from dental pulp (DPSC). The aim of this research is to determine the relationship among the proteins fibronectin (FN), bone morphogenetic protein 2 (BMP2) osteopontin (OPN), tenascin C (TNC) and bone sialoprotein (BSP) and their ability to coat different types of biomaterials and surfaces to enhance osteoblast differentiation. To evaluate the osteogenic capacities of dental pulp pluripotent stem cells (DPPSC) and dental pulp mesenchymal stem cells (DPMSC), DPPSC and DPMSC were cultured on bio-coating of 5 different types of proteins 20 days. We performed quantification of alkaline phosphatase and Ca2+ in the medium and RT-PCR with osteogenic marker (Col I, BMP2 and OCN). SEM images corresponding to OPN and TNC group were performed. After that we tested the strength of scaffold in all groups. The result represents DPPSC seeded on 3d scaffold coated with OPN has the highest osteoinduction. DPMSC seeded on 3d scaffold coated with BMP2 has highest osteoinduction. We suggest that DPMSC is more suitable for bone regeneration than DPPSC. However, for dental implant placement or trauma, we prefer trabecular bone with great blood supply, thus, bone regeneration from DPPSC may get a better result. DPPSC is a new strategy to regenerate bone. We are coming closer to provide bone regeneration by using DPPSC. |
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